Signal Transduction and Targeted Therapy,
Journal Year:
2024,
Volume and Issue:
9(1)
Published: Feb. 26, 2024
Precise
genome-editing
platforms
are
versatile
tools
for
generating
specific,
site-directed
DNA
insertions,
deletions,
and
substitutions.
The
continuous
enhancement
of
these
has
led
to
a
revolution
in
the
life
sciences,
which
promises
deliver
novel
therapies
genetic
disease.
can
be
traced
back
1950s
with
discovery
DNA's
double-helix
and,
after
70
years
development,
evolved
from
crude
vitro
applications
wide
range
sophisticated
capabilities,
including
vivo
applications.
Nonetheless,
precise
faces
constraints
such
as
modest
efficiency,
delivery
challenges,
off-target
effects.
In
this
review,
we
explore
genome-editing,
focus
on
introduction
landmark
events
its
history,
various
platforms,
systems,
First,
discuss
history
genome-editing.
Second,
describe
current
state
strategies
explain
how
techniques
offer
unprecedented
precision
versatility
modifying
human
genome.
Third,
introduce
systems
used
deploy
components
through
DNA,
RNA,
RNPs.
Finally,
summarize
labeling
endogenous
genes,
screening
variants,
molecular
recording,
disease
models,
gene
therapy,
ex
therapy
potential
future
advances.
Science,
Journal Year:
2023,
Volume and Issue:
379(6629)
Published: Jan. 19, 2023
The
advent
of
clustered
regularly
interspaced
short
palindromic
repeat
(CRISPR)
genome
editing,
coupled
with
advances
in
computing
and
imaging
capabilities,
has
initiated
a
new
era
which
genetic
diseases
individual
disease
susceptibilities
are
both
predictable
actionable.
Likewise,
genes
responsible
for
plant
traits
can
be
identified
altered
quickly,
transforming
the
pace
agricultural
research
breeding.
In
this
Review,
we
discuss
current
state
CRISPR-mediated
manipulation
human
cells,
animals,
plants
along
relevant
successes
challenges
present
roadmap
future
technology.
