Glutamate Supplementation Regulates Nitrogen Metabolism in the Colon and Liver of Weaned Rats Fed a Low-Protein Diet DOI Open Access
Da Jiang, Jing Zhang, Yun Ji

et al.

Nutrients, Journal Year: 2025, Volume and Issue: 17(9), P. 1465 - 1465

Published: April 26, 2025

Background: Glutamate, a nutritionally non-essential amino acid, is key intermediate in nitrogen metabolism. Despite more studies on its functional role intestine health, it remains unknown how glutamate regulates metabolism animals fed low-protein diet. Methods: Herein, we investigated the effects of supplementation colonic acid transport, barrier protein expression, microbiota alterations, fecal emissions, hepatic and synthesis weaned rats. Results: We found that restriction diminished mucus thickness, reduced goblet cell numbers, expression EAAT3, y+LAT2 colon. In contrast, reversed these effects, increasing colon length enhancing ZO-1, Occludin, Claudin-1 At genus level, increased abundance Lactococcus Clostridia_sensu_stricto_18. Additionally, resulted an apparent digestibility, ratio to total intake, microbial intake. Protein decreased mRNA level ATP1A1, SNAT9/2, ASCT2, p-mTOR, mTOR, p-mTOR/mTOR, p-p70S6K/p70S6K as well p-4EBP1/4EBP1 liver. These were by supplementation. Conclusions: conclusion, upregulates transporters colon, modulates composition reduce excretion, enhances transport liver activating mTOR/p70S6K/4EBP1 pathway, which influences rats

Language: Английский

Glutamate Supplementation Regulates Nitrogen Metabolism in the Colon and Liver of Weaned Rats Fed a Low-Protein Diet DOI Open Access
Da Jiang, Jing Zhang, Yun Ji

et al.

Nutrients, Journal Year: 2025, Volume and Issue: 17(9), P. 1465 - 1465

Published: April 26, 2025

Background: Glutamate, a nutritionally non-essential amino acid, is key intermediate in nitrogen metabolism. Despite more studies on its functional role intestine health, it remains unknown how glutamate regulates metabolism animals fed low-protein diet. Methods: Herein, we investigated the effects of supplementation colonic acid transport, barrier protein expression, microbiota alterations, fecal emissions, hepatic and synthesis weaned rats. Results: We found that restriction diminished mucus thickness, reduced goblet cell numbers, expression EAAT3, y+LAT2 colon. In contrast, reversed these effects, increasing colon length enhancing ZO-1, Occludin, Claudin-1 At genus level, increased abundance Lactococcus Clostridia_sensu_stricto_18. Additionally, resulted an apparent digestibility, ratio to total intake, microbial intake. Protein decreased mRNA level ATP1A1, SNAT9/2, ASCT2, p-mTOR, mTOR, p-mTOR/mTOR, p-p70S6K/p70S6K as well p-4EBP1/4EBP1 liver. These were by supplementation. Conclusions: conclusion, upregulates transporters colon, modulates composition reduce excretion, enhances transport liver activating mTOR/p70S6K/4EBP1 pathway, which influences rats

Language: Английский

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