bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: March 25, 2024
The
rise
of
large
scientific
collaborations
in
neuroscience
requires
systematic,
scalable,
and
reliable
data
management.
How
this
is
best
done
practice
remains
an
open
question.
To
address
this,
we
conducted
a
science
survey
among
currently
active
U19
grants,
funded
through
the
NIH’s
BRAIN
Initiative.
was
answered
by
both
liaisons
Principal
Investigators,
speaking
for
∼500
researchers
across
21
nation-wide
collaborations.
We
describe
tools,
technologies,
methods
use,
identify
several
shortcomings
current
practice.
Building
on
survey,
develop
plans
propose
policies
to
improve
collection,
publication,
re-use
training
community.
Journal of Pathology and Translational Medicine,
Journal Year:
2023,
Volume and Issue:
57(1), P. 52 - 59
Published: Jan. 10, 2023
Single-cell
RNA
sequencing
has
become
a
powerful
and
essential
tool
for
delineating
cellular
diversity
in
normal
tissues
alterations
disease
states.
For
certain
cell
types
conditions,
there
are
difficulties
isolating
intact
cells
transcriptome
profiling
due
to
their
fragility,
large
size,
tight
interconnections,
other
factors.
Single-nucleus
(snRNA-seq)
is
an
alternative
or
complementary
approach
that
difficult
isolate.
In
this
review,
we
will
provide
overview
of
the
experimental
analysis
steps
snRNA-seq
understand
methods
characteristics
general
tissue-specific
data.
Knowing
advantages
limitations
increase
its
use
improve
biological
interpretation
data
generated
using
technique.
Journal Of Big Data,
Journal Year:
2023,
Volume and Issue:
10(1)
Published: July 10, 2023
Neurological
diseases
are
on
the
rise
worldwide,
leading
to
increased
healthcare
costs
and
diminished
quality
of
life
in
patients.
In
recent
years,
Big
Data
has
started
transform
fields
Neuroscience
Neurology.
Scientists
clinicians
collaborating
global
alliances,
combining
diverse
datasets
a
massive
scale,
solving
complex
computational
problems
that
demand
utilization
increasingly
powerful
resources.
This
revolution
is
opening
new
avenues
for
developing
innovative
treatments
neurological
diseases.
Our
paper
surveys
Data's
impact
patient
care,
as
exemplified
through
work
done
comprehensive
selection
areas,
including
Connectomics,
Alzheimer's
Disease,
Stroke,
Depression,
Parkinson's
Pain,
Addiction
(e.g.,
Opioid
Use
Disorder).
We
present
an
overview
research
methodologies
utilizing
each
area,
well
their
current
limitations
technical
challenges.
Despite
potential
benefits,
full
these
currently
remains
unrealized.
close
with
recommendations
future
aimed
at
optimizing
use
Neurology
improved
outcomes.The
online
version
contains
supplementary
material
available
10.1186/s40537-023-00751-2.
Drosophila
melanogaster
reproductive
behaviors
are
orchestrated
by
fruitless
neurons.
We
performed
single-cell
RNA-sequencing
on
pupal
neurons
that
produce
sex-specifically
spliced
fru
transcripts,
the
P1-expressing
Uniform
Manifold
Approximation
and
Projection
(UMAP)
with
clustering
generates
an
atlas
containing
113
clusters.
While
male
female
overlap
in
UMAP
space,
more
than
half
clusters
have
sex
differences
neuron
number,
nearly
all
display
sex-differential
expression.
Based
examination
of
enriched
marker
genes,
we
annotate
as
circadian
clock
neurons,
mushroom
body
Kenyon
cell
neurotransmitter-
and/or
neuropeptide-producing,
those
express
doublesex
.
Marker
gene
analyses
also
show
genes
encode
members
immunoglobulin
superfamily
adhesion
molecules,
transcription
factors,
neuropeptides,
neuropeptide
receptors,
Wnts
unique
patterns
expression
across
In
vivo
spatial
links
to
examined.
A
functional
analysis
P1
shows
they
dimorphic
roles
activity
period
length.
Given
most
comprised
indicates
sexes
common
programs.
Sex-specific
is
overlaid
this
program,
build
potential
for
vastly
different
sex-specific
behaviors.
Molecular Psychiatry,
Journal Year:
2023,
Volume and Issue:
28(10), P. 4407 - 4420
Published: March 23, 2023
The
study
of
Alzheimer's
Disease
(AD)
has
traditionally
focused
on
neuropathological
mechanisms
that
guided
therapies
attenuate
features.
A
new
direction
is
emerging
in
AD
research
focuses
the
progressive
loss
cognitive
function
due
to
disrupted
neural
circuit
mechanisms.
Evidence
from
humans
and
animal
models
show
dysregulated
circuits
initiate
a
cascade
pathological
events
culminate
functional
learning,
memory,
other
aspects
cognition.
Recent
progress
single-cell,
spatial,
omics
informs
this
circuit-focused
approach
by
determining
identities,
locations,
circuitry
specific
cells
affected
AD.
Recently
developed
neuroscience
tools
allow
for
precise
access
cell
type-specific
so
their
roles
AD-related
deficits
disease
progression
can
be
tested.
An
integrated
systems-level
understanding
AD-associated
requires
multimodal
multi-scale
interrogations
longitudinally
measure
and/or
manipulate
ensemble
properties
molecularly-defined
neuron
populations
first
susceptible
These
newly
technological
conceptual
advances
present
opportunities
studying
treating
vulnerable
represent
beginning
era
circuit-based
research.
CNS Spectrums,
Journal Year:
2023,
Volume and Issue:
29(1), P. 26 - 39
Published: Sept. 7, 2023
Abstract
Psychiatric
disorders
are
associated
with
significant
social
and
economic
burdens,
many
of
which
related
to
issues
current
diagnosis
treatments.
The
coronavirus
(COVID-19)
pandemic
is
estimated
have
increased
the
prevalence
burden
major
depressive
anxiety
disorders,
indicating
an
urgent
need
strengthen
mental
health
systems
globally.
To
date,
approaches
adopted
in
drug
discovery
development
for
psychiatric
been
relatively
unsuccessful.
Precision
psychiatry
aims
tailor
healthcare
more
closely
needs
individual
patients
and,
when
informed
by
neuroscience,
can
offer
opportunity
improve
accuracy
disease
classification,
treatment
decisions,
prevention
efforts.
In
this
review,
we
highlight
growing
global
interest
precision
potential
National
Institute
Health-devised
Research
Domain
Criteria
(RDoC)
facilitate
implementation
transdiagnostic
improved
approaches.
nosology
evolve
recent
scientific
advancements
increase
awareness
emerging
investigators/clinicians
value
approach
essential.
Finally,
examine
challenges
future
opportunities
adopting
RDoC-associated
translational
clinical
studies,
acknowledging
that
strength
RDoC
they
form
a
dynamic
framework
guiding
principles
intended
continuously
developments
into
future.
A
collaborative
recruits
expertise
from
multiple
disciplines,
while
also
considering
patient
perspective,
needed
pave
way
prognosis
quality
life
patients.
Nature Communications,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: Jan. 9, 2024
Abstract
Invariant
cell
lineage
in
C.
elegans
enables
spatiotemporal
resolution
of
transcriptional
regulatory
mechanisms
controlling
the
fate
each
cell.
Here,
we
develop
RAPCAT
(Robust-point-matching-
And
Piecewise-affine-based
Cell
Annotation
Tool)
to
automate
identity
assignment
three-dimensional
image
stacks
L1
larvae
and
profile
reporter
expression
620
transcription
factors
every
Transcription
factor
profile-based
clustering
analysis
defines
80
types
distinct
from
conventional
phenotypic
identifies
three
general
modalities
related
these
classifications.
First,
are
broadly
downregulated
quiescent
stage
Hermaphrodite
Specific
Neurons,
suggesting
stage-
type-specific
variation
transcriptome
size.
Second,
is
more
closely
associated
with
morphology
than
other
different
pre-
post-differentiation
developmental
stages.
Finally,
embryonic
lineages
can
be
specific
patterns
functions
that
persist
throughout
postembryonic
life.
This
study
presents
a
comprehensive
atlas
for
investigation
intra-cell
type
heterogeneity.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2022,
Volume and Issue:
unknown
Published: Jan. 9, 2022
Abstract
Adeno-associated
viruses
(AAVs)
can
enable
robust
and
safe
gene
delivery
to
the
mammalian
central
nervous
system
(CNS).
While
scientific
community
has
developed
numerous
neurotropic
AAV
variants
for
systemic
gene-transfer
rodent
brain,
there
are
few
AAVs
that
efficiently
access
CNS
of
higher
order
primates.
We
describe
here
AAV.CAP-Mac,
an
engineered
variant
enables
systemic,
brain-wide
in
infants
two
Old
World
primate
species—the
rhesus
macaque
(
Macaca
mulatta
)
green
monkey
Chlorocebus
sabaeus
).
identified
CAP-Mac
using
a
multi-species
selection
strategy,
initially
screening
our
library
adult
common
marmoset
Callithrix
jacchus
narrowing
pool
test-variants
another
round
infant
macaques.
In
individual
characterization,
robustly
transduces
human
neurons
vitro
vivo
,
where
it
targets
all
lobes
cortex,
cerebellum,
multiple
subcortical
regions
disease
relevance.
use
Brainbow-like
multicolor
labeling
throughout
enabling
morphological
reconstruction
both
medium
spiny
cortical
pyramidal
cells.
Because
its
broad
distribution
brain
high
neuronal
efficiency
primates
compared
AAV9,
shows
promise
researchers
clinicians
alike
unlock
novel,
noninvasive
efficient
transfer.