Chemical reprogramming culture for the expansion of salivary gland epithelial basal progenitor cells

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 18, 2025

Abstract Background Salivary gland (SG) hypofunction presents a significant clinical challenge with limited treatment options. SG epithelial cells offer promising approach due to their intrinsic tissue specificity and regenerative potential. However, the lack of efficient culture methods has hindered use. Methods This study chemical reprogramming (CRC) system that utilizes combination three small molecules for long-term two-dimensional human progenitor cells. We characterized cultured cells, measured organoid-forming efficiencies, assessed differentiation To evaluate therapeutic efficacy basal (SG-BPCs), we administered them into mouse model radiation-induced functional recovery. Results By utilizing optimal concentrations Y-27632, A83-01, LDN193189, achieved over 50 population doubling levels (PD) within 80 d, surpassing Hayflick limit. β-galactosidase Terminal deoxynucleotidyl transferase dUTP nick end labeling staining confirmed these inhibited cellular senescence apoptosis, respectively. The expressed ductal cell markers KRT5, KRT19, SOX9, increased expression observed from PD5 PD40. Notably, expanded were able differentiate various types, including acinar myoepithelial indicating SG-basal (SG-BPCs) selectively proliferated using our CRC method. assess potential SG-BPCs, they mice hypofunction. successfully restored function. Conclusion Our findings demonstrate is an effective method SG-BPCs. advancement holds promise development progenitor-based therapies treat

Language: Английский

Urine PD-L1 as a Non-Invasive Biomarker for Immune Checkpoint Inhibitor (ICI) Therapy in Bladder Cancer

Advances in Biomarker Sciences and Technology, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

Unveiling the role of nanoparticle-based therapeutic strategies for pulmonary drug delivery

Journal of Drug Delivery Science and Technology, Journal Year: 2024, Volume and Issue: unknown, P. 106558 - 106558

Published: Dec. 1, 2024

Language: Английский