Biosensors,
Journal Year:
2025,
Volume and Issue:
15(3), P. 162 - 162
Published: March 3, 2025
The
accurate
detection
of
Mycobacterium
tuberculosis
(MTB)
is
a
pressing
challenge
in
the
precise
prevention
and
control
tuberculosis.
Currently,
efficiency
accuracy
drug
resistance
for
MTB
are
low,
cross-contamination
common,
making
it
inadequate
clinical
needs.
This
study
developed
rapid
nucleic
acid
method
based
on
scattering
loop-mediated
isothermal
amplification
(LAMP).
Specific
primers
MTB-specific
gene
(Ag85B)
were
designed,
LAMP
reaction
system
was
optimized
using
self-developed
turbidimeter.
Experimental
results
showed
that
optimal
included
1.5
µL
100
mmol/L
magnesium
ions,
3.5
10
dNTPs,
6
1.6
mol/L
betaine,
temperature
65
°C.
minimum
limit
12.40
ng/L,
with
fastest
time
being
approximately
min.
exhibited
good
specificity,
no
bands
other
pathogens.
Twenty
culture-positive
samples
twenty
culture-negative
tested
parallel;
positive
group
100%,
(24.9
±
13
min),
there
negative
detection.
features
high
efficiency,
low
cost,
accuracy,
effectively
reduces
cross-contamination,
providing
new
technology
MTB.
MedComm,
Journal Year:
2025,
Volume and Issue:
6(4)
Published: April 1, 2025
ABSTRACT
Tuberculous
pleural
effusion
(TPE)
is
a
prevalent
form
of
extrapulmonary
tuberculosis,
and
immune
abnormalities
play
crucial
role
in
promoting
its
development.
However,
the
dynamic
changes
regulatory
characteristics
cells
during
TPE
progression
remain
incompletely
understood.
This
study
analyzed
DNA
methylation
transcriptome
data
from
macrophages
CD4
+
T
lavage
fluid
BCG‐induced
tuberculous
pleurisy
mouse
models
at
specific
time
points
(Days
0,
1,
7,
14).
The
results
revealed
substantial
alterations
patterns
associated
with
inflammatory
factors
interferon
genes.
Notably,
exhibited
most
pronounced
differences
profiles
on
Day
while
demonstrated
gradual
over
time.
investigation
further
indicated
that
primarily
regulated
differentiation
Th1,
Th17,
Th22
but
not
Th9
cells.
Additionally,
single‐cell
RNA
sequencing
analysis
an
increasing
expression
C1q
infection,
which
was
by
methylation.
Importantly,
−
distinct
roles
modulating
responses
infection.
research
provides
valuable
insights
into
profile
Mycobacterium
bovis
infection–induced
model,
enhancing
our
understanding
upstream
mechanisms
underlying
response
development
TPE.
Infection and Immunity,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 4, 2025
ABSTRACT
Tuberculosis
(TB)
is
notoriously
difficult
to
treat,
likely
due
the
complex
host-pathogen
interactions
driven
by
pathogen
heterogeneity.
An
understudied
area
of
TB
pathogenesis
host
responses
Mycobacterium
tuberculosis
bacteria
(Mtb)
that
are
limited
in
zinc
ions.
This
distinct
population
resides
necrotic
granulomas
and
sputum
could
be
key
player
pathogenicity.
In
this
study,
we
tested
hypothesis
macrophages
differentiate
between
Mtb
grown
under
limitation
or
standard
zinc-replete
medium.
Using
several
macrophage
infection
models,
such
as
murine
RAW
264.7
bone
marrow-derived
(BMDMs),
well
human
THP-1-derived
macrophages,
show
infected
with
zinc-limited
have
increased
bacterial
burden
compared
Mtb.
We
further
demonstrate
trigger
higher
production
reactive
oxygen
species
(ROS)
cause
more
death.
Furthermore,
ROS
linked
phagocytosis
Mtb,
whereas
cell
death
not.
Finally,
transcriptional
analysis
demonstrates
robust
pro-inflammatory
when
than
Together,
our
findings
suggest
Mtb’s
access
affects
their
interaction
may
influencing
progression.
Therefore,
availability
growth
medium
should
considered
drug
vaccine
developments.
Biosensors,
Journal Year:
2025,
Volume and Issue:
15(3), P. 162 - 162
Published: March 3, 2025
The
accurate
detection
of
Mycobacterium
tuberculosis
(MTB)
is
a
pressing
challenge
in
the
precise
prevention
and
control
tuberculosis.
Currently,
efficiency
accuracy
drug
resistance
for
MTB
are
low,
cross-contamination
common,
making
it
inadequate
clinical
needs.
This
study
developed
rapid
nucleic
acid
method
based
on
scattering
loop-mediated
isothermal
amplification
(LAMP).
Specific
primers
MTB-specific
gene
(Ag85B)
were
designed,
LAMP
reaction
system
was
optimized
using
self-developed
turbidimeter.
Experimental
results
showed
that
optimal
included
1.5
µL
100
mmol/L
magnesium
ions,
3.5
10
dNTPs,
6
1.6
mol/L
betaine,
temperature
65
°C.
minimum
limit
12.40
ng/L,
with
fastest
time
being
approximately
min.
exhibited
good
specificity,
no
bands
other
pathogens.
Twenty
culture-positive
samples
twenty
culture-negative
tested
parallel;
positive
group
100%,
(24.9
±
13
min),
there
negative
detection.
features
high
efficiency,
low
cost,
accuracy,
effectively
reduces
cross-contamination,
providing
new
technology
MTB.
