Single BMSC-derived cartilage organoids for gradient heterogeneous osteochondral regeneration by leveraging native vascular microenvironment DOI Creative Commons
Zhenying Chen,

Qitao Bo,

Chao Wang

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: April 29, 2025

Heterogeneous osteochondral regeneration remains a significant challenge due to the distinct microenvironments across cartilage, calcified and subchondral bone layers. The natural gradient of vascularization from superficial deep layers tissue plays critical role in guiding differentiation marrow stem cells (BMSCs) into chondrocytes osteoblasts. In this study, we propose strategy for heterogeneous using cartilage organoids derived single BMSCs, leveraging within tissue. We successfully isolated BMSCs rabbits generated via vitro three-dimensional chondrogenic culture. To mimic pro-vascular microenvironment, introduced vascular endothelial growth factor, which promoted hypertrophic organoids. then prepared organoid/GelMA complexes, with or without anti-vascular drug Axitinib, implanted them subcutaneously nude mice. vascularized subcutaneous microenvironment induced osteogenic differentiation, while Axitinib treatment created an inhibiting osteogenesis preserving chondrogenesis complexes. Both vivo data demonstrated crucial regulating Finally, cylinders were rabbit defect, where at defect site guided differentiate both bone. This BMSC-derived organoid approach enables precise regeneration, by site, representing advancement clinical applications.

Language: Английский

Gradient scaffolds in bone-soft tissue interface engineering: Structural characteristics, fabrication techniques, and emerging trends DOI Creative Commons
Ying Liu, Wan Yue, Chaojing Li

et al.

Journal of Orthopaedic Translation, Journal Year: 2025, Volume and Issue: 50, P. 333 - 353

Published: Jan. 1, 2025

Bone and soft tissues are connected by a complex interface that is crucial for the smooth transfer of mechanical stress. Effective repair this requires bio-scaffolds specifically designed to support regeneration diverse cell types signalling molecules. With advances in micro- nanotechnologies, gradient biomaterial scaffolds have demonstrated significant potential tissue regeneration. This paper reviews structure bone-soft interface, various scaffold types, construction methods. It also discusses recent developments future directions engineering, emphasizing restore natural function interfaces. Overall, provides valuable insights into application offering inspiration biomimetic approaches soft-hard medical engineering. First, emphasis on could significantly impact clinical practices related integration, ultimately improving patient outcomes quality life. Second, it aligns with growing trend potentially inspiring new innovations strategies.

Language: Английский

Citations

1
Bio-inspired mineralized collagen scaffolds with precisely controlled gradients for the treatment of severe osteoarthritis in a male rabbit model DOI
Lili Wang, Xian Chen, Xiaoyan Wang

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 139843 - 139843

Published: Jan. 1, 2025

Language: Английский

Citations

0
Continuous magnetic-gradient hydrogel with augmented mechanical span and reverse-directional polysaccharides distribution for integrated repair of osteochondral defects DOI
Junwei Xu, Yi Cui, Xuemei Sun

et al.

Composites Part B Engineering, Journal Year: 2025, Volume and Issue: unknown, P. 112361 - 112361

Published: March 1, 2025

Language: Английский

Citations

0
Bioinspired Paste‐Extrusion Printed Microlattices with Natural Bone‐Like Porosity and Performance DOI Open Access

Xianwen Wang,

Haolei Wang, Yetao Li

et al.

Small, Journal Year: 2025, Volume and Issue: unknown

Published: March 10, 2025

The structure feature determines its performance. In the field of biological implants, microlattices are commonly used as building blocks for light-weight and adaptive purposes, which however show limitations in mechanical properties compared with natural bones. Inspired by efficient mass transfer high fault tolerance neural networks derived from hierarchical functional gradient, a bioinspired paste-extrusion printed microlattice (BPPM) is developed tunable demonstrated. non-crossing structures first verified outweigh crossing one under equivalent compressive stress. Then, introducing gradient components 3D printing process, BPPM porosity, composites fabricated. As result, shows eliminated deformation along direction, fine surface roughness (Sa 3.65-15.67 µm), wide range porosity (56-78%) strength (3.44-22.3 MPa), favorable permeability (3.02 × 103-3.22 103D), good biocompatibility promoted cell proliferation. This work not only demonstrates bones but also provides robust way to realize it.

Language: Английский

Citations

0
Bi-phasic integrated silk fibroin/polycaprolactone scaffolds for osteochondral regeneration inspired by the native joint tissue and interface DOI Creative Commons

Z Zhang,

Qingquan Dong,

Zubing Li

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: unknown, P. 101737 - 101737

Published: April 1, 2025

Osteochondral scaffolds designed with bi-phasic and multi-phasic have typically struggled post-implantation delamination. To address this issue, we developed a novel integrated scaffold natural continuous interface heterogeneous bilayer structure. Through layer-by-layer wet electrospinning, two-dimensional (2D) bi-layer membranes of silk fibroin (SF) polycaprolactone (PCL) were fabricated. These then transformed into three-dimensional (3D) using CO2 gas foaming technique, followed by gelatin coating on the osteogenic layer to afford final porous scaffolds. In vitro studies indicated that 3D better-maintained cell phenotypes than conventional 2D electrospun films. Additionally, showed superior cartilage repair osteoinductivity potential, increased subchondral bone volume reduced defect area in rat osteochondral defects models at 12 weeks. Taken together, these gas-foamed promising candidate for regeneration.

Language: Английский

Citations

0
Single BMSC-derived cartilage organoids for gradient heterogeneous osteochondral regeneration by leveraging native vascular microenvironment DOI Creative Commons
Zhenying Chen,

Qitao Bo,

Chao Wang

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: April 29, 2025

Heterogeneous osteochondral regeneration remains a significant challenge due to the distinct microenvironments across cartilage, calcified and subchondral bone layers. The natural gradient of vascularization from superficial deep layers tissue plays critical role in guiding differentiation marrow stem cells (BMSCs) into chondrocytes osteoblasts. In this study, we propose strategy for heterogeneous using cartilage organoids derived single BMSCs, leveraging within tissue. We successfully isolated BMSCs rabbits generated via vitro three-dimensional chondrogenic culture. To mimic pro-vascular microenvironment, introduced vascular endothelial growth factor, which promoted hypertrophic organoids. then prepared organoid/GelMA complexes, with or without anti-vascular drug Axitinib, implanted them subcutaneously nude mice. vascularized subcutaneous microenvironment induced osteogenic differentiation, while Axitinib treatment created an inhibiting osteogenesis preserving chondrogenesis complexes. Both vivo data demonstrated crucial regulating Finally, cylinders were rabbit defect, where at defect site guided differentiate both bone. This BMSC-derived organoid approach enables precise regeneration, by site, representing advancement clinical applications.

Language: Английский

Citations

0