Nascent RNA analyses: tracking transcription and its regulation

Nature Reviews Genetics, Journal Year: 2019, Volume and Issue: 20(12), P. 705 - 723

Published: Aug. 9, 2019

Language: Английский

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Transcription in Living Cells: Molecular Mechanisms of Bursting

Annual Review of Biochemistry, Journal Year: 2020, Volume and Issue: 89(1), P. 189 - 212

Published: March 25, 2020

Transcription in several organisms from certain bacteria to humans has been observed be stochastic nature: toggling between active and inactive states. Periods of nascent RNA synthesis known as bursts represent individual gene activation events which multiple polymerases are initiated. Therefore, bursting is the single locus illustration both repression. Although transcriptional was originally decades ago, only recently have technological advances enabled field begin elucidating regulation at single-locus level. In this review, we focus on how biochemical, genomic, single-cell data describe regulatory steps bursts.

Language: Английский

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What Is a Transcriptional Burst?

Trends in Genetics, Journal Year: 2020, Volume and Issue: 36(4), P. 288 - 297

Published: Feb. 5, 2020

Language: Английский

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Epigenetics and addiction

Current Opinion in Neurobiology, Journal Year: 2019, Volume and Issue: 59, P. 128 - 136

Published: June 27, 2019

Language: Английский

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TOP1 inhibition therapy protects against SARS-CoV-2-induced lethal inflammation

Cell, Journal Year: 2021, Volume and Issue: 184(10), P. 2618 - 2632.e17

Published: March 30, 2021

Language: Английский

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Transgenic mice for in vivo epigenome editing with CRISPR-based systems

Nature Methods, Journal Year: 2021, Volume and Issue: 18(8), P. 965 - 974

Published: Aug. 1, 2021

Language: Английский

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Neurobiological functions of transcriptional enhancers

Nature Neuroscience, Journal Year: 2019, Volume and Issue: 23(1), P. 5 - 14

Published: Nov. 18, 2019

Language: Английский

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Enhancer RNAs predict enhancer–gene regulatory links and are critical for enhancer function in neuronal systems

Nucleic Acids Research, Journal Year: 2020, Volume and Issue: 48(17), P. 9550 - 9570

Published: July 31, 2020

Abstract Genomic enhancer elements regulate gene expression programs important for neuronal fate and function are implicated in brain disease states. Enhancers undergo bidirectional transcription to generate non-coding RNAs (eRNAs). However, eRNA remains controversial. Here, we combined Assay Transposase-Accessible Chromatin using Sequencing (ATAC-Seq) RNA-Seq datasets from three distinct culture systems two activity states, enabling genome-wide identification prediction of putative enhancer–gene pairs based on correlation transcriptional output. Notably, stimulus-dependent preceded mRNA induction, CRISPR-based activation synthesis increased at paired genes, functionally validating predictions. Focusing enhancers surrounding the Fos gene, report that targeted manipulation bidirectionally modulates mRNA, eRNAs directly interact with histone acetyltransferase domain enhancer-linked co-activator CREB-binding protein (CBP). Together, these results highlight unique role regulation demonstrate can be used identify target genes.

Language: Английский

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PRC1 drives Polycomb-mediated gene repression by controlling transcription initiation and burst frequency

Nature Structural & Molecular Biology, Journal Year: 2021, Volume and Issue: 28(10), P. 811 - 824

Published: Oct. 1, 2021

Language: Английский

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RETRACTED: Genomic Decoding of Neuronal Depolarization by Stimulus-Specific NPAS4 Heterodimers

Cell, Journal Year: 2019, Volume and Issue: 179(2), P. 373 - 391.e27

Published: Oct. 1, 2019

Language: Английский

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