Thermoresponsive Injectable Microsphere Glycopeptide Hydrogels for Remodeling Dynamic Cell Microenvironments

ACS Applied Polymer Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 24, 2025

Language: Английский

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Real-time quantification of microfluidic hydrogel crosslinking via gas-phase electrophoresis

Journal of Colloid and Interface Science, Journal Year: 2025, Volume and Issue: 684, P. 201 - 212

Published: Jan. 7, 2025

Language: Английский

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Enhancing Immunomodulation and Osseointegration of Bone Implants via Thrombin‐Activated Platelet‐Rich Plasma Self‐Assembly

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

Abstract Platelet‐rich plasma (PRP) is characterized by elevated concentrations of growth factors that facilitate bone repair. Nonetheless, the effective integration PRP with implants and sustained release its active constituents pose significant challenges. In this study, thrombin grafted onto surface polyetheretherketone (PEEK) via an N,N'‐Disuccinimidyl Carbonate (DSC) linker retained enzymatic activity enables controlled activation self‐assembly, resulting in formation a functional bio‐gel layer. The optimal concentration to be 100 U/ mL −1 determined, at which point both grafting amount reaches their peak, no further increases observed higher concentrations. solutions varying platelet enrichment ratios are subsequently activated on thrombin‐grafted PEEK surface, yielding self‐assembled bio‐gels capable factor for up 16 days. thrombin‐activated not only enhances vitro cell adhesion, proliferation, osteogenic differentiation, vascularization specific polarization macrophages, but also effectively facilitates vivo angiogenesis, immunomodulation dose‐dependent manner. Consequently, gel presents promising strategy biological functionalization orthopedic applications.

Language: Английский

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Spheroid‐on‐a‐Chip Platforms for Tumor Microenvironment and Drug Development

Advanced Materials Technologies, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 12, 2025

Abstract Despite significant advancements in oncology research and therapeutic interventions, cancer continues to be the leading cause of mortality worldwide. The key challenge addressing this pressing issue lies lack precision diagnosis a limited understanding nature how tumor microenvironment responds interventions. Research focusing on impact (TME) heterogeneity response drugs is crucial ensure efficient therapy. Conventional models exhibit constraints, including inability accurately imitate tumors’ complex 3D architecture dynamic microenvironment. Recent developments Spheroid‐On‐a‐Chip (SoC) technology provide alternative, offering closer that human tissue. This review explores recent SoC modeling, highlighting spheroid formation mechanisms techniques. applications platform mimic essential features their potential role anticancer drug development are summarized. advantages challenges technologies behind devices compared traditional vitro further discussed. Lastly, future directions for transforming improving suggested.

Language: Английский

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From Microsphere Synthesis to Neural Tissue Regeneration: Unraveling the Potentials and Progress

Composites Part B Engineering, Journal Year: 2025, Volume and Issue: unknown, P. 112363 - 112363

Published: March 1, 2025

Language: Английский

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Sequential construction of vascularized and mineralized bone organoids using engineered ECM-DNA-CPO-based bionic matrix for efficient bone regeneration

Bioactive Materials, Journal Year: 2025, Volume and Issue: 49, P. 362 - 377

Published: March 14, 2025

Language: Английский

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Hyaluronic Acid-Based Hydrogel Microspheres with Multi-Responsive Properties for Antibacterial Therapy and Bone Regeneration in Staphylococcus aureus-Infected Skull Defects

Materials Today Bio, Journal Year: 2025, Volume and Issue: 32, P. 101676 - 101676

Published: March 27, 2025

This study introduces hyaluronic acid-based (HA) hydrogel microspheres loaded with zinc oxide nanoparticles (ZnO-NPs) for the treatment of infectious bone defects. The were fabricated using a 3D-printing process, formulation consisting 6 wt% HAD (methacrylated HA), 3 AOHA (AMP-conjugated oxidized 1 % BOHA (phenylboric acid-conjugated 0.5 photoinitiator, and 0.05 ZnO-NPs. In vitro, demonstrated significant antibacterial activity against Staphylococcus aureus, colony counts biofilm inhibition assays showing marked reduction in bacterial growth after 12 24 h. release antimicrobial peptides (AMPs) was enhanced acidic conditions presence hyaluronidase. also promoted osteogenic differentiation marrow stromal cells (BMSCs), as evidenced by increased expression markers (ALP, OCN, OPN, COL-1). vivo, tested rat skull defect model, regeneration, improved angiogenesis, an anti-inflammatory response. These results indicate that ABOHA@ZnO provide promising strategy treating defects combining antimicrobial, osteogenic.

Language: Английский

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Rapid Fabrication of Diverse Hydrogel Microspheres for Drug Evaluation on a Rotating Microfluidic System

Langmuir, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

Hydrogel microspheres are considered ideal carriers with broad applications in 3D cell culture, drug delivery, and microtissue construction. Although multiple methods have been developed for generating hydrogel microspheres, there is still a lack of universal approach that combines operability, stability, cost-effectiveness, biocompatibility. In this work, novel rotating microfluidic system (RMS) proposed, which can rapidly fabricate diverse poly(ethylene glycol) diacrylate/sodium alginate (PEGDA/SA) by motor-driven rotation the oil phase to form special T-shaped structure needle. The main part consists commercially available motors, beaker, needles do not require precision machining user-friendly low cost. Moreover, adjusting parameters such as needle structure, flow rate, rotational speed, platform enables rapid fabrication different sizes cores, including crescent, thick wavy, oval, spherical. Furthermore, tumor cell-laden hyaluronic acid methacrylate/sodium (HAMA/SA) were fabricated using system, demonstrated good viability proliferation subsequent culture. vitro evaluation models cisplatin revealed potential evaluation. These results indicated RMS has other culture-based biomedical applications.

Language: Английский

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ROS‐Activated Nanohydrogel Scaffolds with Multi‐Factors Controlled Release for Targeted Dual‐Lineage Repair of Osteochondral Defects

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 29, 2025

Abstract Achieving self‐healing for osteochondral defects caused by trauma, aging, or disease remains a significant challenge in clinical practice. It is an effective therapeutic strategy to construct gradient‐biomimetic biomaterials that replicate the hierarchical structure and complex microenvironment of tissues dual‐lineage regeneration both cartilage subchondral bone. Herein, ROS‐activated nanohydrogels composite bilayer scaffolds with multi‐factors controlled release are rationally designed using combination 3D printing gelatin placeholder methods. The resulting nanohydrogel exhibit micro‐nano interconnected porous soft‐hard mechanical strength facilitating culture BMSCs vitro. More importantly, multi‐stage continuous responses anti‐inflammation, chondrogenesis osteogenesis, effectively induced via sequential multi‐factors, including diclofenac sodium (DS), kartogenin (KGN) bone morphogenetic protein 2 (BMP‐2), from scaffolds, thereby improved tissue defect model SD rats. These findings suggest such specific delivery functional factors, provides promising defects.

Language: Английский

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MXene‐Based Photothermal‐Responsive Injectable Hydrogel Microsphere Modulates Physicochemical Microenvironment to Alleviate Osteoarthritis

Smart Medicine, Journal Year: 2025, Volume and Issue: 4(2)

Published: April 13, 2025

ABSTRACT Osteoarthritis (OA) is a physical lubrication microenvironment‐inadequate disease accompanied by sustained chronic chemical inflammation microenvironment and the progression of articular cartilage destruction. Despite promising OA treatment outcomes observed in enhancement inspired ball bearings to reduce friction support loads, therapeutic effect near‐infrared (NIR) irradiation‐based photothermal‐responsive controlled release “smart hydrogel microspheres” on remains unclear. Here, we prepared MXene/NIPIAM‐based injectable microspheres encapsulating diclofenac sodium using microfluidic system. Consequently, NIR demonstrate beneficial effects modulating microenvironment, laying foundation for application smart microsphere delivery systems loaded with bioactive factors (including agents, cells, factors) regulate multiple pathological microenvironments regenerative medicine.

Language: Английский

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