The LRR receptor-like kinase ALR1 is a plant aluminum ion sensor

Cell Research, Journal Year: 2024, Volume and Issue: 34(4), P. 281 - 294

Published: Jan. 10, 2024

Abstract Plant survival requires an ability to adapt differing concentrations of nutrient and toxic soil ions, yet ion sensors associated signaling pathways are mostly unknown. Aluminum (Al) ions highly phytotoxic, cause severe crop yield loss forest decline on acidic soils which represent ∼30% land areas worldwide. Here we found Arabidopsis mutant hypersensitive Al. The gene encoding a leucine-rich-repeat receptor-like kinase, was named Al Resistance1 (ALR1). binding ALR1 cytoplasmic domain recruits BAK1 co-receptor kinase promotes ALR1-dependent phosphorylation the NADPH oxidase RbohD, thereby enhancing reactive oxygen species (ROS) generation. ROS in turn oxidatively modify RAE1 F-box protein inhibit RAE1-dependent proteolysis central regulator STOP1, thus activating organic acid anion secretion detoxify These findings establish as receptor that confers resistance through integrated Al-triggered pathway, providing novel insights into ion-sensing mechanisms living organisms, enabling future molecular breeding acid-soil-tolerant crops trees, with huge potential for both global food security restoration.

Language: Английский

---

Biomolecular condensation orchestrates clathrin-mediated endocytosis in plants

Nature Cell Biology, Journal Year: 2024, Volume and Issue: 26(3), P. 438 - 449

Published: Feb. 12, 2024

Language: Английский

---

Direct observation of translational activation by a ribonucleoprotein granule

Nature Cell Biology, Journal Year: 2024, Volume and Issue: 26(8), P. 1322 - 1335

Published: July 4, 2024

Biomolecular condensates organize biochemical processes at the subcellular level and can provide spatiotemporal regulation within a cell. Among these, ribonucleoprotein (RNP) granules are storage hubs for translationally repressed mRNA. Whether RNP also activate translation how this could be achieved remains unclear. Here, using single-molecule imaging, we demonstrate that germ cell-determining in Drosophila embryos sites active of nanos Nanos occurs preferentially granule surface with 3' UTR buried granule. Smaug, cytosolic RNA-binding protein, represses translation, which is relieved when Smaug sequestered to by scaffold protein Oskar. Together, our findings uncover molecular process achieve localized synthesis through compartmentalized loss translational repression.

Language: Английский

---

Molecular determinants of condensate composition

Molecular Cell, Journal Year: 2025, Volume and Issue: 85(2), P. 290 - 308

Published: Jan. 1, 2025

Language: Английский

---

Clustering of Aromatic Residues in Prion-like Domains Can Tune the Formation, State, and Organization of Biomolecular Condensates

Biochemistry, Journal Year: 2021, Volume and Issue: 60(47), P. 3566 - 3581

Published: Nov. 16, 2021

In immature oocytes, Balbiani bodies are conserved membraneless condensates implicated in oocyte polarization, the organization of mitochondria, and long-term organelle RNA storage. Xenopus laevis, body assembly is mediated by protein Velo1. Velo1 contains an N-terminal prion-like domain (PLD) that essential for formation. PLDs have emerged as a class intrinsically disordered regions can undergo various different types intracellular phase transitions often associated with dynamic, liquid-like condensates. Intriguingly, PLD forms solid-like assemblies. Here we sought to understand why behavior appears be biophysically distinct from other PLD-containing proteins. Through bioinformatic analysis coarse-grained simulations, predict clustering aromatic residues amino acid composition between aromatics influence condensate material properties, organization, driving forces assembly. To test our predictions, redesigned impact targeted sequence changes vivo. We found design evenly spaced shows rapid internal dynamics, probed fluorescent recovery after photobleaching, even when recruited into bodies. Our results suggest might been selected evolution distinctly clustered maintain structure long-lived oocytes. general, work identifies several tunable parameters used augment state, offering road map synthetic

Language: Английский

---

Condensation of SEUSS promotes hyperosmotic stress tolerance in Arabidopsis

Nature Chemical Biology, Journal Year: 2022, Volume and Issue: 18(12), P. 1361 - 1369

Published: Nov. 14, 2022

Language: Английский

---

Plants use molecular mechanisms mediated by biomolecular condensates to integrate environmental cues with development

The Plant Cell, Journal Year: 2023, Volume and Issue: 35(9), P. 3173 - 3186

Published: March 5, 2023

Abstract This review highlights recent literature on biomolecular condensates in plant development and discusses challenges for fully dissecting their functional roles. Plant developmental biology has been inundated with descriptive examples of condensate formation, but it is only recently that mechanistic understanding forthcoming. Here, we discuss potential roles play at different stages the life cycle. We group these based putative molecular functions, including sequestering interacting components, enhancing dwell time, cytoplasmic biophysical properties response to environmental change. explore how mechanisms could modulate inputs opportunities further research into deciphering better understand diverse exert life.

Language: Английский

---

Phase separation of SGS3 drives siRNA body formation and promotes endogenous gene silencing

Cell Reports, Journal Year: 2023, Volume and Issue: 42(1), P. 111985 - 111985

Published: Jan. 1, 2023

The generation of small interfering RNA (siRNA) involves many processing components, including SUPPRESSOR OF GENE SILENCING 3 (SGS3), RNA-DEPENDENT POLYMERASE 6 (RDR6), and DICER-LIKE proteins (DCLs). Nonetheless, how these components are coordinated to produce siRNAs is unclear. Here, we show that SGS3 forms condensates via phase separation in vivo vitro. interacts with RDR6 drives it form siRNA bodies cytoplasm, which promoted by SGS3-targeted RNAs. Disrupting abrogates body assembly biogenesis, whereas coexpression induces formation tobacco yeast cells. Dysfunction translation mRNA decay increases the number bodies, DCL2/4 mutations enhance their size. Purification identifies numerous RNA-binding components. Together, our findings reveal separation-mediated essential for production gene silencing.

Language: Английский

---

Phase separation and molecular ordering of the prion-like domain of the Arabidopsis thermosensory protein EARLY FLOWERING 3

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(28)

Published: July 3, 2023

Liquid-liquid phase separation (LLPS) is an important mechanism enabling the dynamic compartmentalization of macromolecules, including complex polymers such as proteins and nucleic acids, occurs a function physicochemical environment. In model plant,

Language: Английский

---

Sodium ion influx regulates liquidity of biomolecular condensates in hyperosmotic stress response

Cell Reports, Journal Year: 2023, Volume and Issue: 42(4), P. 112315 - 112315

Published: April 1, 2023

Biomolecular condensates are membraneless structures formed through phase separation. Recent studies have demonstrated that the material properties of biomolecular crucial for their biological functions and pathogenicity. However, maintenance in cells remains elusive. Here, we show sodium ion (Na+) influx regulates condensate liquidity under hyperosmotic stress. ASK3 higher fluidity at high intracellular Na+ concentration derived from extracellular solution. Moreover, identified TRPM4 as a cation channel allows inhibition causes liquid-to-solid transition condensates, leading to impairment osmoresponse. In addition widely aggregate formation biomolecules, including DCP1A, TAZ, polyQ-protein, Our findings demonstrate changes contribute cellular stress response via condensates.

Language: Английский

---