Clonorchis sinensis-infected hepatocellular carcinoma exhibits distinct tumor microenvironment and molecular features DOI Creative Commons

Junxian Chen,

Caibiao Wei, Wen‐Cheng Huang

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: March 17, 2025

Clonorchis sinensis (Cs)-infected hepatocellular carcinoma (HCC) patients have a poorer prognosis than non-Cs-infected HCCs. However, the molecular mechanisms of Cs-infected HCC remain unclear. To address this, this study aims to uncover tumor microenvironment and features that may contribute these poor outcomes. The research involved bulk RNA sequencing paired adjacent tissue samples from 10 Cs + - patients. Differentially expressed genes were identified, followed by enrichment analyses reveal functional changes. Survival analysis top up- down-regulated in tumors was performed using TCGA database. Additionally, clinical data 1,461 retrospectively analyzed assess impact infection on microvascular invasion metastasis rates. In vitro assays also conducted excretory/secretory products (CsESPs) examine their effect cells HUVECs. We identified 785 up-regulated 675 compared tumors, enriched pathways related extracellular matrix remodeling immunosuppression. revealed are associated with prognosis. Clinical showed increased vitro, CsESPs enhanced migration promoted tube formation human umbilical vein endothelial cells. This provides novel insights into landscape underscores infection's role enhancing migration, angiogenesis. findings understanding parasitic infections cancer progression suggest potential prognostic markers for HCC.

Language: Английский

Clonorchis sinensis-infected hepatocellular carcinoma exhibits distinct tumor microenvironment and molecular features DOI Creative Commons

Junxian Chen,

Caibiao Wei, Wen‐Cheng Huang

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: March 17, 2025

Clonorchis sinensis (Cs)-infected hepatocellular carcinoma (HCC) patients have a poorer prognosis than non-Cs-infected HCCs. However, the molecular mechanisms of Cs-infected HCC remain unclear. To address this, this study aims to uncover tumor microenvironment and features that may contribute these poor outcomes. The research involved bulk RNA sequencing paired adjacent tissue samples from 10 Cs + - patients. Differentially expressed genes were identified, followed by enrichment analyses reveal functional changes. Survival analysis top up- down-regulated in tumors was performed using TCGA database. Additionally, clinical data 1,461 retrospectively analyzed assess impact infection on microvascular invasion metastasis rates. In vitro assays also conducted excretory/secretory products (CsESPs) examine their effect cells HUVECs. We identified 785 up-regulated 675 compared tumors, enriched pathways related extracellular matrix remodeling immunosuppression. revealed are associated with prognosis. Clinical showed increased vitro, CsESPs enhanced migration promoted tube formation human umbilical vein endothelial cells. This provides novel insights into landscape underscores infection's role enhancing migration, angiogenesis. findings understanding parasitic infections cancer progression suggest potential prognostic markers for HCC.

Language: Английский

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