Dissecting cardiovascular disease-associated noncoding genetic variants using human iPSC models

Stem Cell Reports, Journal Year: 2025, Volume and Issue: unknown, P. 102467 - 102467

Published: March 1, 2025

Language: Английский

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Cell Reprogramming, Transdifferentiation, and Dedifferentiation Approaches for Heart Repair

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3063 - 3063

Published: March 27, 2025

Cardiovascular disease (CVD) remains the leading cause of death globally, with myocardial infarction (MI) being a major contributor. The current therapeutic approaches are limited in effectively regenerating damaged cardiac tissue. Up-to-date strategies for heart regeneration/reconstitution aim at remodeling through repairing tissue an external cell source or by stimulating existing cells to proliferate and repopulate compromised area. Cell reprogramming is addressed this challenge as promising solution, converting fibroblasts other types into functional cardiomyocytes, either reverting pluripotent state directly switching lineage. Several such gene editing application miRNA small molecules have been explored their potential enhance regeneration. Those take advantage plasticity introducing factors that regress maturity vitro, allowing later differentiation thus endorsing transplantation, promote situ proliferation, leveraged scaffolds embedded pro-regenerative promoting efficient restoration. Despite notable advancements, important challenges persist, including low efficiency, maturation limitations, safety concerns clinical applications. Nonetheless, integrating these innovative offers alternative restoring function reducing dependency on full transplants.

Language: Английский

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HAND1 level controls the specification of multipotent cardiac and extraembryonic progenitors from human pluripotent stem cells

The EMBO Journal, Journal Year: 2025, Volume and Issue: unknown

Published: March 31, 2025

Abstract Diverse sets of progenitors contribute to the development embryonic heart, but mechanisms their specification have remained elusive. Here, using a human pluripotent stem cell (hPSC) model, we deciphered cardiac and non-cardiac lineage trajectories in differentiation identified transcription factors underpinning specification, identity function. We discovered concentration-dependent, fate determining function for basic helix-loop-helix factor HAND1 mesodermal uncovered its gene regulatory network. At low expression level, directs towards multipotent juxta-cardiac field able make cardiomyocytes epicardial cells, whereas at high level it promotes extraembryonic mesoderm. Importantly, HAND1-low can be propagated state. This detailed mechanistic insight into has potential accelerate delivery effective disease modelling, including congenital heart disease, therapy-based regenerative medicine.

Language: Английский

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Nanocomposite Hydrogels and Micro/Nanostructures for Printing Organoids

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: March 31, 2025

Organoids are 3D artificial miniature organs composed of a cluster self-renewing and self-organizing cells in vitro, which mimic the functions real organs. Nanotechnologies, including preparation nanomaterials fabrication micro/nanostructures, have been proven to promote cell proliferation, guide differentiation, regulate self-organization, showing great promise engineering organoids. In this Perspective, different types nanocomposite hydrogels for organoid culture summarized, effects micro/nanostructures on growth development discussed, bioprinting technologies constructing models introduced.

Language: Английский

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Molecular genetics of congenital heart disease

Science China Life Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

Language: Английский

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