
Desalination and Water Treatment, Journal Year: 2025, Volume and Issue: unknown, P. 101176 - 101176
Published: April 1, 2025
Language: Английский
Desalination and Water Treatment, Journal Year: 2025, Volume and Issue: unknown, P. 101176 - 101176
Published: April 1, 2025
Language: Английский
Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 489, P. 137696 - 137696
Published: Feb. 20, 2025
Language: Английский
Citations
0Water Research, Journal Year: 2025, Volume and Issue: unknown, P. 123407 - 123407
Published: Feb. 1, 2025
Language: Английский
Citations
0Journal of Environmental Management, Journal Year: 2025, Volume and Issue: 383, P. 125327 - 125327
Published: April 21, 2025
The anaerobic bacterium Megasphaera hexanoica has great potential for caproate production through reverse β-oxidation using lactate, acetate, and butyrate as substrates. This study assessed the effect of acetate concentrations (10-50 mM) on biosynthesis, lactate oxidation, cell synthesis in M. hexanoica. At 30 mM reached 42.83 mM, with an electron efficiency 67.03 % a specific productivity 4.47 gCA·h-1·gDCW-1. Subsequent fed-batch experiments maintained continuous production, achieving 65.21 mM. Bioreactor assays further validated strategy, yielding 65.25 over 180 h. Mechanistic analysis demonstrated that optimized acetyl-CoA flux enhanced caproyl-CoA transferase activity (3.5 U‧mg-1), supporting synthesis. Kinetic modeling Logistic model fit consumption (R2 = 0.991), while Fitzhugh captured 0.991, NRMSE 1.137). findings offer practical insights industrial-scale production.
Language: Английский
Citations
0Desalination and Water Treatment, Journal Year: 2025, Volume and Issue: unknown, P. 101176 - 101176
Published: April 1, 2025
Language: Английский
Citations
0