Spatial model of cell-fate choice uncovers strong links between tissue morphology and tissue regeneration DOI Creative Commons
Somya Mani, Tsvi Tlusty

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 26, 2024

Tissues in multicellular organisms are immensely diverse: animal tissues include sheet-like epithelia, bundles of syncitial muscle fibres and the branched interconnected nerves, while plants contain epidermis highly organized vascular tissue. However, at microanatomical level, notably similar that they into distinct domains: domains characterized by their cellular compositions hold precise adjacency relations among each other. These morphological similarities surprising because multicellularity has evolved multiple times independently. Separately, also a remarkable functional similarity: across all organisms, including poor regenerators such as mammals, routinely heal from injuries. The organization within tissues, well ability regenerate result developmental processes: cells divide, die, differentiate migrate according to cues receive neighborhoods. We ask two interlinked questions: What diversity tissue morphology can simple processes generate? And is related regeneration? address these questions using an agent based model cell-fate decisions where use rules respond neighborhoods Our produces rich morphologies: By simply tuning density interactions propensity differentiation, we produce go disordered sparse dense contiguous domains. Importantly, was strongly linked regeneration model: enriched densely packed, tissues. Moreover, predominant mode healing recapitulates natural mechanisms: healed through replacement injured cell-division adjacent regions. work generates experimentally testable predictions on effects manipulating turn, regeneration.

Language: Английский

Optimal control of spatial diseases spreading in networked reaction–diffusion systems DOI
Gui‐Quan Sun,

Runzi He,

Li-Feng Hou

et al.

Physics Reports, Journal Year: 2025, Volume and Issue: 1111, P. 1 - 64

Published: Feb. 1, 2025

Language: Английский

Citations

0
Dual PAX3/7 transcriptional activities spatially encode spinal cell fates through distinct gene networks DOI Creative Commons
Robin Rondon,

Théaud Hezez,

Julien Richard Albert

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 12, 2025

Abstract Understanding how transcription factors regulate organized cellular diversity in developing tissues remains a major challenge due to their pleiotropic functions. We addressed this by monitoring and genetically modulating the activity of PAX3 PAX7 during specification neural progenitor pools embryonic spinal cord. Using mouse models, we show that balance between transcriptional activating repressing functions these is modulated along dorsoventral axis instructive patterning pools. By combining loss-of-function experiments with functional genomics organoids, demonstrate PAX-mediated repression activation rely on distinct cis- regulatory genomic modules. This enables both coexistence dual dorsal cell progenitors specific control two differentiation programs. PAX promotes H3K27me3 deposition at silencers repress ventral identities, while enhancers, they act as pioneer factors, opening modules specify dorsal-most identities. Finally, restricted cells exposed BMP morphogens, ensuring spatial specificity. These findings reveal proteins, morphogen gradients, orchestrate neuronal cord, providing robust framework for subtype specification.

Language: Английский

Citations

0
Organizational principles of integumentary Organs: Maximizing Variations for Effective Adaptation DOI
Cheng‐Ming Chuong, Ping Wu, Yu Zhou

et al.

Developmental Biology, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

0
À l’origine du vivant, la différence sans hiérarchie(s) DOI Creative Commons
Jean‐Christophe Pagès

médecine/sciences, Journal Year: 2025, Volume and Issue: 41(3), P. 282 - 285

Published: March 1, 2025

Citations

0
Synchronization of the segmentation clock using synthetic cell-cell signaling DOI Creative Commons
Akihiro Isomura,

Daisuke Asanuma,

Ryoichiro Kageyama

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 4, 2024

Tight coordination of cell-cell signaling in space and time is vital for self-organization tissue patterning. During vertebrate development, the segmentation clock drives oscillatory gene expression presomitic mesoderm (PSM), leading to periodic formation somites. Oscillatory synchronized at cell population level; inhibition Delta-Notch results loss synchrony fusion However, it remains unclear how couples controls synchronization. Here, we report reconstitution oscillation PSM organoids by synthetic with designed ligand-receptor pairs. Optogenetic assays uncovered that intracellular domains ligands play key roles dynamic communication. coupling using recovered cells deficient signaling; non-oscillatory did not induce recovery. This study reveals mechanism which molecules coordinate synchronization clock, provides direct evidence communication clock.

Language: Английский

Citations

2
Metabolic adaptation drives self-organization during skin organoid morphogenesis DOI Creative Commons
Mingxing Lei, Jingwei Jiang, Weiwei Liu

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 23, 2024

Abstract Self-organization in organoid morphogenesis involves the coordinated arrangement of interacting cells into higher-order structures, yet underlying principles remain elusive. Here, we investigate how epidermal and dermal respond distinctively to elevated levels hypoxia during skin that largely resembles development embryogenesis. We unveil autonomously generated hypoxic environment-induced metabolic adaptation drives transition from coalesced spheroids a planarized structure organoids through following three levels. Hif1a-mediated anaerobic metabolism positions liquid phase cultures under lower oxygen level, facilitating tissue separation layer layer. Hypoxia-driven activation lysosomal hydrolases eliminates suprabasal keratin debris planar epidermis formation. Fibroblasts adjacent basal have differential hypoxia, which exhibit enhanced retinoid become putative papillary dermis. Together, these hypoxia-induced adaptations contribute reconstructing architecture similar physiological development. Our findings highlight ability alteration trigger varied cellular responses, leading self-organizing spheroids-to-planar topological transformations restoration homeostasis.

Language: Английский

Citations

0
Spatial model of cell-fate choice uncovers strong links between tissue morphology and tissue regeneration DOI Creative Commons
Somya Mani, Tsvi Tlusty

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 26, 2024

Tissues in multicellular organisms are immensely diverse: animal tissues include sheet-like epithelia, bundles of syncitial muscle fibres and the branched interconnected nerves, while plants contain epidermis highly organized vascular tissue. However, at microanatomical level, notably similar that they into distinct domains: domains characterized by their cellular compositions hold precise adjacency relations among each other. These morphological similarities surprising because multicellularity has evolved multiple times independently. Separately, also a remarkable functional similarity: across all organisms, including poor regenerators such as mammals, routinely heal from injuries. The organization within tissues, well ability regenerate result developmental processes: cells divide, die, differentiate migrate according to cues receive neighborhoods. We ask two interlinked questions: What diversity tissue morphology can simple processes generate? And is related regeneration? address these questions using an agent based model cell-fate decisions where use rules respond neighborhoods Our produces rich morphologies: By simply tuning density interactions propensity differentiation, we produce go disordered sparse dense contiguous domains. Importantly, was strongly linked regeneration model: enriched densely packed, tissues. Moreover, predominant mode healing recapitulates natural mechanisms: healed through replacement injured cell-division adjacent regions. work generates experimentally testable predictions on effects manipulating turn, regeneration.

Language: Английский

Citations

0