Mechanism of TiO2 nanotube UV-photocatalytic degradation of antibiotic resistance genes in the wastewater sludge and blocking of the transfer

Frontiers in Environmental Science, Journal Year: 2025, Volume and Issue: 13

Published: May 6, 2025

Antibiotic resistance genes (ARGs) in sludge propagate via horizontal gene transfer (HGT), necessitating advanced mitigation strategies. This study demonstrates TiO 2 nanotube UV photocatalysis effectively degrades ARGs (70.6–82.5% reduction) and suppresses HGT by targeting mobile genetic elements (MGEs; 93.4–97.1% removal). Hierarchical nanotubes (anatase phase) generated reactive oxygen species (ROS) inducing oxidative DNA damage cell lysis, preferentially eliminating intracellular (33.5–46.6% decline) while converting them to extracellular forms. Mobile (MGEs) ( tnpA-04 / intI1 ) were selectively fragmented ROS, outperforming HOCl-based systems. Microbial analysis revealed Proteobacteria (e.g., Kofleria as key ARG hosts, whose decline correlated with reduction p <0.05). Radiation-resistant Deinococcus dominated post-treatment communities but lacked associations, indicating non-transmissible residual risks. Spatial-specific degradation mechanisms emerged: directly chromosomal ARGs, ROS oxidized plasmid-borne MGEs, achieving dual elimination blockade. The intracellular-to-extracellular shift host-MGE decoupling confirmed transmission disruption. work establishes a paradigm for treatment, synchronizing removal environmental risk through ROS-microbe-DNA interplay.

Language: Английский

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Optimizing fermentation pH in food waste systems: a dual strategy to amplify microbial diversity and carbon source productivity

Journal of environmental chemical engineering, Journal Year: 2025, Volume and Issue: unknown, P. 117111 - 117111

Published: May 1, 2025

Language: Английский

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Enhanced volatile fatty acids production in thermal hydrolysis pretreatment with constant pH regulation: Effects evaluation and mechanism elucidation

Journal of environmental chemical engineering, Journal Year: 2024, Volume and Issue: 12(6), P. 114830 - 114830

Published: Nov. 17, 2024

Language: Английский

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