A deadly dance: the choreography of host–pathogen interactions, as revealed by single-cell technologies DOI Creative Commons
Pratip K. Chattopadhyay, Mario Roederer, Diane L. Bolton

et al.

Nature Communications, Journal Year: 2018, Volume and Issue: 9(1)

Published: Oct. 31, 2018

Abstract Pathogens have numerous mechanisms by which they replicate within a host, who in turn responds developing innate and adaptive immune countermeasures to limit disease. The advent of high-content single-cell technologies has facilitated greater understanding the properties host cells harboring infection, host’s pathogen-specific responses, pathogens evolved escape control. Here we review these advances argue for inclusion higher resolution into approaches defining evasion pathogens.

Language: Английский

Developmental plasticity allows outside-in immune responses by resident memory T cells DOI
Raíssa Fonseca, Lalit K. Beura,

Clare F. Quarnstrom

et al.

Nature Immunology, Journal Year: 2020, Volume and Issue: 21(4), P. 412 - 421

Published: Feb. 17, 2020

Language: Английский

Citations

220
Tissue-resident memory CD8+ T cells amplify anti-tumor immunity by triggering antigen spreading through dendritic cells DOI Creative Commons
Evelyn Menares, Felipe Gálvez‐Cancino, Pablo Cáceres-Morgado

et al.

Nature Communications, Journal Year: 2019, Volume and Issue: 10(1)

Published: Sept. 27, 2019

Abstract Tissue-resident memory CD8 + T (Trm) cells mediate potent local innate and adaptive immune responses play a central role against solid tumors. However, whether Trm cross-talk with dendritic (DCs) to support anti-tumor immunity remains unclear. Here we show that antigen-specific activation of skin leads maturation migration draining lymph nodes cross-presenting dermal DCs. Tumor rejection mediated by triggers the spread cytotoxic cell tumor-derived neo- self-antigens via These suppress growth intradermal tumors disseminated melanoma lacking cell-targeted epitope. Moreover, analysis RNA sequencing data from human reveals enrichment gene signature associates DC improved survival. This work unveils ability amplify breath through DCs, thereby strengthening immunity.

Language: Английский

Citations

188
Parenteral BCG vaccine induces lung-resident memory macrophages and trained immunity via the gut–lung axis DOI Creative Commons

Mangalakumari Jeyanathan,

Maryam Vaseghi‐Shanjani,

Sam Afkhami

et al.

Nature Immunology, Journal Year: 2022, Volume and Issue: 23(12), P. 1687 - 1702

Published: Dec. 1, 2022

Aside from centrally induced trained immunity in the bone marrow (BM) and peripheral blood by parenteral vaccination or infection, evidence indicates that mucosal-resident innate immune memory can develop via a local inflammatory pathway following mucosal exposure. However, whether results integrating distally generated immunological signals vaccination/infection is unclear. Here we show subcutaneous Bacillus Calmette-Guérin (BCG) induce alveolar macrophages (AMs) lung. Although BCG trains BM progenitors circulating monocytes, induction of AMs independent monocytes. Rather, vaccination, mycobacterial dissemination, causes time-dependent alteration intestinal microbiome, barrier function microbial metabolites, subsequent changes lung leading to These data identify an microbiota-mediated for development at distal tissues have implications next-generation vaccine strategies against respiratory pathogens.

Language: Английский

Citations

84
Depleting myeloid-biased haematopoietic stem cells rejuvenates aged immunity DOI
Jason Ross, Lara Myers, Joseph J. Noh

et al.

Nature, Journal Year: 2024, Volume and Issue: 628(8006), P. 162 - 170

Published: March 27, 2024

Language: Английский

Citations

77
Integration of Single-Cell RNA-Seq Datasets: A Review of Computational Methods DOI Open Access
Daehee Hwang, Geun Hee Han, Eun‐Soo Jung

et al.

Molecules and Cells, Journal Year: 2023, Volume and Issue: 46(2), P. 106 - 119

Published: Feb. 1, 2023

With the increased number of single-cell RNA sequencing (scRNA-seq) datasets in public repositories, integrative analysis multiple scRNA-seq has become commonplace.Batch effects among different are inevitable because differences cell isolation and handling protocols, library preparation technology, platforms.To remove these batch for effective integration datasets, a methodologies have been developed based on diverse concepts approaches.These methods proven useful examining whether cellular features, such as subpopulations marker genes, identified from certain dataset, consistently present, or their conditiondependent variations, increases particular disease-related conditions, observed generated under similar distinct conditions.In this review, we summarize approaches pros cons reported previous literature.

Language: Английский

Citations

46
A pairwise cytokine code explains the organism-wide response to sepsis DOI Creative Commons
Michihiro Takahama, Ashwini Patil,

Gabriella Richey

et al.

Nature Immunology, Journal Year: 2024, Volume and Issue: 25(2), P. 226 - 239

Published: Jan. 8, 2024

Abstract Sepsis is a systemic response to infection with life-threatening consequences. Our understanding of the molecular and cellular impact sepsis across organs remains rudimentary. Here, we characterize pathogenesis by measuring dynamic changes in gene expression organs. To pinpoint molecules controlling organ states sepsis, compare effects on those 6 singles 15 pairs recombinant cytokines. Strikingly, find that pairwise tumor necrosis factor plus interleukin (IL)-18, interferon-gamma or IL-1β suffice mirror tissues. Mechanistically, map cytokines computing abundance 195 cell types 9 organs, which validate whole-mouse spatial profiling. work decodes cytokine cacophony into message capturing gene, tissue responses host disease.

Language: Английский

Citations

20
Armed and Ready: Transcriptional Regulation of Tissue-Resident Memory CD8 T Cells DOI Creative Commons
Felix M. Behr, Ammarina Beumer‐Chuwonpad, Regina Stark

et al.

Frontiers in Immunology, Journal Year: 2018, Volume and Issue: 9

Published: July 30, 2018

A fundamental benefit of immunological memory is the ability to respond in an enhanced manner upon secondary encounter with same pathogen. Tissue-resident CD8 T (TRM) cells contribute improved protection against reinfection through generation immediate effector responses at site pathogen entry. Key potential TRM develop rapid recall their location within epithelia skin, lungs, and intestines prime entry sites pathogens. are among first immune pathogens that have been previously encountered antigen-specific manner. Upon recognition invading pathogens, release IFN-γ other pro-inflammatory cytokines chemokines. These molecules activate surrounding epithelial tissue recruit including natural killer (NK) cells, B circulating infection. The repertoire functions also includes direct lysis infected cytotoxic such as perforin granzymes. mechanisms enabling a gradually being uncovered. In this review, we will address signals instruct maintenance well underlying transcriptional network keeps deployment-ready modus. Furthermore, discuss how transcription factors may control proliferative responses.

Language: Английский

Citations

110
Niches for the Long-Term Maintenance of Tissue-Resident Memory T Cells DOI Creative Commons
Shiki Takamura

Frontiers in Immunology, Journal Year: 2018, Volume and Issue: 9

Published: May 31, 2018

Tissue-resident memory T cells (TRM cells) are a population of immune that reside in the lymphoid and non-lymphoid organs without recirculation through blood. These important occupy utilize unique anatomical physiological niches distinct from those for other cell populations, such as central (TCM secondary effector (TEM circulate tissues. CD8+ TRM typically localize epithelial layers barrier tissues where they optimally positioned to act sentinels trigger antigen-specific protection against reinfection. CD4+ below layers, basement membrane, cluster structures designed optimize interactions with antigen presenting upon A key feature populations is their ability be maintained prolonged periods time. For example, skin displace epidermal originally occupied by cells, thereby enabling stable persistence years. It also clear long-term maintenance different microenvironments dependent on multiple tissue-specific survival cues, although specific details poorly understood. However, not all persist over long term. Recently we identified new spatial niche lung, which created at site tissue regeneration after injury (termed repair-associated depots; RAMD). The short-lived nature RAMD potentially explains short lifespans this particular tissue. Clearly, better understanding niche-dependent will development vaccines promote immunity. In review, discuss recent advances our properties maintain

Language: Английский

Citations

108
Innate immune memory of tissue-resident macrophages and trained innate immunity: Re-vamping vaccine concept and strategies DOI
Zhou Xing,

Sam Afkhami,

Jegarubee Bavananthasivam

et al.

Journal of Leukocyte Biology, Journal Year: 2020, Volume and Issue: 108(3), P. 825 - 834

Published: March 3, 2020

In the past few years, our understanding of immunological memory has evolved remarkably due to a growing body new knowledge in innate immune and immunity. Immunological now encompasses both adaptive memory. The hypo-reactive hyper-reactive types lead suppressed enhanced protective outcome, respectively. latter is also named trained immunity (TII). emerging information on not only shed light mechanisms host defense but revolutionizing long-held view vaccination vaccine strategies. Our current review will examine recent progress gaps with focus tissue-resident Mϕs, particularly lung their relationship local antimicrobial We discuss impact TII concept strategies significance respiratory mucosal route against pathogens.

Language: Английский

Citations

79
Epicutaneous immunization with modified vaccinia Ankara viral vectors generates superior T cell immunity against a respiratory viral challenge DOI Creative Commons

Youdong Pan,

Luzheng Liu, Tian Tian

et al.

npj Vaccines, Journal Year: 2021, Volume and Issue: 6(1)

Published: Jan. 4, 2021

Abstract Modified Vaccinia Ankara (MVA) was recently approved as a smallpox vaccine. Variola is transmitted by respiratory droplets and MVA immunization skin scarification (s.s.) protected mice far more effectively against lethal challenge with vaccinia virus (VACV) than any other route of delivery, at lower doses. Comparisons s.s. intradermal, subcutaneous, or intramuscular routes showed that OVA s.s.-generated T cells were both abundant transcriptionally unique. produced greater numbers lung Ova-specific CD8 + RM superior in protecting VACV challenge. Nearly many generated compared to intra-tracheal vaccination pulmonary . Strikingly, effector exhibited overlapping gene transcriptional profiles those via immunization. Overall, our data suggest heterologous vectors immunized are uniquely well-suited vaccine for pathogens, which may be relevant COVID-19. In addition, delivered could represent effective dose-sparing

Language: Английский

Citations

63