Cell-type-specific striatal modulation of amygdalar acetylcholine in salience assignment DOI Creative Commons

Aixiao Chen,

Yunjing Li,

Hangfei Zhu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 31, 2024

ABSTRACT The salience assignment is pivotal for both natural and artificial intelligence. Pioneering studies established that basal forebrain cholinergic neurons process behaviorally relevant salient information. However, the neural circuit mechanism underlying remains poorly understood. Here we show acetylcholine (ACh) level in basolateral amygdala (BLA) dynamically represented behavioral salience. Distinct neuronal subpopulations nucleus accumbens (NAc), D1- D2-expressing medium spiny (MSNs), antagonistically specifically promote suppress ACh release BLA, but not cortex hippocampus. These striatal D1 D2 MSNs regulate BLA by disinhibiting inhibiting subregion substantia innominata (SI), respectively. Optogenetic manipulations of pathway from to SI opposingly affect associative learning. Our findings uncover an unconventional role via regulating salience-representing amygdalar activity.

Language: Английский

Prefrontal synaptic regulation of homeostatic sleep pressure revealed through synaptic chemogenetics DOI
Takeshi Sawada, Yusuke Iino, Kensuke Yoshida

et al.

Science, Journal Year: 2024, Volume and Issue: 385(6716), P. 1459 - 1465

Published: Sept. 26, 2024

Sleep is regulated by homeostatic processes, yet the biological basis of sleep pressure that accumulates during wakefulness, triggers sleep, and dissipates remains elusive. We explored a causal relationship between cellular synaptic strength electroencephalography delta power indicating macro-level developing theoretical framework molecular tool to manipulate strength. The mathematical model predicted increased promotes neuronal “down state” raises power. Our (synapse-targeted chemically induced translocation Kalirin-7, SYNCit-K), which induces dendritic spine enlargement potentiation through protein demonstrated excitatory neurons in prefrontal cortex (PFC) increases nonrapid eye movement amounts Thus, PFC dictates mammals.

Language: Английский

Citations

6
Layer 1 NDNF interneurons form distinct subpopulations with opposite activation patterns during sleep in freely behaving mice DOI Creative Commons
Aurélie Brécier,

Mailhos Gaëlle,

Przemysław Jarzębowski

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 11, 2025

ABSTRACT Non-rapid eye movement (NREM) sleep facilitates memory consolidation by transferring information from the hippocampus to neocortex. Recent evidence suggests that this transfer occurs primarily when hippocampal sharp-wave ripples (SWRs) and thalamocortical spindles are synchronized. In study, we asked what role cortical layer 1 NDNF-expressing (L1 NDNF) interneurons play during NREM in gating SWR-spindle synchronization. Using simultaneous cell-specific calcium imaging local field potential recordings freely moving mice, discovered L1 NDNF neurons form cell assemblies tuned specific stages, exhibiting differential responses spindle mediate slow inhibition through GABA B receptors. Systemic application of a receptor antagonist increased pyramidal neuron excitability sleep, enhanced inhibitory SWRs, disrupted coupling. Overall, these findings suggest an important contribution neuron-mediated synchronization oscillations with implications for processes.

Language: Английский

Citations

0
Basal Forebrain Projections to the Retrosplenial and Cingulate Cortex in Rats DOI Creative Commons
Hideki Kondo, László Záborszky

The Journal of Comparative Neurology, Journal Year: 2025, Volume and Issue: 533(2)

Published: Feb. 1, 2025

The basal forebrain (BF) plays a crucial role in modulating cortical activation through its widespread projections across the mantle. Previous anatomical studies have demonstrated that each region receives specific projection from BF. In this study, we examined BF cholinergic and non-cholinergic to retrosplenial cortex (RSC) anterior cingulate (ACC) using two retrograde tracers, Fast Blue (FB) Fluoro-Gold (FG), combination with choline acetyltransferase (ChAT) immunostaining rats. RSC ACC receive mainly medial part of horizontal limb diagonal band (HDB) vertical (VDB). main difference RSC, ACC, prelimbic (PL) is PL rostral half globus pallidus (GP), whereas stronger VDB septum (MS). As injection site shifts caudal strong GP weak MS/VDB neurons are reversed. Cholinergic make up similar proportion total both (37%) (33%) injections. Double tracer injections revealed small number double-labeled HDB. These findings indicate spatially overlapping differential BF, varying between subregions different rostrocaudal regions.

Language: Английский

Citations

0
Hierarchical organization of the forebrain cholinergic system in rats DOI Creative Commons

Peter Varsanyi,

Kevin D. Alloway, Candice M. Chavez

et al.

iScience, Journal Year: 2025, Volume and Issue: 28(3), P. 112001 - 112001

Published: Feb. 12, 2025

The basal forebrain (BF) cholinergic system (BFCS) participates in functions that are global across the brain, such as sleep-wake cycles, but also capacities more behaviorally and anatomically specific, including sensory perception. However, how it orchestrates all diverse local remains to be understood. To uncover underlying organization principles, we combined data from rat brains by tracing projections BF cortical areas analyzed spatial-numerical relations of neurons their targets. dataset revealed algorithmically identified hierarchically organized three principal networks: somatosensory-motor, auditory, visual, defined modality most predominant within them. These clusters could enable BFCS coordinate spatially selective signaling, parallel modulation multiple functionally interconnected yet groups areas. This previously unseen blueprint hierarchy is ready for functional testing.

Language: Английский

Citations

0
Dynamic changes in cortical neurotrophic factor-positive interneurons during sleep DOI Creative Commons
Christine Muheim,

Marcos G. Frank

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 21, 2025

Language: Английский

Citations

0
Oxytocin modulates inhibitory balance in the prelimbic cortex to support social memory consolidation during REM sleep DOI Creative Commons
Yanchao Liu,

Youyi Deng,

Zitao Zhu

et al.

Theranostics, Journal Year: 2025, Volume and Issue: 15(8), P. 3257 - 3274

Published: Feb. 18, 2025

Rationale: The prelimbic cortex (PrL), enriched with oxytocin (OXT) receptors, plays a critical role in memory consolidation. However, the of OXT social consolidation within PrL microcircuit remains poorly understood. Methods: To examine signaling consolidation, we used biosensors and loss-of-function approaches, including tetanus toxin-mediated silencing neurons paraventricular nucleus (PVNOXT), optogenetic inhibition PVNOXT-PrL pathway during rapid-eye-movement (REM) sleep, local administration an receptor antagonist PrL. In vivo molecular for vasoactive intestinal peptide (VIP), somatostatin, presynaptic calcium imaging were employed to assess inhibitory microcircuit. Optogenetic activation intranasal evaluate resilience chronic sleep deprivation-induced deficits. Results: We identified that REM-sleep release via PVN supports deficiency reduces activity VIP parvalbumin (PV) neurons, thereby disrupting balance between somatic mediated by PV dendritic disinhibition microcircuits REM sleep. Chronic deprivation (SD) disrupts balance, leading pyramidal neuron hyperactivity impairments. Notably, REM-sleep-specific or restores rescues deficits SD mice. Conclusion: Our results reveal how modulates support suggesting potential therapeutic strategies treating sleep-related disorders.

Language: Английский

Citations

0
Functional architecture of the forebrain cholinergic system in rodents DOI Creative Commons
László Záborszky,

Peter Varsanyi,

Kevin D. Alloway

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: June 19, 2024

The basal forebrain cholinergic system (BFCS) participates in functions that are global across the brain, such as sleep-wake cycles, but also capacities more behaviorally and anatomically specific, including sensory perception. To better understand underlying organization principles of BFCS, higher quality anatomical data analysis is needed. Here, we created a "virtual Basal Forebrain", combining from numerous rats with cortical retrograde tracer injections into common 3D reference coordinate space developed "spatial density correlation" methodology to analyze patterns BFCS projection targets, revealing organized three principal networks: somatosensory-motor, auditory, visual. Within each network, clusters cells increasing complexity innervate targets. These networks represent hierarchically building blocks may enable spatially selective signaling, parallel modulation multiple functionally interconnected yet diverse groups areas.

Language: Английский

Citations

1
Attention-Based CNN-BiLSTM for Sleep State Classification of Spatiotemporal Wide-Field Calcium Imaging Data DOI Creative Commons
Xiaohui Zhang, Eric C. Landsness, Hanyang Miao

et al.

Journal of Neuroscience Methods, Journal Year: 2024, Volume and Issue: 411, P. 110250 - 110250

Published: Aug. 14, 2024

Wide-field calcium imaging (WFCI) with genetically encoded indicators allows for spatiotemporal recordings of neuronal activity in mice. When applied to the study sleep, WFCI data are manually scored into sleep states wakefulness, non-REM (NREM) and REM by use adjunct EEG EMG recordings. However, this process is time-consuming, invasive often suffers from low inter- intra-rater reliability. Therefore, an automated state classification method that operates on desired.

Language: Английский

Citations

1
Optogenetic Brain–Computer Interfaces DOI Creative Commons
Feifang Tang, Feiyang Yan,

Yushan Zhong

et al.

Bioengineering, Journal Year: 2024, Volume and Issue: 11(8), P. 821 - 821

Published: Aug. 12, 2024

The brain-computer interface (BCI) is one of the most powerful tools in neuroscience and generally includes a recording system, processor stimulation system. Optogenetics has advantages bidirectional regulation, high spatiotemporal resolution, cell-specific which expands application scenarios BCIs. In recent years, optogenetic BCIs have become widely used lab with development materials software. systems were designed to be more integrated, lightweight, biocompatible, power efficient, as wireless transmission chip-level embedded software also constantly improving, better real-time performance accuracy lower consumption. On other hand, cutting-edge technology spanning multidisciplinary fields including molecular biology, neuroscience, material engineering, information processing, great potential neural decoding, enhancing brain function, treating diseases. Here, we review future, combined functional imaging techniques such near-infrared spectroscopy (fNIRS) magnetic resonance (fMRI), can modulate function specific circuits, facilitate neurological rehabilitation, assist perception, establish brain-to-brain interface, applied wider scenarios.

Language: Английский

Citations

1
Cell-type-specific striatal modulation of amygdalar acetylcholine in salience assignment DOI Creative Commons

Aixiao Chen,

Yunjing Li,

Hangfei Zhu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 31, 2024

ABSTRACT The salience assignment is pivotal for both natural and artificial intelligence. Pioneering studies established that basal forebrain cholinergic neurons process behaviorally relevant salient information. However, the neural circuit mechanism underlying remains poorly understood. Here we show acetylcholine (ACh) level in basolateral amygdala (BLA) dynamically represented behavioral salience. Distinct neuronal subpopulations nucleus accumbens (NAc), D1- D2-expressing medium spiny (MSNs), antagonistically specifically promote suppress ACh release BLA, but not cortex hippocampus. These striatal D1 D2 MSNs regulate BLA by disinhibiting inhibiting subregion substantia innominata (SI), respectively. Optogenetic manipulations of pathway from to SI opposingly affect associative learning. Our findings uncover an unconventional role via regulating salience-representing amygdalar activity.

Language: Английский

Citations

0