Long journey of 16S rRNA‐amplicon sequencing toward cell‐based functional bacterial microbiota characterization

iMetaOmics., Journal Year: 2024, Volume and Issue: 1(1)

Published: July 11, 2024

Abstract Bacteria often exist and function as a community, known the bacterial microbiota, which consists of vast numbers bacteria belonging to many species (taxa). Characterizing microbiota needs high‐throughput approaches that enable identification quantification cells, such have been under development for more than 30 years. In this review, we describe history technologies based on 16S ribosomal RNA (rRNA) gene‐amplicon sequencing characterization microbiotas. Then, summarize features applications current rRNA approaches, including recent achievement enables individual cells with single‐base accuracy genes identified cells. Furthermore, present prospects further technical development, combined use methods other informative analyses, whole‐genome in common unit cell, both number their functions.

Language: Английский

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The gut microbiome dysbiosis and regulation by fecal microbiota transplantation: umbrella review

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Nov. 3, 2023

Background Gut microbiome dysbiosis has been implicated in various gastrointestinal and extra-gastrointestinal diseases, but evidence on the efficacy safety of fecal microbiota transplantation (FMT) for therapeutic indications remains unclear. Methods The gutMDisorder database was used to summarize associations between gut diseases. We performed an umbrella review published meta-analyses determine synthesis FMT treating Our study registered PROSPERO (CRD42022301226). Results associated with 117 extra-gastrointestinal. Colorectal cancer 92 dysbiosis. Dysbiosis involving Firmicutes (phylum) 34 identified 62 FMT. found be effective 13 a 95.56% cure rate (95% CI: 93.88–97.05%) recurrent Chloridoids difficile infection (rCDI). Evidence high quality rCDI moderate ulcerative colitis Crohn’s disease low very other Conclusion may numerous Substantial suggests improves clinical outcomes certain indications, varies greatly depending specific indication, route administration, frequency instillation, preparation, donor type. This variability should inform clinical, policy, implementation decisions regarding

Language: Английский

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Intratumour microbiome of pancreatic cancer

World Journal of Gastrointestinal Oncology, Journal Year: 2023, Volume and Issue: 15(5), P. 713 - 730

Published: May 11, 2023

Pancreatic cancer is a high mortality malignancy with almost equal and morbidity rates. Both normal tumour tissues of the pancreas were previously considered sterile. In recent years, development technologies for high-throughput sequencing, variety studies have revealed that pancreatic contain small amounts bacteria fungi. The intratumour microbiome being as an influential contributor to carcinogenesis. has been identified crucial factor progression, diagnosis, treatment, chemotherapy resistance, immune response. A better understanding biology contributes establishment early screening treatment strategies. This review focuses on possible origins in cancer, localization, interaction microenvironment, strategies improving outcome treatment. Thus, this offers new perspectives prognosis cancer.

Language: Английский

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Taking Microbiome Science to the Next Level: Recommendations to Advance the Emerging Field of Microbiome-Based Therapeutics and Diagnostics

Gastroenterology, Journal Year: 2024, Volume and Issue: 167(6), P. 1059 - 1064

Published: May 28, 2024

Language: Английский

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High-throughput identification and quantification of bacterial cells in the microbiota based on 16S rRNA sequencing with single-base accuracy using BarBIQ

Nature Protocols, Journal Year: 2023, Volume and Issue: 19(1), P. 207 - 239

Published: Nov. 27, 2023

Language: Английский

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