Na+ Binding and Transport: Insights from Light-Driven Na+-Pumping Rhodopsin DOI Creative Commons
Qifan Yang, Deliang Chen

Molecules, Journal Year: 2023, Volume and Issue: 28(20), P. 7135 - 7135

Published: Oct. 17, 2023

Na+ plays a vital role in numerous physiological processes across humans and animals, necessitating comprehensive understanding of transmembrane transport. Among the various pumps channels, light-driven Na+-pumping rhodopsin (NaR) has emerged as noteworthy model this field. This review offers concise overview structural functional studies conducted on NaR, encompassing ground/intermediate-state structures photocycle kinetics. The primary focus lies addressing key inquiries: (1) unraveling translocation pathway Na+; (2) examining changes within photocycle, particularly O state, facilitating transport; (3) investigating timing uptake/release. By delving into these unresolved issues existing debates, aims to shed light future direction pump research.

Language: Английский

Structural basis for ion selectivity in potassium-selective channelrhodopsins DOI Creative Commons
Seiya Tajima, Yoon Seok Kim, Masahiro Fukuda

et al.

Cell, Journal Year: 2023, Volume and Issue: 186(20), P. 4325 - 4344.e26

Published: Aug. 30, 2023

KCR channelrhodopsins (K

Citations

32
Light-gated channelrhodopsin sparks proton-induced calcium release in guard cells DOI
Shouguang Huang, Like Shen, M. Rob G. Roelfsema

et al.

Science, Journal Year: 2023, Volume and Issue: 382(6676), P. 1314 - 1318

Published: Dec. 14, 2023

Although there has been long-standing recognition that stimuli-induced cytosolic pH alterations coincide with changes in calcium ion (Ca2+) levels, the interdependence between protons (H+) and Ca2+ remains poorly understood. We addressed this topic using light-gated channelrhodopsin HcKCR2 from pseudofungus Hyphochytrium catenoides, which operates as a H+ conductive, impermeable channel on plasma membrane of plant cells. Light activation Arabidopsis guard cells evokes transient cytoplasmic acidification sparks release endoplasmic reticulum. A H+-induced signal results depolarization through Ca2+-dependent SLAC1/SLAH3 anion channels, enabled us to remotely control stomatal movement. Our study suggests mechanism establishes tool dissect molecular basis intracellular signaling.

Language: Английский

Citations

27
Decoupled Ionic and Electronic Pathways for Enhanced Osmotic Energy Harvesting DOI

Zhijiang Xie,

Zhongrun Xiang,

Xiaotong Fu

et al.

ACS Energy Letters, Journal Year: 2024, Volume and Issue: 9(5), P. 2092 - 2100

Published: April 24, 2024

Methods of reducing nanofluids' internal resistance by mixing conductive nanomaterials will negatively affect the nanochannel structures and ion transmissions. Herein, a layered-structured nanofluidic membrane that achieves transport in cellulose nanochannels realizes electron external polyaniline network is developed. Results show ionic conductivity resistivity layered at low salt concentrations are 1.57 times higher 0.99 lower than those blend membrane, demonstrating positive contribution decoupled electronic pathways. Furthermore, attained an enhanced output power density 11.7 W m–2 maintained performance up to 10.9 after 16 days operation under neutral 50-fold salinity concentration gradient, which commercial system (5.0 m–2). Overall, this research expands materials for osmotic energy–harvesting systems based on design decoupling paths biomass materials.

Language: Английский

Citations

13
Suppression of epileptic seizures by transcranial activation of K+-selective channelrhodopsin DOI Creative Commons
Xiao Duan, Chong Zhang,

Y. Wu

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 10, 2025

Optogenetics is a valuable tool for studying the mechanisms of neurological diseases and now being developed therapeutic applications. In rodents macaques, improved channelrhodopsins have been applied to achieve transcranial optogenetic stimulation. While photoexcitation neurons has achieved, noninvasive inhibition treating hyperexcitability-induced disorders remained elusive. There critical need effective inhibitory tools that are highly light-sensitive capable suppressing neuronal activity in deep brain tissue. this study, we sensitive moderately K+-selective channelrhodopsin (HcKCR1-hs) by molecular engineering recently discovered Hyphochytrium catenoides kalium (potassium) 1. Transcranial activation HcKCR1-hs significantly prolongs time first seizure, increases survival, decreases seizure several status epilepticus mouse models. Our approach neural hyperactivity may be adapted cell type-specific neuromodulation both basic preclinical settings. Optogenetic allowing seizures limited. This study demonstrates K + -conductive silences suppresses brain, alleviating epilepsy symptoms models, without intracranial surgery.

Language: Английский

Citations

1
The Cation−π Interaction in Chemistry and Biology DOI Creative Commons
Dennis A. Dougherty

Chemical Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 20, 2025

The cation-π interaction is an important noncovalent binding force that impacts all areas of chemistry and biology. Extensive computational gas phase experimental studies have established the potential strength essential nature interaction. Previous reviews emphasized model systems a variety biological examples. This work includes discussion those but emphasizes other are perhaps less well appreciated. These include novel ability alkali metals in water; application to organic synthesis chemical biology; cooperative behaviors multiple interactions, including adhesive proteins from mussels similar organisms formation modulation biomolecular condensates (phase separation); interactions involved recognizing DNA/RNA.

Language: Английский

Citations

1
Structures of channelrhodopsin paralogs in peptidiscs explain their contrasting K+ and Na+ selectivities DOI Creative Commons
Takefumi Morizumi, Kyumhyuk Kim, Hai Li

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: July 20, 2023

Kalium channelrhodopsin 1 from Hyphochytrium catenoides (HcKCR1) is a light-gated channel used for optogenetic silencing of mammalian neurons. It selects K

Language: Английский

Citations

15
Optogenetics and Related Technologies for Psychiatric Disease Research DOI
Lief E. Fenno, Karl Deisseroth

Oxford University Press eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 65 - 80

Published: Jan. 1, 2025

Abstract Causal study of brain function in model organisms is a central goal neuroscience, and this work critical to opening new frontiers the management psychiatric disease. Optogenetics—the use light-activated proteins directly control activity defined populations neurons awake, behaving animals—has revolutionized neuroscience being investigated for clinical direct treatment human neurological This chapter serves as an introduction development optogenetics, describes tool class modern systems applications understanding mental illness, outlines current efforts further develop technology. Readers can expect gain sufficient mechanistic optogenetics understand primary research, become familiar with molecular techniques, be able text reference reading.

Language: Английский

Citations

0
Optogenetic engineering for ion channel modulation DOI
Tianlu Wang, Teruo Nonomura,

Tien‐Hung Lan

et al.

Current Opinion in Chemical Biology, Journal Year: 2025, Volume and Issue: 85, P. 102569 - 102569

Published: Feb. 3, 2025

Language: Английский

Citations

0
Kalium channelrhodopsins effectively inhibit neurons DOI Creative Commons
Stanislav Ott, Sangyu Xu, Nicole Lee

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: April 24, 2024

The analysis of neural circuits has been revolutionized by optogenetic methods. Light-gated chloride-conducting anion channelrhodopsins (ACRs)-recently emerged as powerful neuron inhibitors. For cells or sub-neuronal compartments with high intracellular chloride concentrations, however, a conductance can have instead an activating effect. recently discovered light-gated, potassium-conducting, kalium (KCRs) might serve alternative in these situations, potentially broad application. As yet, KCRs not shown to confer potent inhibitory effects small genetically tractable animals. Here, we evaluated the utility suppress behavior and inhibit activity Drosophila, Caenorhabditis elegans, zebrafish. In direct comparisons ACR1, KCR1 variant enhanced plasma-membrane trafficking displayed comparable potency, but improved properties that include reduced toxicity superior efficacy putative high-chloride cells. This comparative behavioral inhibition between chloride- potassium-selective silencing tools establishes next-generation inhibitors for vivo circuit behaving

Language: Английский

Citations

4
Hydrated cation–π interactions of π-electrons with hydrated Mg2+ and Ca2+ cations DOI
Liuhua Mu, Guosheng Shi, Haiping Fang

et al.

The Journal of Chemical Physics, Journal Year: 2024, Volume and Issue: 160(21)

Published: June 6, 2024

Hydrated cation–π interactions at liquid–solid interfaces between hydrated cations and aromatic ring structures of carbon-based materials are pivotal in many material, biological, chemical processes, water serves as a crucial mediator these interactions. However, full understanding the alkaline earth structures, such graphene remains elusive. Here, we present molecular picture for Mg2+ Ca2+ by using density functional theory methods. Theoretical results show that sheet can distort hydration shell to interact with directly, which is water–cation–π In contrast, quite stable interacts indirectly, mediated molecules, cation–water–π These lead anomalous order adsorption energies cations, Mg2+–π < Ca2+–π when number molecules large (n ≥ 6), contrary observed absence = 0). The behavior adsorbed on surface mainly attributed competition effects. findings provide valuable details energy onto surface.

Language: Английский

Citations

4