Reversible Stiffening of Biopolymeric Hybrid Networks by Dynamically Switching Cross-Links In Situ DOI Creative Commons

Jana Tamara Reh,

Sebastian Voigt,

Leonard R. Gareis

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: May 13, 2025

Achieving reversible stiffening of biopolymer networks in a controlled manner remains challenging topic materials science, especially when trying to assess the following changes mechanical material properties real time. To address these challenges, we here utilize custom-made measurement setup that allows us manipulate cross-linking state alginate-based hydrogels situ while quantifying achieved alterations viscoelastic response networks. Interpolymer connections are created by combination light-induced, covalent cross-links, ionic and DNA-based where latter two can be successfully removed again employing either chelating agents (e.g., ethylenediaminetetraacetic acid citrate) or suitable displacement DNA strands. In part, this range different options mentioned is inter alia made possible incorporating glycoprotein mucin into alginate system, which also for starting (∼0.2-400 Pa), intermediate (∼25 Pa-1.6 kPa), final stiffnesses (∼4 Pa-1.2 kPa) mixed hydrogel matrix. At same time, presence mucins (1-4% (w/v)) mixture enhances cytocompatible improving its antibacterial characteristics. Such well-controllable alginate/mucin with dynamically switchable will likely find broad applications cell cultivation studies tissue engineering applications.

Language: Английский

Reversible Stiffening of Biopolymeric Hybrid Networks by Dynamically Switching Cross-Links In Situ DOI Creative Commons

Jana Tamara Reh,

Sebastian Voigt,

Leonard R. Gareis

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: May 13, 2025

Achieving reversible stiffening of biopolymer networks in a controlled manner remains challenging topic materials science, especially when trying to assess the following changes mechanical material properties real time. To address these challenges, we here utilize custom-made measurement setup that allows us manipulate cross-linking state alginate-based hydrogels situ while quantifying achieved alterations viscoelastic response networks. Interpolymer connections are created by combination light-induced, covalent cross-links, ionic and DNA-based where latter two can be successfully removed again employing either chelating agents (e.g., ethylenediaminetetraacetic acid citrate) or suitable displacement DNA strands. In part, this range different options mentioned is inter alia made possible incorporating glycoprotein mucin into alginate system, which also for starting (∼0.2-400 Pa), intermediate (∼25 Pa-1.6 kPa), final stiffnesses (∼4 Pa-1.2 kPa) mixed hydrogel matrix. At same time, presence mucins (1-4% (w/v)) mixture enhances cytocompatible improving its antibacterial characteristics. Such well-controllable alginate/mucin with dynamically switchable will likely find broad applications cell cultivation studies tissue engineering applications.

Language: Английский

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