AEBP1 Silencing Protects Against Cerebral Ischemia/Reperfusion Injury by Regulating Neuron Ferroptosis and Microglia M2 Polarization Through PRKCA‐PI3K‐Akt Axis DOI
Yafen Zhang, Yan Li,

Fengli Liu

et al.

Drug Development Research, Journal Year: 2024, Volume and Issue: 85(8)

Published: Dec. 1, 2024

ABSTRACT Cerebral ischemia/reperfusion injury is one of the main causes neuronal damage. Neuron ferroptosis and microglia polarization are considered as critical processes during cerebral ischemia/reperfusion. Adipocyte enhancer‐binding protein 1 (AEBP1) usually acts a transcriptional repressor which involved in various diseases. However, it still remains unknown whether AEBP1 could have important roles regulating neuron injury. The oxygen‐glucose deprivation reperfusion (OGD/R)‐treated cells middle artery occlusion (MCAO)‐treated mice were used vitro vivo models. differentially expressed factors analyzed according to GEO datasets. Relative mRNA expression levels detected by qRT‐PCR western blot analysis. Cell viability was measured CCK‐8 assay. ROS, GSH iron contents using specifical assay kits. CD26 CD206 immunofluorescence Inflammatory cytokines ELISA. association between PRKCA assessed luciferase reporter ChIP analyses. damage TTC staining neurological deficit score. Transcription factor increased OGD/R‐treated HT22 BV2 cells. silencing attenuated OGD/R‐induced cell through increasing viability, GPX4 levels, decreasing ACSL4 levels. knockdown promoted M2 CD206‐positive Arg‐1 level, reducing iNOS, TNF‐α, IL‐1β IL‐6 transcriptionally repressed expression, further regulated PI3K/Akt signaling activation. Inhibition or reversed effects on polarization. downregulation infarct size scores MCAO‐treated mice. mitigated activating signaling, indicating potentially protective action

Language: Английский

Assessing the 53‐Year Epidemiological Trends of Oral Squamous Cell Carcinoma (OSCC) in Northeastern Iran DOI Creative Commons
Nasrollah Saghravanian,

Yasamin Tajdini,

Pooya Saeedi

et al.

Clinical and Experimental Dental Research, Journal Year: 2025, Volume and Issue: 11(1)

Published: Feb. 1, 2025

ABSTRACT Objectives This study aimed to assess the epidemiological trends of oral squamous cell carcinoma (OSCC) in Northeast Iran over a 53‐year period. Material and Methods In this retrospective study, we obtained data on OSCC cases spanning 53 years (1970–2022), including demographic details, such as age, sex, site, histopathological grade. Descriptive analysis was conducted, frequencies were compared using chi‐squared test evaluate differences between sexes, age groups, cancer sites, grades. Additionally, assessed associations each site with Statistical significance set at p < 0.05, confidence interval 95%. Results examined 13,769 cases, among which 507 diagnosed OSCC, accounting for 3.68% total cases. The mean 59.27 ± 14.94 years, significantly higher prevalence individuals older than 50 ( 0.001). There nearly equal proportions males females, tongue being most common (28.4%), followed by gingiva (26.4%) buccal mucosa (19.5%). Grade 1 (well differentiated) more prevalent (54%) other significant association sex SCC females = 0.006) lip 0.008). Prevalence above tongue, mucosa, gingiva, 0.001, 0.028, respectively). floor mouth, grade Conclusions Our revealed that predominantly affects sexes. commonly affected sites are often exhibiting findings. Enhanced community awareness risk factors regular examinations essential reducing incidence OSCC.

Language: Английский

Citations

0
Inflammatory Biomarkers and Oral Health Disorders as Predictors of Head and Neck Cancer: A Retrospective Longitudinal Study DOI Open Access
Amr Sayed Ghanem, Kitti Sipos, Ágnes Tóth

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 2279 - 2279

Published: March 4, 2025

Head and neck cancers (HNCs) are often diagnosed late, leading to poor prognosis. Chronic inflammation, particularly periodontitis, has been linked carcinogenesis, but systemic inflammatory markers remain underexplored. This study was the first examine whether elevated C-reactive protein (CRP) can serve as a cost-effective adjunct in HNC risk assessment, alongside oral health indicators. A retrospective cohort analysed 23,742 hospital records (4833 patients, 2015-2022) from University Hospital of Debrecen. cases were identified using ICD-10 codes, with CRP periodontitis key predictors. Kaplan-Meier survival analysis, log-rank tests, Weibull regression used assess risk, model performance evaluated via AIC/BIC ROC curves. Periodontitis significantly associated (HR 5.99 [1.96-18.30]), while (>15 mg/L) independently increased 4.16 [1.45-12.00]). Females had lower than males 0.06 [0.01-0.50]). may cost-effective, easily accessible biomarker for early detection when combined screening. Integrating inflammation into assessment models could potentially improve diagnosis high-risk populations.

Language: Английский

Citations

0
A bibliometric analysis of programmed cell death in oral cancer literature: research patterns and emerging trends (2000–2024) DOI Creative Commons

Wenli Gu

Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 22, 2025

Language: Английский

Citations

0
AEBP1 Silencing Protects Against Cerebral Ischemia/Reperfusion Injury by Regulating Neuron Ferroptosis and Microglia M2 Polarization Through PRKCA‐PI3K‐Akt Axis DOI
Yafen Zhang, Yan Li,

Fengli Liu

et al.

Drug Development Research, Journal Year: 2024, Volume and Issue: 85(8)

Published: Dec. 1, 2024

ABSTRACT Cerebral ischemia/reperfusion injury is one of the main causes neuronal damage. Neuron ferroptosis and microglia polarization are considered as critical processes during cerebral ischemia/reperfusion. Adipocyte enhancer‐binding protein 1 (AEBP1) usually acts a transcriptional repressor which involved in various diseases. However, it still remains unknown whether AEBP1 could have important roles regulating neuron injury. The oxygen‐glucose deprivation reperfusion (OGD/R)‐treated cells middle artery occlusion (MCAO)‐treated mice were used vitro vivo models. differentially expressed factors analyzed according to GEO datasets. Relative mRNA expression levels detected by qRT‐PCR western blot analysis. Cell viability was measured CCK‐8 assay. ROS, GSH iron contents using specifical assay kits. CD26 CD206 immunofluorescence Inflammatory cytokines ELISA. association between PRKCA assessed luciferase reporter ChIP analyses. damage TTC staining neurological deficit score. Transcription factor increased OGD/R‐treated HT22 BV2 cells. silencing attenuated OGD/R‐induced cell through increasing viability, GPX4 levels, decreasing ACSL4 levels. knockdown promoted M2 CD206‐positive Arg‐1 level, reducing iNOS, TNF‐α, IL‐1β IL‐6 transcriptionally repressed expression, further regulated PI3K/Akt signaling activation. Inhibition or reversed effects on polarization. downregulation infarct size scores MCAO‐treated mice. mitigated activating signaling, indicating potentially protective action

Language: Английский

Citations

0