Nature,
Journal Year:
2023,
Volume and Issue:
618(7964), P. 349 - 357
Published: May 31, 2023
The
incidence
of
Alzheimer's
disease
(AD),
the
leading
cause
dementia,
increases
rapidly
with
age,
but
why
age
constitutes
main
risk
factor
is
still
poorly
understood.
Brain
ageing
affects
oligodendrocytes
and
structural
integrity
myelin
sheaths1,
latter
which
associated
secondary
neuroinflammation2,3.
As
support
axonal
energy
metabolism
neuronal
health4-7,
we
hypothesized
that
loss
could
be
an
upstream
for
amyloid-β
(Aβ)
deposition,
central
neuropathological
hallmark
AD.
Here
identify
genetic
pathways
dysfunction
demyelinating
injuries
as
potent
drivers
amyloid
deposition
in
mouse
models
Mechanistically,
causes
accumulation
Aβ-producing
machinery
within
swellings
cleavage
cortical
precursor
protein.
Suprisingly,
AD
mice
dysfunctional
lack
plaque-corralling
microglia
despite
overall
increase
their
numbers.
Bulk
single-cell
transcriptomics
defects
show
there
a
concomitant
induction
highly
similar
distinct
disease-associated
signatures
specific
to
damage
plaques,
respectively.
Despite
successful
induction,
(DAM)
usually
clear
plaques
are
apparently
distracted
nearby
damage.
Our
data
suggest
working
model
whereby
age-dependent
promote
Aβ
plaque
formation
directly
indirectly
therefore
factor.
Improving
oligodendrocyte
health
promising
target
delay
development
slow
progression
Genome Medicine,
Journal Year:
2022,
Volume and Issue:
14(1)
Published: June 27, 2022
Abstract
Single-cell
transcriptomics
(scRNA-seq)
has
become
essential
for
biomedical
research
over
the
past
decade,
particularly
in
developmental
biology,
cancer,
immunology,
and
neuroscience.
Most
commercially
available
scRNA-seq
protocols
require
cells
to
be
recovered
intact
viable
from
tissue.
This
precluded
many
cell
types
study
largely
destroys
spatial
context
that
could
otherwise
inform
analyses
of
identity
function.
An
increasing
number
platforms
now
facilitate
spatially
resolved,
high-dimensional
assessment
gene
transcription,
known
as
‘spatial
transcriptomics’.
Here,
we
introduce
different
classes
method,
which
either
record
locations
hybridized
mRNA
molecules
tissue,
image
positions
themselves
prior
assessment,
or
employ
arrays
probes
pre-determined
location.
We
review
sizes
tissue
area
can
assessed,
their
resolution,
genes
profiled.
discuss
if
preservation
influences
choice
platform,
provide
guidance
on
whether
specific
may
better
suited
discovery
screens
hypothesis
testing.
Finally,
bioinformatic
methods
analysing
transcriptomic
data,
including
pre-processing,
integration
with
existing
inference
cell-cell
interactions.
Spatial
-omics
are
already
improving
our
understanding
human
tissues
research,
diagnostic,
therapeutic
settings.
To
build
upon
these
recent
advancements,
entry-level
those
seeking
own
research.
Journal of Neuroinflammation,
Journal Year:
2022,
Volume and Issue:
19(1)
Published: June 6, 2022
Abstract
Major
depressive
disorder
is
a
highly
debilitating
psychiatric
involving
the
dysfunction
of
different
cell
types
in
brain.
Microglia
are
predominant
resident
immune
cells
brain
and
exhibit
critical
role
depression.
Recent
studies
have
suggested
that
depression
can
be
regarded
as
microglial
disease.
regulate
inflammation,
synaptic
plasticity,
formation
neural
networks,
all
which
affect
In
this
review,
we
highlighted
microglia
pathology
First,
described
activation
animal
models
clinically
depressed
patients.
Second,
emphasized
possible
mechanisms
by
recognize
depression-associated
stress
conditions.
Third,
how
antidepressants
(clinical
medicines
natural
products)
activation.
Thus,
review
aimed
to
objectively
analyze
focus
on
potential
antidepressants.
These
data
regulation
actions
might
novel
therapeutic
strategy
counteract
adverse
effects
devastating
mental
disorders.
