Resveratrol-Loaded Solid Lipid Nanoparticles Reinforced Hyaluronic Hydrogel: Multitarget Strategy for the Treatment of Diabetes-Related Periodontitis DOI Creative Commons
Raffaele Conte, Anna Valentino,

Fabrizia Sepe

et al.

Biomedicines, Journal Year: 2025, Volume and Issue: 13(5), P. 1059 - 1059

Published: April 27, 2025

Background/Objectives: Periodontitis and diabetes mellitus share a well-established bidirectional relationship, where hyperglycemia exacerbates periodontal inflammation, disease further impairs glycemic control. Within the diabetic microenvironment, an imbalance between pro-inflammatory (M1) anti-inflammatory (M2) macrophages promotes chronic oxidative stress, delayed healing, alveolar bone resorption. Resveratrol (RSV), polyphenol with antioxidant, anti-inflammatory, pro-osteogenic properties, holds potential to restore macrophage balance. However, its clinical application is limited by poor bioavailability instability. This study aimed develop evaluate novel RSV delivery system overcome these limitations promote tissue regeneration under conditions. Methods: A drug comprising RSV-loaded solid lipid nanoparticles embedded within cross-linked hyaluronic acid hydrogel (RSV@CLgel) was formulated. The tested hyperglycemic inflammatory conditions for effects on polarization, cytokine expression, mitochondrial function, osteoblast differentiation. Results: RSV@CLgel effectively suppressed cytokines (TNF-α, IL-1β, IL-6) while upregulating markers (IL-10, TGF-β). It significantly reduced stress decreasing ROS peroxidation levels improved function antioxidant enzyme activity. Furthermore, enhanced differentiation, as evidenced increased ALP activity, calcium nodule formation, upregulation of osteogenic genes (COL-I, RUNX2, OCN, OPN). also inhibited RANKL-induced osteoclastogenesis, contributing preservation. Conclusions: presents promising multifunctional strategy management periodontitis. By modulating immune responses, reducing promoting regeneration, addresses key pathological aspects diabetes-associated disease.

Language: Английский

A Multirisk-Rescued Biomimetic Nanozyme Against Periodontitis Via Inflammation Targeting and Microenvironment Reprogramming DOI
Lin Li, Rui‐Xin Wu, Jia Wang

et al.

Published: Jan. 1, 2025

Periodontitis is an intractable chronic inflammatory disease characterized by excessive proinflammatory factors production and reactive oxygen species (ROS) accumulation. Nanodrugs have made significant contributions in the biomedical field but fall short as periodontitis therapeutics because of their singular effects, low retention rates, poor specificity, insufficient biocompatibility. Herein, we report a functionalized periodontal ligament stem cell (PDLSC) membrane-camouflaged MnO2 nanoplatform (MnO2@hPM) to target reprogram microenvironment periodontitis. PDLSC-affinity biological molecules hypoxia-educated proteins on membrane coating endow MnO2@hPM with capacities actively PDLSCs under environment, concurrently neutralize various factors, scavenge overburdened ROS. The synergistic effects inflammation inhibition ROS elimination mitigate mitochondrial dysfunction, improve metabolic disturbance, restore osteogenic potential inflammation-impaired PDLSCs. In vivo, effectively accumulates at sites, significantly ameliorates inflammation, alleviates bone loss artificial periodontitis, showing optimized therapeutic performance for tissue reconstruction. This multirisk-rescuing biomimetic nanozyme good biocompatibility exhibits inflammation-targeting ability benefits against highlighting its advanced therapy other diseases.

Language: Английский

Citations

0
A multirisk-rescued biomimetic nanozyme against periodontitis via inflammation targeting and microenvironment reprogramming DOI
Lin Li, Rui‐Xin Wu, Jia Wang

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: 506, P. 160119 - 160119

Published: Jan. 1, 2025

Language: Английский

Citations

0
Resveratrol-Loaded Solid Lipid Nanoparticles Reinforced Hyaluronic Hydrogel: Multitarget Strategy for the Treatment of Diabetes-Related Periodontitis DOI Creative Commons
Raffaele Conte, Anna Valentino,

Fabrizia Sepe

et al.

Biomedicines, Journal Year: 2025, Volume and Issue: 13(5), P. 1059 - 1059

Published: April 27, 2025

Background/Objectives: Periodontitis and diabetes mellitus share a well-established bidirectional relationship, where hyperglycemia exacerbates periodontal inflammation, disease further impairs glycemic control. Within the diabetic microenvironment, an imbalance between pro-inflammatory (M1) anti-inflammatory (M2) macrophages promotes chronic oxidative stress, delayed healing, alveolar bone resorption. Resveratrol (RSV), polyphenol with antioxidant, anti-inflammatory, pro-osteogenic properties, holds potential to restore macrophage balance. However, its clinical application is limited by poor bioavailability instability. This study aimed develop evaluate novel RSV delivery system overcome these limitations promote tissue regeneration under conditions. Methods: A drug comprising RSV-loaded solid lipid nanoparticles embedded within cross-linked hyaluronic acid hydrogel (RSV@CLgel) was formulated. The tested hyperglycemic inflammatory conditions for effects on polarization, cytokine expression, mitochondrial function, osteoblast differentiation. Results: RSV@CLgel effectively suppressed cytokines (TNF-α, IL-1β, IL-6) while upregulating markers (IL-10, TGF-β). It significantly reduced stress decreasing ROS peroxidation levels improved function antioxidant enzyme activity. Furthermore, enhanced differentiation, as evidenced increased ALP activity, calcium nodule formation, upregulation of osteogenic genes (COL-I, RUNX2, OCN, OPN). also inhibited RANKL-induced osteoclastogenesis, contributing preservation. Conclusions: presents promising multifunctional strategy management periodontitis. By modulating immune responses, reducing promoting regeneration, addresses key pathological aspects diabetes-associated disease.

Language: Английский

Citations

0