Vascular smooth muscle cells transdifferentiate into chondrocyte-like cells and facilitate meniscal fibrocartilage regeneration DOI Creative Commons

Wenqiang Yan,

Cheng Jin, Haoda Wu

et al.

Research, Journal Year: 2024, Volume and Issue: 7

Published: Jan. 1, 2024

The effective and translational strategy to regenerate knee meniscal fibrocartilage remained challenging. Herein, we first identified vascular smooth muscle cells (VSMCs) transdifferentiated into fibrochondrocytes participated in spontaneous regeneration using cell lineage tracing transgenic mice defect model. Then, low-intensity pulsed ultrasound (LIPUS) acoustic stimulus enhanced fibrochondrogenic transdifferentiation of VSMCs vitro vivo. Mechanistically, LIPUS could up-regulate mechanosensitive ion channel Piezo1 expression then activate the transforming growth factor β1 (TGFβ1) signal, following repression Notch consequently enhancing VSMCs. Finally, demonstrated that regular anisotropic native-like tissue a beagle canine subtotal meniscectomy model at 6 months postoperatively. single-cell RNA sequencing analysis confirmed role VSMC regeneration.

Language: Английский

A novel composite meniscus scaffold with shape memory and adhesive properties alleviates knee cartilage degeneration through minimally invasive and sutureless approach DOI Creative Commons

Wenqiang Yan,

Feng Tian, Lingyu Zhao

et al.

Advanced Composites and Hybrid Materials, Journal Year: 2025, Volume and Issue: 8(3)

Published: May 14, 2025

Language: Английский

Citations

0
Vascular smooth muscle cells transdifferentiate into chondrocyte-like cells and facilitate meniscal fibrocartilage regeneration DOI Creative Commons

Wenqiang Yan,

Cheng Jin, Haoda Wu

et al.

Research, Journal Year: 2024, Volume and Issue: 7

Published: Jan. 1, 2024

The effective and translational strategy to regenerate knee meniscal fibrocartilage remained challenging. Herein, we first identified vascular smooth muscle cells (VSMCs) transdifferentiated into fibrochondrocytes participated in spontaneous regeneration using cell lineage tracing transgenic mice defect model. Then, low-intensity pulsed ultrasound (LIPUS) acoustic stimulus enhanced fibrochondrogenic transdifferentiation of VSMCs vitro vivo. Mechanistically, LIPUS could up-regulate mechanosensitive ion channel Piezo1 expression then activate the transforming growth factor β1 (TGFβ1) signal, following repression Notch consequently enhancing VSMCs. Finally, demonstrated that regular anisotropic native-like tissue a beagle canine subtotal meniscectomy model at 6 months postoperatively. single-cell RNA sequencing analysis confirmed role VSMC regeneration.

Language: Английский

Citations

0