Bioorthogonal Engineering of Bacterial Outer Membrane Vesicles for NIR-II Fluorescence Imaging-Guided Synergistic Enhanced Immunotherapy DOI
Na Li, Minghui Wang, Fen Liu

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(49), P. 19585 - 19596

Published: Nov. 27, 2024

The efficacy of immunotherapy in treating triple-negative breast cancer (TNBC) has been restricted due to its low immunogenicity and suppressive immune microenvironment. Bacterial outer membrane vesicles (OMVs) have emerged as innovative immunotherapeutic agents antitumor therapy by stimulating the innate system, but intricate modifications undesirable multiple dose administration severely hinder their utility. Herein, a two-step bacterial metabolic labeling technique was utilized for bioorthogonal engineering OMVs. At first, d-propargylglycine (DPG, an alkyne-containing d-amino acid) introduced into incubation process probiotic Escherichia coli 1917 (Ecn) produce DPG-functionalized OMVs, which were subsequently conjugated with azide-functionalized new indocyanine green (IR820) yield OMV-DPG-IR820. combination phototherapy immunostimulation OMV-DPG-IR820 effectively arouses adaptive responses, causing maturation dendritic cells, infiltration T repolarization M2 macrophage M1 macrophage, upregulation inflammatory factors. Remarkably, demonstrated tumor-targeting capabilities guidance provided near-infrared II (NIR-II) fluorescence imaging, leading remarkable inhibition on both primary distant tumors preventing metastasis without noticeable adverse reactions. This study elucidates sophisticated strategy design production functionalized OMVs provides novel perspectives microbiome-mediated reversal TNBC through precise efficient immunotherapy.

Language: Английский

Microneedle patch-involved local therapy synergized with immune checkpoint inhibitor for pre- and post-operative cancer treatment DOI
Yue Yin, Lu Tang,

Yuqi Cao

et al.

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: 379, P. 678 - 695

Published: Jan. 24, 2025

Language: Английский

Citations

1
A Nano-Strategy for Advanced Triple-Negative Breast Cancer Therapy by Regulating Intratumoral Microbiota DOI
Jifan Gao, Lu Tang,

Cong Fu

et al.

Nano Letters, Journal Year: 2025, Volume and Issue: unknown

Published: April 3, 2025

Intratumoral microbiota have been identified as a component of the tumor microenvironment that regulates metastatic behavior tumors. They serve not only indicators pathology but also potential drug targets in cancer therapy. Herein, multifunctional nanoplatform (DD@FEL) is prepared by combining antibiotic doxycycline (DOXY) can combat intratumoral and chemotherapeutic doxorubicin (DOX) ergosterol-originated liposome. Specially, ergosterol utilized substitute for cholesterol liposomes to exert pharmacological activity. Mechanistically, DD@FEL leveraged DOXY inhibit metastasis based on regulation microbiota, which synergizes with effect DOX, eventually inhibiting progression triple-negative breast (TNBC). Verified both vitro vivo, effectively exerts cytotoxic TNBC cells, delays growth primary TNBC, attenuates development its lung metastasis, providing promising therapeutic strategy control orthotopic TNBC.

Language: Английский

Citations

0
Treating and Protecting against Recurrent Vulvovaginal Candidiasis Using the Vaginal Epithelial Cell Membrane-Based Photoimmunotherapeutic Nanoplatform DOI
Ledan Wang,

Yijing Lin,

Shuangshuang Liu

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

Recurrent vulvovaginal candidiasis (RVVC) is an opportunistic infection predominantly caused by Candida albicans (C. albicans) and particularly prevalent among individuals on immunosuppressants. Currently, there are no FDA-approved therapies for specifically controlling RVVC, mainly due to the need therapeutics against RVVC that require both antifungal treatments resolve active infections strategies prevent recurrence. This study introduces a biomimetic photoimmunotherapeutic nanoplatform consisting of adjuvant-encapsulated polymeric core stabilized photosensitizer-loaded vaginal epithelial cell membrane coating treat protect RVVC. With its camouflaging, nanoplatforms target enhance adherence intravaginal site C. infection, allowing resist being flushed away fluids. Upon subsequent near-infrared irradiation, nanoplatform's targeted photothermal power effectively eliminates while minimizing thermal damage surrounding healthy tissue. Postphotothermal treatment, generated albicans-based debris candidalysin-captured (serving as nanotoxoid), along with adjuvant, processed resident antigen-presenting cells promote multiantigenic immunity. response provides protection secondary (RVVC model) albicans-induced systemic even under immunosuppressive conditions (septicemia model). Notably, anti-C. antibodies produced in pretreated mice exhibit comparable affinity clinically isolated strains, indicating potential clinical application. Overall, this underscores proposed effective treatment prevention

Language: Английский

Citations

0
Bioorthogonal Engineering of Bacterial Outer Membrane Vesicles for NIR-II Fluorescence Imaging-Guided Synergistic Enhanced Immunotherapy DOI
Na Li, Minghui Wang, Fen Liu

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(49), P. 19585 - 19596

Published: Nov. 27, 2024

The efficacy of immunotherapy in treating triple-negative breast cancer (TNBC) has been restricted due to its low immunogenicity and suppressive immune microenvironment. Bacterial outer membrane vesicles (OMVs) have emerged as innovative immunotherapeutic agents antitumor therapy by stimulating the innate system, but intricate modifications undesirable multiple dose administration severely hinder their utility. Herein, a two-step bacterial metabolic labeling technique was utilized for bioorthogonal engineering OMVs. At first, d-propargylglycine (DPG, an alkyne-containing d-amino acid) introduced into incubation process probiotic Escherichia coli 1917 (Ecn) produce DPG-functionalized OMVs, which were subsequently conjugated with azide-functionalized new indocyanine green (IR820) yield OMV-DPG-IR820. combination phototherapy immunostimulation OMV-DPG-IR820 effectively arouses adaptive responses, causing maturation dendritic cells, infiltration T repolarization M2 macrophage M1 macrophage, upregulation inflammatory factors. Remarkably, demonstrated tumor-targeting capabilities guidance provided near-infrared II (NIR-II) fluorescence imaging, leading remarkable inhibition on both primary distant tumors preventing metastasis without noticeable adverse reactions. This study elucidates sophisticated strategy design production functionalized OMVs provides novel perspectives microbiome-mediated reversal TNBC through precise efficient immunotherapy.

Language: Английский

Citations

1