CDCA genes as prognostic and therapeutic targets in Colon adenocarcinoma

Hereditas, Journal Year: 2025, Volume and Issue: 162(1)

Published: Feb. 10, 2025

Abstract Objectives The study investigates the role of Cell Division Cycle Associated (CDCA) genes in colorectal cancer (COAD) by analyzing their differential expression, epigenetic alterations, prognostic significance, and functional associations. Methodology This employed a detailed silico vitro experiments-based methodology. Results RT-qPCR assays reveal significantly elevated mRNA levels CDCA2, CDCA3, CDCA4, CDCA5, CDCA7, CDCA8 COAD cell lines compared to controls. Bisulfite sequencing indicates reduced promoter methylation CDCA gene promoters lines, suggesting an regulatory mechanism. Analysis large TCGA datasets confirms increased expression tissues. Survival analysis using cSurvival database demonstrates negative correlations between patient overall survival. Additionally, Lasso regression-based models predict survival outcomes patients. Investigating immune modulation, inversely correlates with infiltration modulators. miRNA-mRNA network identifies miRNAs targeting genes, validated showing up-regulation has-mir-10a-5p has-mir-20a-5p Drug sensitivity suggests resistance specific drugs patients expression. Furthermore, crucial states COAD, including “angiogenesis, apoptosis, differentiation, hypoxia, inflammation, metastasis.” Additional experiments revealed that CDCA2 CDCA3 knockdown SW480 SW629 cells proliferation colony formation while enhancing migration. Conclusion Overall, elucidates multifaceted progression, providing insights into potential diagnostic, prognostic, therapeutic implications.

Language: Английский

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Advances of Artificial Intelligence in Clinical Application and Scientific Research of Neuro-oncology: Current Knowledge and Future Perspectives

Critical Reviews in Oncology/Hematology, Journal Year: 2025, Volume and Issue: unknown, P. 104682 - 104682

Published: March 1, 2025

Brain tumors refer to the abnormal growths that occur within brain's tissue, comprising both primary neoplasms and metastatic lesions. Timely detection, precise staging, suitable treatment, standardized management are of significant clinical importance for extending survival rates brain tumor patients. Artificial intelligence (AI), a discipline computer science, is leveraging its robust capacity information identification combination revolutionize traditional paradigms oncology care, offering substantial potential precision medicine. This article provides an overview current applications AI in tumors, encompassing technologies, their working mechanisms workflow, contributions diagnosis as well role scientific research, particularly drug innovation revealing microenvironment. Finally, paper addresses existing challenges, solutions, future application prospects. review aims enhance our understanding provide valuable insights forthcoming inquiries.

Language: Английский

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Vitamin K2 mitigates cognitive, and motor impairments induced by multiple surgery with anesthesia and analgesia in neonatal stage

Biochemical and Biophysical Research Communications, Journal Year: 2025, Volume and Issue: unknown, P. 151784 - 151784

Published: April 1, 2025

Anesthesia and analgesia are essential components for surgical procedures. While the neurotoxic effects of multiple anesthesia exposures during brain development well established, combined impact surgery with on neurodevelopmental remains unknown. In this study, neonatal mice underwent fentanyl sevoflurane (MSFS) postnatal days 6, 8, 10, resulting in attention deficit hyperactivity disorder (ADHD)-like hyperactivity, impulsive behavior, cognitive impairment, fine motor dysfunction adulthood. Additionally, MSFS inhibited neurogenesis nucleus accumbens (NAc) by reducing neural stem cells (NSCs) proliferation differentiation into neurons astrocytes. Pre-administration Vitamin K2 (VK2) 30 min before each procedure significantly mitigated behavioral impairments restored neurogenesis. RNA-sequencing revealed that treatment induced 75 up-regulated 140 down-regulated differentially expressed genes (DEGs) NAc, while VK2 pre-administration resulted 149 56 DEGs. Among these, 32 DEGs were but 12 VK2. To identify key regulatory modulated VK2, we performed protein-protein interaction analysis using CytoHubba, which 10 hub genes-DLGAP5, TPX2, KIF20B, PLK1, SGO1, GTSE1, ASPM, CDCA2, BUB1B, NUSAP1-with critical roles cell cycle, division NSCs pathways. The expression was validated RT-qPCR immunofluorescence staining. These findings suggest MSFS-induces ADHD-like behaviors, dysfunction, impaired altering involved pathways, rescued This study presents clinically model investigating toxicity highlights VK2's potential as a neuroprotective agent pediatric involving analgesia.

Language: Английский

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The Role of CDCA2 in Tumor Genesis, Prognosis and Future Treatments

European Journal of Cancer, Journal Year: 2024, Volume and Issue: 211, P. 114308 - 114308

Published: Sept. 6, 2024

Language: Английский

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Machine learning in oncological pharmacogenomics: advancing personalized chemotherapy

Functional & Integrative Genomics, Journal Year: 2024, Volume and Issue: 24(5)

Published: Oct. 1, 2024

Language: Английский

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