Isolation and functional characterization of cold-induced gene (AmCIP) promoter from Ammopiptanthus mongolicus DOI
Bohao Ge,

Kuo Dong,

Rongchen Li

et al.

Gene, Journal Year: 2024, Volume and Issue: 909, P. 148311 - 148311

Published: Feb. 22, 2024

Language: Английский

Neuronal RNA processing: cross-talk between transcriptional regulation and RNA-binding proteins DOI Creative Commons
Hasan Can Ozbulut, Valérie Hilgers

Frontiers in Molecular Neuroscience, Journal Year: 2024, Volume and Issue: 17

Published: Aug. 1, 2024

In the nervous system, alternative RNA processing is particularly prevalent, which results in expression of thousands transcript variants found no other tissue. Neuron-specific RNA-binding proteins co-transcriptionally regulate splicing, polyadenylation, and editing, thereby shaping identity system cells. Recent evidence suggests that interactions between cis-regulatory elements such as promoters enhancers play a role determination neuron-specific profiles. Here, we discuss possible mechanisms through transcription cross-talk to generate uniquely complex neuronal transcriptome, with focus on 3′-end formation.

Language: Английский

Citations

1
The promoter as a trip navigator: Guiding alternative polyadenylation site destinations DOI Creative Commons

Alexandra Bergfort,

Karla M. Neugebauer

Molecular Cell, Journal Year: 2023, Volume and Issue: 83(14), P. 2395 - 2397

Published: July 1, 2023

Language: Английский

Citations

3
Pumilio differentially binds to mRNA 3' UTR isoforms to regulate localization of synaptic proteins DOI Creative Commons
Dominika Grzejda, Andreas Heß,

Andrew Rezansoff

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 19, 2024

Abstract In neuronal cells, the regulation of RNA is crucial for spatiotemporal control gene expression, but how correct localization, levels, and function synaptic proteins are achieved not well understood. this study, we globally investigate role alternative 3’ UTRs in regulating localization regions Drosophila brain. We identify direct mRNA targets translational repressor Pumilio, finding that mRNAs bound by Pumilio encode enriched synaptosomes. differentially binds to isoforms same gene, favoring long, UTRs. These longer tend remain soma, whereas shorter UTR localize synapse. cultured pumilio mutant neurons, severe axon outgrowth defects were accompanied isoform mislocalization, encoded these displayed excessive abundance at boutons. Our study identifies an important widespread mechanism protein neurons.

Language: Английский

Citations

0
Identification of regulatory links between transcription and RNA processing with long-read sequencing DOI Creative Commons

Carlos Alfonso-Gonzalez,

Laura Arrigoni, Hasan Can Ozbulut

et al.

STAR Protocols, Journal Year: 2023, Volume and Issue: 4(4), P. 102505 - 102505

Published: Sept. 20, 2023

We present a detailed protocol for sequencing full-length mRNA isoforms using the Oxford nanopore long-read technology. describe steps poly(A) RNA isolation, library preparation, and cDNA size selection. then detail procedures processing computational framework to identify exon couplings assign 5' ends 3' each other. Our approach enables identification of links between transcription initiation co-transcriptional events. For complete details on use execution this protocol, please refer Alfonso-Gonzalez et al.

Language: Английский

Citations

1
Isolation and functional characterization of cold-induced gene (AmCIP) promoter from Ammopiptanthus mongolicus DOI
Bohao Ge,

Kuo Dong,

Rongchen Li

et al.

Gene, Journal Year: 2024, Volume and Issue: 909, P. 148311 - 148311

Published: Feb. 22, 2024

Language: Английский

Citations

0