Cascade DNA Structural Transitions Enable Stimuli-Responsive Hydrogels DOI
Maria Ho, Ashley Lim, Yujie Ke

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: April 25, 2025

Cascade interactions are fundamental to enzyme catalysis and cellular activities, enabling dynamic adaptive responses environmental stimuli. DNA-based cascade systems have been widely employed mimic biological processes, such as immune DNAzyme catalysis, achieved mainly through the hybridization interaction. Herein, we present a DNA system involving single-stranded sequences, noncanonical cofactor-bridged duplexes, canonical duplexes construct dissociate hydrogel matrices. In this work, thymine-rich oligonucleotides (T-strands) exist random coils in buffer at pH 7.2. Upon introduction of low-molecular-weight cofactor, melamine (MA), supramolecular configuration, termed T-MA-T duplex, is formed. Subsequent addition adenine-rich (A-strands) leads replacement MA cofactors formation more energetically favorable A-T duplex structures. These consecutive structural transitions further utilized bridging elements stimuli-responsive hydrogels, facilitating liquid-hydrogel-liquid phase transitions. Moreover, demonstrate precisely controlled release profiles doxorubicin from hydrogel. This approach, leveraging both configurations triggered transitions, opens avenues for developing molecular switches, electronic nanodevices, materials, other advanced applications.

Language: Английский

APP-PER-MEL Aerogel based on Supramolecular Assembly: New Insight from Conventional Concept DOI
Dan Wang, Chang Wang, Tingting Li

et al.

Polymer Degradation and Stability, Journal Year: 2025, Volume and Issue: 234, P. 111197 - 111197

Published: Jan. 16, 2025

Language: Английский

Citations

0
A DNA-Based Plasmonic Nano-Ruler DOI Open Access

Aura Cencini,

Mary Bortoluzzi,

Graziano Rilievo

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2557 - 2557

Published: March 12, 2025

DNA is an exceptional building block for the fabrication of dynamic supramolecular systems with switchable geometries. Here, a self-assembled, tunable plasmonic–fluorescent nanostructure was developed. A precise sliding motion mechanism operated through control strand displacement reactions, shifting two single-strand (ssDNA) rails connected by ssDNA quasi-ring structure. The system reconfigured as nano-mechanical structure, generating six discrete configurations, and setting specific distances between tethered gold nanoparticle (AuNP) fluorophore, Sulfo-Cyanine3 (Cy3). Each configuration produced distinct fluorescence emission intensity via plasmonic quenching/enhancement effects, therefore structure behaved nano-ruler. To optimize system, reversible distance-dependent quenching or enhancement phenomena were investigated testing AuNPs diameters 5, 10, 15 nm, yielding best performances 10 nm AuNPs. Furthermore, geometric model produced, confirming observed results. fluorophore–plasmonic surface positioning, conferred ruler, led to finite state nano-machine alternative signal outputs. This mechanism, working fluorescent reporter, could find application in multiple-responsive detection nucleic acids, such viruses microRNAs.

Language: Английский

Citations

0
Cascade DNA Structural Transitions Enable Stimuli-Responsive Hydrogels DOI
Maria Ho, Ashley Lim, Yujie Ke

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: April 25, 2025

Cascade interactions are fundamental to enzyme catalysis and cellular activities, enabling dynamic adaptive responses environmental stimuli. DNA-based cascade systems have been widely employed mimic biological processes, such as immune DNAzyme catalysis, achieved mainly through the hybridization interaction. Herein, we present a DNA system involving single-stranded sequences, noncanonical cofactor-bridged duplexes, canonical duplexes construct dissociate hydrogel matrices. In this work, thymine-rich oligonucleotides (T-strands) exist random coils in buffer at pH 7.2. Upon introduction of low-molecular-weight cofactor, melamine (MA), supramolecular configuration, termed T-MA-T duplex, is formed. Subsequent addition adenine-rich (A-strands) leads replacement MA cofactors formation more energetically favorable A-T duplex structures. These consecutive structural transitions further utilized bridging elements stimuli-responsive hydrogels, facilitating liquid-hydrogel-liquid phase transitions. Moreover, demonstrate precisely controlled release profiles doxorubicin from hydrogel. This approach, leveraging both configurations triggered transitions, opens avenues for developing molecular switches, electronic nanodevices, materials, other advanced applications.

Language: Английский

Citations

0