High-Performance Biosensing Platforms Based on Enzyme-Linked Nucleic Acid Amplification Regulated by Synergistic Allosteric Hairpin Catalysis of Bimetallic Nanozymes and Its Mechanisms DOI
Yue Ma, Changwen Jin,

Feiyan Yan

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

Sugar cane smut disease can greatly decrease both the production and quality of sugar cane, its early diagnosis is an effective strategy to ensure increase income cane. Therefore, developing high-precision detection methods has major implications for actual Herein, we synthesize bimetallic nanozymes Fe3O4@AuNPs with excellent glucose oxidase-like activity nitrogen-doped graphdiyne (N-GDY) conductivity interfacial loading capacity, which are used as catalysts biofuel cells flexible electrode substrates, respectively. An allosteric hairpin-regulated enzymatic cascade nucleic acid amplification employed construct a novel biosensing platform precise highly sensitive analysis pathogen causing disease, catalytic mechanism studied. The specifically cause complementary region hairpin migrate form new functional hairpin. Under promotion enzymes, dual occurs using template outputs large amount double-stranded products, captured by RCA long chain on biocathode. At cathode, DNA double strands capable holding quantity Ru[(NH3)6]3+ through electrostatic attraction. anode catalyze oxidation produce electrons, AuNPs/N-GDY efficiently transfer electrons cathode obtain strong open-circuit voltage signal, exhibits linear correlation in range 0.0001-10000 pM, limit 53.29 aM (S/N = 3). sensing offers reliable method that allows accurate great application development potential identification on-site rapid detection.

Language: Английский

Advances of functional graphdiyne in separation and detection DOI
Weibin Wang, Youyong Li, Xiaohua Xiao

et al.

Talanta, Journal Year: 2025, Volume and Issue: 287, P. 127673 - 127673

Published: Jan. 30, 2025

Language: Английский

Citations

0
Nano-enabled biosensors in early detection of plant diseases DOI Creative Commons

Ambika Chaturvedi,

Deepti Tripathi, Rajiv Ranjan

et al.

Frontiers in Nanotechnology, Journal Year: 2025, Volume and Issue: 7

Published: April 4, 2025

Plant disease outbreaks are raising concerns about global food security. Pathogenic evolution and continuous climate changes increase the threat to agriculture necessitate surveillance. To prevent future maintain agricultural sustainability advanced tools required. Nowadays various types of nanobiosensors such as electrochemical, piezoelectric, thermal, optical, Fluorescence resonance energy transfer (FRET)-based biosensors used predict disease-associated pathogens, toxins, abiotic stress. Nanobiosensors, provide quick detection diseases may protect from pandemics they overcome time dependency traditional methods real-time monitoring. The incorporation nanoparticles with chitosan nanoparticles, silver (AgNPs), gold (AuNPs), multiwalled carbon nanotubes (MWCNTs), graphene oxide, etc., facilitates precise pesticides, disease-causing pathogens in plants. Furthermore, integration portable devices artificial intelligence (AI) increases their practical application Despite promising aspect, issues sensor stability, large-scale development, cost-effectiveness also need be addressed. Future studies more concerned improving durability, multiplex ability, user-friendly field application. enhance output, it is necessary develop an early diagnosis approach that heavily dependent on ongoing development cost-effective nanobiosensors. This review focuses recent operation mechanism for pathogen detection. Additionally, challenges associated worldwide acceptance nano Overall, new-edge technology enhances plant management strategies risk mitigation

Language: Английский

Citations

0
High-Performance Biosensing Platforms Based on Enzyme-Linked Nucleic Acid Amplification Regulated by Synergistic Allosteric Hairpin Catalysis of Bimetallic Nanozymes and Its Mechanisms DOI
Yue Ma, Changwen Jin,

Feiyan Yan

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

Sugar cane smut disease can greatly decrease both the production and quality of sugar cane, its early diagnosis is an effective strategy to ensure increase income cane. Therefore, developing high-precision detection methods has major implications for actual Herein, we synthesize bimetallic nanozymes Fe3O4@AuNPs with excellent glucose oxidase-like activity nitrogen-doped graphdiyne (N-GDY) conductivity interfacial loading capacity, which are used as catalysts biofuel cells flexible electrode substrates, respectively. An allosteric hairpin-regulated enzymatic cascade nucleic acid amplification employed construct a novel biosensing platform precise highly sensitive analysis pathogen causing disease, catalytic mechanism studied. The specifically cause complementary region hairpin migrate form new functional hairpin. Under promotion enzymes, dual occurs using template outputs large amount double-stranded products, captured by RCA long chain on biocathode. At cathode, DNA double strands capable holding quantity Ru[(NH3)6]3+ through electrostatic attraction. anode catalyze oxidation produce electrons, AuNPs/N-GDY efficiently transfer electrons cathode obtain strong open-circuit voltage signal, exhibits linear correlation in range 0.0001-10000 pM, limit 53.29 aM (S/N = 3). sensing offers reliable method that allows accurate great application development potential identification on-site rapid detection.

Language: Английский

Citations

0