Spotlight on “A protein blueprint of the diatom CO 2 -fixing organelle” by Nam et al. (2024) and “Diatom pyrenoids are encased in a protein shell that enables efficient CO 2 fixation” by Shimakawa et al. (2024) DOI Creative Commons

Weiyang Chen,

Dario Leister

Journal of Mitochondria Plastids and Endosymbiosis, Journal Year: 2024, Volume and Issue: 3(1)

Published: Dec. 24, 2024

Recent studies by Nam et al. and Shimakawa published in Cell (2024) have elucidated the protein composition of diatom pyrenoids constructing protein–protein interaction networks for centric Thalassiosira pseudonana using vivo photo-crosslinking with Rubisco pennate Phaeodactylum tricornutum. These investigations identified a family proteins that form pyrenoid sheath, termed "Shell" proteins. Knockout experiments revealed absence or malformation these sheaths leads to altered structures impaired growth under atmospheric CO2 conditions. The research highlights are enclosed lattice-like proteinaceous sheaths, as demonstrated through multiscale imaging techniques. findings provide insights into convergent evolution across various algal lineages suggest potential strategies engineering crop plants enhance agricultural yields.

Language: Английский

A protein blueprint of the diatom CO2-fixing organelle DOI Creative Commons
Onyou Nam,

Sabina Musiał,

Manon Demulder

et al.

Cell, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 1, 2024

Language: Английский

Citations

8
DiatOmicBase: a versatile gene‐centered platform for mining functional omics data in diatom research DOI Creative Commons
Émilie Villar, Nathanaël Zweig, Pierre Vincens

et al.

The Plant Journal, Journal Year: 2025, Volume and Issue: 121(6)

Published: March 1, 2025

SUMMARY Diatoms are prominent microalgae found in all aquatic environments. Over the last 20 years, thanks to availability of genomic and genetic resources, diatom species such as Phaeodactylum tricornutum Thalassiosira pseudonana have emerged valuable experimental model systems for exploring topics ranging from evolution cell biology, (eco)physiology, biotechnology. Since first genome sequencing projects initiated more than years ago, numerous genome‐enabled datasets been generated, based on RNA‐Seq proteomics experiments, epigenomes, ecotype variant analysis. Unfortunately, these generated by various laboratories, often disparate formats challenging access analyze. Here we present DiatOmicBase, a portal gathering comprehensive omics resources P. T. facilitate exploration dispersed public design new experiments prior‐art. DiatOmicBase provides gene annotations, transcriptomic profiles browser with variants, histone methylation marks, transposable elements, non‐coding RNAs, read densities experiments. We developed semi‐automatically updated module explore both publicly available users' private datasets. Using gene‐level expression data, users can perform exploratory data analysis, differential expression, pathway biclustering, co‐expression network Users create heatmaps visualize pre‐computed comparisons selected subsets. Automatic other bioinformatic tools comparative functional genomics is also provided. Focusing currently centralized , showcase several examples how strengthens molecular research diatoms, making organisms accessible broad community.

Language: Английский

Citations

0
Overexpression of Plastid Acetyl-CoA Carboxylase Confers Stress Tolerances with Increased Levels of Unsaturated Fatty Acids in the Marine Diatom Phaeodactylum tricornutum DOI
Masakazu Toyoshima, H. Okuda,

Yuya Okada

et al.

Marine Biotechnology, Journal Year: 2025, Volume and Issue: 27(2)

Published: March 26, 2025

Language: Английский

Citations

0
Multiphase CO2-dependent photosynthesis in marine diatoms DOI Creative Commons
Ginga Shimakawa, Yusuke Matsuda

Frontiers in Photobiology, Journal Year: 2025, Volume and Issue: 3

Published: April 2, 2025

Diatoms, one of the most ubiquitous phytoplankton in oceans, have evolved a pyrenoid-based CO 2 -concentrating mechanism (CCM) to utilize limited seawater for photosynthesis. Recent proteomics analyses and molecular biological tools deepened our understanding mechanisms involved diatom chloroplast architecture CCM. Here, we provide an update knowledge processes high affinity photosynthesis dissolved inorganic carbon (DIC) diatoms. Based on phenotype genome-edited mutants, propose model CCM composed four phases -dependent at (I) less than 0.1 mM, (II) 0.1–2 (III) 2–10 (IV) more 10 mM DIC concentrations, which rate-determining steps are capture unfixed stroma Phases I II, evolution pyrenoid-penetrating thylakoid lumen Phase III, transport IV. Under natural containing mainly form HCO 3 − , marine diatoms is likely primarily shifting II when available limited.

Language: Английский

Citations

0
New avenues in photosynthesis: from light harvesting to global modeling DOI
Tiina Tosens, Alessandro Alboresi, Herbert van Amerongen

et al.

Physiologia Plantarum, Journal Year: 2025, Volume and Issue: 177(2)

Published: March 1, 2025

Abstract Photosynthesis underpins life on Earth, serving as the primary energy source while regulating global carbon and water cycles, thereby shaping climate vegetation. Advancing photosynthesis research is essential for improving crop productivity refining models across scales, ultimately addressing critical challenges such food security environmental sustainability. This minireview synthesizes a selection of recent advancements presented at 2nd European Congress Research, focusing efficiency modelling scales. We explore strategies to optimize light harvesting fixation, leading canopy level improvements. Alongside synthetic biology, we examine advances in harnessing natural variability key photosynthetic traits, considering both methodological innovations vast reservoir opportunities they present. Additionally, highlight unique insights gained from plants adapted extreme environments, offering pathways improve resilience simultaneously. emphasize importance holistic approach, integrating dynamic modeling metabolic processes bridge these advancements. Beyond improvements, discuss progress simulations, particularly through improved parametrization mesophyll conductance, crucial enhancing leaf‐to‐global scale simulations. Recognizing need greater interdisciplinary collaboration tackle grand put research, two initiatives launched congress—an open science platform dedicated journal plant ecophysiology. conclude this with forward‐looking outline, highlighting next steps toward achieving meaningful improvements photosynthesis, yield, modeling.

Language: Английский

Citations

0
From algae to plants: understanding pyrenoid-based CO2-concentrating mechanisms DOI Creative Commons
Ella Catherall, Sabina Musiał, Nicky Atkinson

et al.

Trends in Biochemical Sciences, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Language: Английский

Citations

0
Spotlight on “A protein blueprint of the diatom CO 2 -fixing organelle” by Nam et al. (2024) and “Diatom pyrenoids are encased in a protein shell that enables efficient CO 2 fixation” by Shimakawa et al. (2024) DOI Creative Commons

Weiyang Chen,

Dario Leister

Journal of Mitochondria Plastids and Endosymbiosis, Journal Year: 2024, Volume and Issue: 3(1)

Published: Dec. 24, 2024

Recent studies by Nam et al. and Shimakawa published in Cell (2024) have elucidated the protein composition of diatom pyrenoids constructing protein–protein interaction networks for centric Thalassiosira pseudonana using vivo photo-crosslinking with Rubisco pennate Phaeodactylum tricornutum. These investigations identified a family proteins that form pyrenoid sheath, termed "Shell" proteins. Knockout experiments revealed absence or malformation these sheaths leads to altered structures impaired growth under atmospheric CO2 conditions. The research highlights are enclosed lattice-like proteinaceous sheaths, as demonstrated through multiscale imaging techniques. findings provide insights into convergent evolution across various algal lineages suggest potential strategies engineering crop plants enhance agricultural yields.

Language: Английский

Citations

0