Gene, Journal Year: 2025, Volume and Issue: 959, P. 149518 - 149518
Published: April 18, 2025
Language: Английский
Molecular Carcinogenesis, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 17, 2025
ABSTRACT METTL1, a well‐established RNA methyltransferase for the N(7)‐methylguanosine (m7G) methylation modification, is responsible human tumorigenesis. Here, we aimed to examine activity and molecular determinants of METTL1 in colorectal cancer (CRC) development. ribosomal processing 9 (RRP9) mRNA analysis was performed by quantitative PCR. Protein expression detected immunoblotting immunohistochemistry (IHC). Cell sphere formation, invasion, proliferation were assessed transwell, MTT assays, respectively. migration tested transwell wound healing assays. Subcutaneous xenografts produced analyze role vivo. The influence m7G stability RRP9 evaluated methylated immunoprecipitation (MeRIP) assay Actinomycin D (Act D) treatment, highly expressed CRC tumors cell lines. depletion suppressed proliferation, invasiveness, migratory ability, formation potential vitro, while increased had opposite effects. positively correlated with CRC. Mechanistically, promoted mediating its methylation, regulated PI3K/AKT signaling RRP9. Increased partially reversed suppressive effects on phenotypes vitro. impeded growth HCT‐116 subcutaneous vivo Our observations identified as crucial protumorigenic factor drive growth, metastasis, stemness cells through RRP9, offering new targets combating
Language: Английский
Citations
1
BioEssays, Journal Year: 2024, Volume and Issue: unknown
Published: Nov. 26, 2024
ABSTRACT Transfer RNA (tRNA) modifications play an important role in regulating mRNA translation at the codon level. tRNA can influence selection and optimality, thus shifting toward specific sets of mRNAs a dynamic manner. Queuosine (Q) is modification occurring wobble position. In eukaryotes, queuosine synthesized by tRNA‐guanine trans ‐glycosylase (TGT) complex, which incorporates nucleobase queuine (or Qbase) into guanine GUN anticodons. Queuine sourced from gut bacteria dietary intake. Q was recently shown to be critical for cellular responses oxidative mitochondrial stresses, as well its potential neurodegenerative diseases brain health. These unique features provide interesting insight regulation bacteria, health implications. this review, biology examined light recent literature nearly 4 decades research. Q's neuropsychiatric cancer highlighted discussed. Given interest Q, new findings, more research needed fully comprehend biological function disease relevance, especially neurobiology.
Language: Английский
Citations
5
Advanced Science, Journal Year: 2024, Volume and Issue: 11(44)
Published: Oct. 8, 2024
Abstract RNA‐modifying proteins, classified as “writers,” “erasers,” and “readers,” dynamically modulate RNA by adding, removing, or interpreting chemical groups, thereby influencing stability, functionality, interactions. To date, over 170 distinct modifications more than 100 enzymes have been identified, with ongoing research expanding these numbers. Although significant progress has made in understanding modification, the regulatory mechanisms that govern proteins themselves remain insufficiently explored. Post‐translational (PTMs) such phosphorylation, ubiquitination, acetylation are crucial modulating function behavior of proteins. However, full extent PTM influence on their role disease development remains to be fully elucidated. This review addresses gaps offering a comprehensive analysis roles PTMs play regulating Mechanistic insights provided into how alter biological processes, contribute cellular function, drive progression. In addition, current landscape is examined, highlighting therapeutic potential targeting for precision medicine. By advancing networks, this seeks facilitate effective strategies inspire future critical area
Language: Английский
Citations
4
Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 15
Published: Jan. 9, 2025
N7-methylguanosine (m7G) modification is one of the most prevalent forms chemical in RNA molecules, which plays an important role biological processes such as stability, translation regulation and ribosome recognition. Methyl-transferation m7G catalyzed by enzyme complex methyltransferase-like 1 (METTL1) WD repeat domain 4 (WDR4), Quaking (QKI) recognizes internal methylated mRNA regulates stabilization. Recent studies have found that - related enzymes are associated with onset progression digestive cancer, colorectal liver other diseases ulcerative colitis. This review will focus on latest research progress roles methyltransferase METTL1/WDR4 recognized QKI diseases.
Language: Английский
Citations
0
Wiley Interdisciplinary Reviews - RNA, Journal Year: 2025, Volume and Issue: 16(2)
Published: March 1, 2025
ABSTRACT Transfer RNA (tRNA) is not merely a passive carrier of amino acids, but an active regulator mRNA translation controlling codon bias and optimality. The synthesis various tRNA modifications regulated by many “writer” enzymes, which utilize substrates from metabolic pathways or dietary sources. Metabolic bioenergetic pathways, such as one‐carbon (1C) metabolism the tricarboxylic acid (TCA) cycle produce essential for synthesis, S‐Adenosyl methionine (SAM), sulfur species, α‐ketoglutarate (α‐KG). activity these can directly impact decoding via regulating levels. In this review, we discuss complex interactions between diet, metabolism, modifications, translation. We how nutrient availability, bioenergetics, intermediates modulate modification landscape to fine‐tune protein synthesis. Moreover, highlight dysregulation metabolic‐tRNA contributes disease pathogenesis, including cancer, disorders, neurodegenerative diseases. also new emerging field GlycoRNA biology drawing parallels glycobiology diseases guide future directions in area. Throughout our discussion, links specific their metabolic/dietary precursors, diseases, emphasizing importance metabolism‐centric view understanding pathologies. Future research should focus on uncovering interplay cellular contexts. Addressing gaps will into novel interventions.
Language: Английский
Citations
0
International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 309, P. 142940 - 142940
Published: April 8, 2025
Language: Английский
Citations
0
Gene, Journal Year: 2025, Volume and Issue: 959, P. 149518 - 149518
Published: April 18, 2025
Language: Английский
Citations
0