Nature Microbiology,
Journal Year:
2021,
Volume and Issue:
6(10), P. 1219 - 1232
Published: Sept. 1, 2021
The
coronavirus
disease
2019
(COVID-19)
pandemic
has
claimed
millions
of
lives
and
caused
a
global
economic
crisis.
No
effective
antiviral
drugs
are
currently
available
to
treat
infections
severe
acute
respiratory
syndrome
2
(SARS-CoV-2).
medical
need
imposed
by
the
spurred
unprecedented
research
efforts
study
biology.
Every
virus
depends
on
cellular
host
factors
pathways
for
successful
replication.
These
proviral
represent
attractive
targets
therapy
as
they
genetically
more
stable
than
viral
may
be
shared
among
related
viruses.
application
various
'omics'
technologies
led
rapid
discovery
that
required
completion
SARS-CoV-2
life
cycle.
In
this
Review,
we
summarize
insights
into
infection
were
mainly
obtained
using
functional
genetic
interactome
screens.
We
discuss
processes
important
cycle,
well
parallels
with
non-coronaviruses.
Finally,
highlight
could
targeted
clinically
approved
molecules
in
clinical
trials
potential
therapies
COVID-19.
Proviral
infection,
replication
COVID-19
reviewed.
Protein Science,
Journal Year:
2020,
Volume and Issue:
30(1), P. 187 - 200
Published: Oct. 18, 2020
The
BioGRID
(Biological
General
Repository
for
Interaction
Datasets,
thebiogrid.org)
is
an
open-access
database
resource
that
houses
manually
curated
protein
and
genetic
interactions
from
multiple
species
including
yeast,
worm,
fly,
mouse,
human.
~1.93
million
in
can
be
used
to
build
complex
networks
facilitate
biomedical
discoveries,
particularly
as
related
human
health
disease.
All
content
primary
experimental
evidence
the
literature,
includes
both
focused
low-throughput
studies
large
high-throughput
datasets.
also
captures
post-translational
modifications
or
gene
with
bioactive
small
molecules
many
known
drugs.
A
built-in
network
visualization
tool
combines
all
annotations
allows
users
generate
graphs
of
protein,
chemical
interactions.
In
addition
general
curation
across
species,
undertakes
themed
projects
specific
aspects
cellular
regulation,
example
ubiquitin-proteasome
system,
well
disease
areas,
such
SARS-CoV-2
virus
causes
COVID-19
severe
acute
respiratory
syndrome.
recent
extension
BioGRID,
named
Open
CRISPR
Screens
(ORCS,
orcs.thebiogrid.org),
single
mutant
phenotypes
published
high
throughput
genome-wide
CRISPR/Cas9-based
screens.
BioGRID-ORCS
contains
datasets
over
1,042
screens
carried
out
date
human,
mouse
fly
cell
lines.
research
community
freely
access
data
through
web
interface,
standardized
file
downloads,
via
model
organism
databases
partner
meta-databases.
Cell,
Journal Year:
2020,
Volume and Issue:
183(4), P. 968 - 981.e7
Published: Sept. 6, 2020
Summary
Severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
infection
is
typically
very
mild
and
often
asymptomatic
in
children.
A
complication
the
rare
multisystem
inflammatory
children
(MIS-C)
associated
with
COVID-19,
presenting
4–6
weeks
after
as
high
fever,
organ
dysfunction,
strongly
elevated
markers
of
inflammation.
The
pathogenesis
unclear
but
has
overlapping
features
Kawasaki
disease
suggestive
vasculitis
a
likely
autoimmune
etiology.
We
apply
systems-level
analyses
blood
immune
cells,
cytokines,
autoantibodies
healthy
children,
enrolled
prior
to
infected
SARS-CoV-2,
MIS-C.
find
that
response
MIS-C
differs
from
cytokine
storm
severe
shares
several
disease,
also
this
condition
respect
T
cell
subsets,
interleukin
(IL)-17A,
biomarkers
arterial
damage.
Finally,
autoantibody
profiling
suggests
multiple
could
be
involved
Nature Communications,
Journal Year:
2020,
Volume and Issue:
11(1)
Published: Nov. 18, 2020
Abstract
Severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2),
the
causative
agent
of
COVID19
pandemic,
is
a
highly
pathogenic
β-coronavirus.
As
other
coronaviruses,
SARS-CoV-2
enveloped,
replicates
in
cytoplasm
and
assembles
at
intracellular
membranes.
Here,
we
structurally
characterize
viral
replication
compartment
report
critical
insights
into
budding
mechanism
virus,
structure
extracellular
virions
close
to
their
native
state
by
situ
cryo-electron
tomography
subtomogram
averaging.
We
directly
visualize
RNA
filaments
inside
double
membrane
vesicles,
compartments
associated
with
replication.
The
show
diameter
consistent
double-stranded
frequent
branching
likely
representing
secondary
structures.
that
assembled
S
trimers
lumenal
cisternae
do
not
alone
induce
bending
but
laterally
reorganize
on
envelope
during
virion
assembly.
ribonucleoprotein
complexes
(vRNPs)
are
accumulated
curved
characteristic
for
sites
suggesting
vRNP
recruitment
enhanced
curvature.
Subtomogram
averaging
shows
vRNPs
distinct
cylindrical
assemblies.
propose
genome
packaged
around
multiple
separate
complexes,
thereby
allowing
incorporation
unusually
large
while
maintaining
high
steric
flexibility
between
vRNPs.
Science,
Journal Year:
2020,
Volume and Issue:
370(6521)
Published: Oct. 15, 2020
The
COVID-19
pandemic,
caused
by
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2),
is
a
grave
threat
to
public
health
and
the
global
economy.
SARS-CoV-2
closely
related
more
lethal
but
less
transmissible
coronaviruses
SARS-CoV-1
Middle
East
(MERS-CoV).
Here,
we
have
carried
out
comparative
viral-human
protein-protein
interaction
viral
protein
localization
analyses
for
all
three
viruses.
Subsequent
functional
genetic
screening
identified
host
factors
that
functionally
impinge
on
proliferation,
including
Tom70,
mitochondrial
chaperone
interacts
with
both
ORF9b,
an
structurally
characterized
using
cryo-electron
microscopy.
Combining
genetically
validated
patient
data
medical
billing
records
molecular
mechanisms
potential
drug
treatments
merit
further
clinical
study.
Signal Transduction and Targeted Therapy,
Journal Year:
2021,
Volume and Issue:
6(1)
Published: Aug. 1, 2021
Abstract
Emerging
evidence
suggests
that
liquid–liquid
phase
separation
(LLPS)
represents
a
vital
and
ubiquitous
phenomenon
underlying
the
formation
of
membraneless
organelles
in
eukaryotic
cells
(also
known
as
biomolecular
condensates
or
droplets).
Recent
studies
have
revealed
evidences
indicate
LLPS
plays
role
human
health
diseases.
In
this
review,
we
describe
our
current
understanding
summarize
its
physiological
functions.
We
further
development
Additionally,
review
recently
developed
methods
for
studying
LLPS.
Although
research
is
infancy—but
fast-growing—it
clear
an
essential
pathophysiological
conditions.
This
highlights
need
overview
recent
advances
field
to
translate
knowledge
regarding
into
therapeutic
discoveries.
