Remodeling the Neuroimmune Microenvironment in Retinal Ischemia‐Reperfusion Injury via Combined CRISPR/Cas9 Targeting of ACSL4 and Isorhamnetin DOI
Wen Zhang, Lin Li,

Lusheng Ma

et al.

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 27, 2025

Abstract Retinal ischemia‐reperfusion (RIR) injury induces oxidative stress, excitotoxicity, inflammation, and ferroptosis, which interact through complex crosstalk, forming a retinal pathological microenvironment (RPMe) that drives ganglion cell (RGC) death. Central to these processes is the dysregulation of neuroimmune (NiMe), characterized by aberrant microglial activation around RGCs immune signaling imbalances. Here, analysis single‐cell RNA sequencing, it identifies significant sphingolipid pathway in RIR‐injured microglia, crosstalks with pathways. This crosstalk disrupts NiMe homeostasis. To address this, biomimetic nanoparticle system coated precursor membranes developed. co‐delivers CRISPR/Cas9‐based Acyl‐CoA synthetase long‐chain family member 4 (ACSL4) inhibitor suppress ferroptosis isorhamnetin, natural molecule identified directly bind protein kinase A inhibit glutamatergic synapse involved stress. By targeting its PI3K/AKT ASK1/JNK/NF‐κB pathways as well RGCs, this restores balance. The membrane‐coated offers novel, synergistic, targeted therapeutic strategy for RIR‐related diseases integrating CRISPR/Cas9 technology product therapy.

Language: Английский

Evaluation of Anti-Inflammatory and Antibacterial Properties of Photo-Thermal Hydrogel as Dual Functional Platform for Management of Periodontitis DOI Creative Commons

Zhihong Mai,

Y.S. Mai,

Xianxian Huang

et al.

International Journal of Nanomedicine, Journal Year: 2025, Volume and Issue: Volume 20, P. 2923 - 2934

Published: March 1, 2025

Background: Periodontitis, one of the most common oral diseases caused by bacterial infection which affects gums, periodontal ligament and alveolar bone, is a leading cause tooth loss in adults. Current clinical treatments, such as scaling or antibiotics, often result incomplete biofilm removal can contribute to drug resistance. Methods: To address these limitations, nanozyme comprising platinum, copper, selenium was developed, then incorporated into thermoresponsive hydrogel. Results: When applied pockets exposed 808 nm laser irradiation, hydrogel became soft, dissolving release nanozyme. This demonstrated superoxide dismutase (SOD)-like catalase (CAT)-like activity, reducing excess reactive oxygen species (ROS) while displaying strong antibacterial anti-inflammatory effects. Conclusion: photothermal showed excellent biocompatibility has potential overcome challenges current periodontitis treatments. Keywords: thermosensitive, therapy, hydrogel,

Language: Английский

Citations

1
Ni–Cu Bimetallic Nanozyme and Minoxidil Co-Loaded Dissolving Microneedles Reshape Hair Follicle Microenvironment for Androgenic Alopecia Treatment DOI
Beibei Wang,

Mengcheng Tang,

Yongjia Zhang

et al.

ACS Applied Nano Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 28, 2025

Language: Английский

Citations

0
Remodeling the Neuroimmune Microenvironment in Retinal Ischemia‐Reperfusion Injury via Combined CRISPR/Cas9 Targeting of ACSL4 and Isorhamnetin DOI
Wen Zhang, Lin Li,

Lusheng Ma

et al.

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 27, 2025

Abstract Retinal ischemia‐reperfusion (RIR) injury induces oxidative stress, excitotoxicity, inflammation, and ferroptosis, which interact through complex crosstalk, forming a retinal pathological microenvironment (RPMe) that drives ganglion cell (RGC) death. Central to these processes is the dysregulation of neuroimmune (NiMe), characterized by aberrant microglial activation around RGCs immune signaling imbalances. Here, analysis single‐cell RNA sequencing, it identifies significant sphingolipid pathway in RIR‐injured microglia, crosstalks with pathways. This crosstalk disrupts NiMe homeostasis. To address this, biomimetic nanoparticle system coated precursor membranes developed. co‐delivers CRISPR/Cas9‐based Acyl‐CoA synthetase long‐chain family member 4 (ACSL4) inhibitor suppress ferroptosis isorhamnetin, natural molecule identified directly bind protein kinase A inhibit glutamatergic synapse involved stress. By targeting its PI3K/AKT ASK1/JNK/NF‐κB pathways as well RGCs, this restores balance. The membrane‐coated offers novel, synergistic, targeted therapeutic strategy for RIR‐related diseases integrating CRISPR/Cas9 technology product therapy.

Language: Английский

Citations

0