Exploring Nucleic Acid Nanozymes: A New Frontier in Biosensor Development

Biosensors, Journal Year: 2025, Volume and Issue: 15(3), P. 142 - 142

Published: Feb. 24, 2025

With the growing interest in nucleic acids and nanozymes, acid nanozymes (NANs) have emerged as a promising alternative to traditional enzyme catalysts, combining advantages of nanomaterials, are widely applied field biosensing. This review provides comprehensive overview recent studies on NAN-based biosensors. It classifies NANs based six distinct enzymatic activities: peroxidase-like, oxidase-like, catalase-like, superoxide dismutase-like, laccase-like, glucose oxidase-like. emphasizes how catalytic activity is significantly influenced by properties explores regulatory mechanisms governing NANs. Additionally, it systematically reviews important research progress colorimetric, fluorescent, electrochemical, SERS, chemiluminescent sensors, offering insights into development NAN biosensor applications.

Language: Английский

Advancing Tumor Microenvironment Analysis: A Fluorescence Nanosystem for Caspase-1 Monitoring and Synergistic Therapy

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

The lack of precise, real-time analytical tools for monitoring tumor microenvironment changes during treatment hinders advancements in integrated diagnostic and therapeutic platforms. Traditional caspase-3 strategies are limited by their inability to address drug resistance newly discovered apoptotic pathways, leading reduced accuracy practicality. To overcome these limitations, we developed a fluorescence-based "Trojan horse" nanosystem, PFpR@CM, featuring high-sensitivity Caspase-1 detection, tumor-targeted delivery, photothermal therapy. was selected as biomarker due its ability provide accurate feedback on reactive oxygen species (ROS) generation. system employs Fe-doped polydopamine nanoparticles red fluorescent carbon quantum dots (RCQDs) the core, achieving detection limit 0.024 U/mL with linear range 0.05-1.0 U/mL. By integrating MG-63 cell membrane camouflage, PFpR@CM ensures specificity immune evasion, allowing precise situ ROS production ferroptosis. Experimental results demonstrate that enables simultaneous fluorescence tracking localized interventions, over 80% volume reduction vivo minimal systemic toxicity. This work establishes novel chemistry approach multifunctional treatment, providing an innovative solution challenges precision oncology.

Language: Английский

A novel electrochemical strategy for rapid and sensitive α-Glucosidase activity detection and inhibitor discovery based on distinct oxidation potentials of substrate and product

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113678 - 113678

Published: April 1, 2025

Language: Английский