Development and organization of the retinal orientation selectivity map DOI Creative Commons
Dominic J. Vita,

Fernanda S. Orsi,

Nathan G. Stanko

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: June 6, 2024

Abstract Orientation or axial selectivity, the property of neurons in visual system to respond preferentially certain angles stimuli, plays a pivotal role our understanding perception and information processing. This computation is performed as early retina, although much work has established cellular mechanisms retinal orientation how this organized across retina unknown. Using large dataset collected mouse we demonstrate functional organization rules selectivity. First, identify three major classes cells that are selective match previous descriptions. Second, show one predominantly represented predominant changes function location. Third, neural activity little on Lastly, use silico modeling followed by validation experiments overrepresented aligns along concentric axes. These results that, similar direction selectivity map retina.

Language: Английский

Evolution of neuronal cell classes and types in the vertebrate retina DOI Creative Commons
Joshua Hahn, Aboozar Monavarfeshani,

Mu Qiao

et al.

Nature, Journal Year: 2023, Volume and Issue: 624(7991), P. 415 - 424

Published: Dec. 13, 2023

Abstract The basic plan of the retina is conserved across vertebrates, yet species differ profoundly in their visual needs 1 . Retinal cell types may have evolved to accommodate these varied needs, but this has not been systematically studied. Here we generated and integrated single-cell transcriptomic atlases from 17 species: humans, two non-human primates, four rodents, three ungulates, opossum, ferret, tree shrew, a bird, reptile, teleost fish lamprey. We found high molecular conservation six retinal classes (photoreceptors, horizontal cells, bipolar amacrine ganglion cells (RGCs) Müller glia), with variation related evolutionary distance. Major subclasses were also conserved, whereas among within or was more pronounced. However, an integrative analysis revealed that numerous are shared species, based on gene expression programmes likely trace back early ancestral vertebrate. degree increased outer (photoreceptors) inner (RGCs), suggesting evolution acts preferentially shape output. Finally, identified rodent orthologues midget RGCs, which comprise than 80% RGCs human retina, subserve high-acuity vision, previously believed be restricted primates 2 By contrast, mouse large receptive fields around 2% RGCs. Projections both primate orthologous overrepresented thalamus, supplies primary cortex. suggest innovations, descendants evolutionarily ancient decreased size number as evolved, thereby facilitating acuity cortical processing information.

Language: Английский

Citations

84
Ancestral photoreceptor diversity as the basis of visual behaviour DOI
Tom Baden

Nature Ecology & Evolution, Journal Year: 2024, Volume and Issue: 8(3), P. 374 - 386

Published: Jan. 22, 2024

Language: Английский

Citations

16
Transcriptomic neuron types vary topographically in function and morphology DOI Creative Commons
Inbal Shainer, Johannes M. Kappel,

Eva Laurell

et al.

Nature, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 12, 2025

Abstract Neuronal phenotypic traits such as morphology, connectivity and function are dictated, to a large extent, by specific combination of differentially expressed genes. Clusters neurons in transcriptomic space correspond distinct cell types some cases—for example, Caenorhabditis elegans 1 retinal ganglion cells 2–4 —have been shown share morphology function. The zebrafish optic tectum is composed spatial array that transforms visual inputs into motor outputs. Although the visuotopic map continuous, subregions functionally specialized 5,6 . Here, uncover cell-type architecture tectum, we transcriptionally profiled its neurons, revealing more than 60 organized anatomical layers. We measured responses thousands tectal two-photon calcium imaging matched them with their transcriptional profiles. Furthermore, characterized morphologies identified using transgenic lines. Notably, found similar can diverge shape, responses. Incorporating coordinates within volume revealed morphologically defined subclusters individual clusters. Our findings demonstrate extrinsic, position-dependent factors expand repertoire genetically neurons.

Language: Английский

Citations

2
Diversification of multipotential postmitotic mouse retinal ganglion cell precursors into discrete types DOI Creative Commons
Karthik Shekhar,

Irene E. Whitney,

Salwan Butrus

et al.

eLife, Journal Year: 2022, Volume and Issue: 11

Published: Feb. 22, 2022

The genesis of broad neuronal classes from multipotential neural progenitor cells has been extensively studied, but less is known about the diversification a single class into multiple types. We used single-cell RNA-seq to study how newly born (postmitotic) mouse retinal ganglion cell (RGC) precursors diversify ~45 discrete Computational analysis provides evidence that RGC transcriptomic type identity not specified at mitotic exit, acquired by gradual, asynchronous restriction postmitotic precursors. Some types are identifiable until week after they generated. Immature RGCs may be project ipsilaterally or contralaterally rest brain before their emerges. Optimal transport inference identifies groups with largely nonoverlapping fates, distinguished selectively expressed transcription factors could act as fate determinants. Our framework for investigating molecular within class.

Language: Английский

Citations

56
Origins of direction selectivity in the primate retina DOI Creative Commons
Yeon Jin Kim,

Beth B. Peterson,

Joanna D. Crook

et al.

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: May 23, 2022

From mouse to primate, there is a striking discontinuity in our current understanding of the neural coding motion direction. In non-primate mammals, directionally selective cell types and circuits are signature feature retina, situated at earliest stage visual process. primates, by contrast, direction selectivity hallmark processing areas cortex, but has not been found despite significant effort. Here we combined functional recordings light-evoked responses connectomic reconstruction identify diverse direction-selective macaque monkey retina with distinctive physiological properties synaptic motifs. This circuitry includes an ON-OFF ganglion type, spiking, polyaxonal amacrine starburst cell, all which show selectivity. Moreover, discovered that cells possess strong, non-GABAergic, antagonistic surround mediated input from excitatory bipolar critical for generation radial sensitivity these cells. Our findings open door investigation precortical computes primate system.

Language: Английский

Citations

50
Retinal ganglion cell repopulation for vision restoration in optic neuropathy: a roadmap from the RReSTORe Consortium DOI Creative Commons
Jonathan R. Soucy,

Erika A. Aguzzi,

Julie Cho

et al.

Molecular Neurodegeneration, Journal Year: 2023, Volume and Issue: 18(1)

Published: Sept. 21, 2023

Retinal ganglion cell (RGC) death in glaucoma and other optic neuropathies results irreversible vision loss due to the mammalian central nervous system's limited regenerative capacity. RGC repopulation is a promising therapeutic approach reverse from if newly introduced neurons can reestablish functional retinal thalamic circuits. In theory, RGCs might be repopulated through transplantation of stem cell-derived or via induction endogenous transdifferentiation. The Repopulation, Stem Cell Transplantation, Optic Nerve Regeneration (RReSTORe) Consortium was established address challenges associated with repair visual pathway neuropathy. 2022, RReSTORe initiated ongoing international collaborative discussions advance field has identified five critical areas focus: (1) development differentiation, (2) Transplantation methods models, (3) survival, maturation, host interactions, (4) Inner wiring, (5) Eye-to-brain connectivity. Here, we discuss most pertinent questions that exist on path clinical translation suggest experimental directions propel this work going forward. Using these subtopic discussion groups (SDGs) as framework, multidisciplinary approaches restore diseased by leveraging groundbreaking insights developmental neuroscience, biology, molecular optical imaging, animal models neuropathy, immunology & immunotolerance, neuropathology neuroprotection, materials science biomedical engineering, neuroscience. While significant hurdles remain, Consortium's efforts provide comprehensive roadmap for advancing hold potential transformative progress restoring patients suffering neuropathies.

Language: Английский

Citations

31
From water to land: Evolution of photoreceptor circuits for vision in air DOI Creative Commons
Tom Baden

PLoS Biology, Journal Year: 2024, Volume and Issue: 22(1), P. e3002422 - e3002422

Published: Jan. 22, 2024

When vertebrates first conquered the land, they encountered a visual world that was radically distinct from of their aquatic ancestors. Fish exploit strong wavelength-dependent interactions light with water by differentially feeding signals up to 5 spectral photoreceptor types into behavioural programmes. However, above same rules do not apply, and this called for an update circuit strategies. Early tetrapods soon evolved double cone, still poorly understood pair new photoreceptors brought “ancestral terrestrial” complement 7. Subsequent nonmammalian lineages adapted highly parallelised retinal input strategy diverse ecologies. By contrast, mammals shed most ancestral converged on is exceptionally general. In eutherian including in humans, parallelisation emerges gradually as signal traverses layers retina brain.

Language: Английский

Citations

12
Perivascular neurons instruct 3D vascular lattice formation via neurovascular contact DOI

Kenichi Toma,

Mengya Zhao, Shaobo Zhang

et al.

Cell, Journal Year: 2024, Volume and Issue: 187(11), P. 2767 - 2784.e23

Published: May 1, 2024

Language: Английский

Citations

12
Parallel pathways carrying direction-and orientation-selective retinal signals to layer 4 of the mouse visual cortex DOI Creative Commons
Helen Wang,

Oyshi Dey,

Willian N. Lagos

et al.

Cell Reports, Journal Year: 2024, Volume and Issue: 43(3), P. 113830 - 113830

Published: Feb. 21, 2024

Language: Английский

Citations

9
A chromatic feature detector in the retina signals visual context changes DOI Creative Commons
Larissa Höfling, Klaudia P. Szatko, Christian Behrens

et al.

eLife, Journal Year: 2024, Volume and Issue: 13

Published: Oct. 4, 2024

The retina transforms patterns of light into visual feature representations supporting behaviour. These are distributed across various types retinal ganglion cells (RGCs), whose spatial and temporal tuning properties have been studied extensively in many model organisms, including the mouse. However, it has difficult to link potentially nonlinear transformations natural inputs specific ethological purposes. Here, we discover a selectivity chromatic contrast an RGC type that allows detection changes context. We trained convolutional neural network (CNN) on large-scale functional recordings responses mouse movies, then used this search silico for stimuli maximally excite distinct RGCs. This procedure predicted centre colour opponency transient suppressed-by-contrast (tSbC) RGCs, cell function is being debated. confirmed experimentally these indeed responded very selectively Green-OFF, UV-ON contrasts. was characteristic transitions from ground sky scene, as might be elicited by head or eye movements horizon. Because tSbC performed best among all at reliably detecting transitions, suggest role providing contextual information (i.e. ground) necessary selection appropriate behavioural other stimuli, such looming objects. Our work showcases how combination experiments with computational modelling discovering novel stimulus identifying their potential relevance.

Language: Английский

Citations

9