Role of SYT11 in human pan-cancer using comprehensive approaches DOI Creative Commons

Kyunghee Noh,

Hyunji Choi, Eunhye Jo

et al.

European journal of medical research, Journal Year: 2024, Volume and Issue: 29(1)

Published: June 18, 2024

Abstract Background Synaptotagmin 11 (SYT11) plays a pivotal role in neuronal vesicular trafficking and exocytosis. However, no independent prognostic studies have focused on various cancers. In this study, we aimed to summarize the clinical significance molecular landscape of SYT11 tumor types. Methods Using several available public databases, investigated abnormal expression different types its potential association with prognosis, methylation profiling, immune infiltration, gene enrichment analysis, protein–protein interaction identified common pathways. Results TCGA Genotype-Tissue Expression (GTEx) showed that was widely expressed across corresponding normal tissues. Survival analysis correlated prognosis seven cancer Additionally, mRNA not affected by promoter methylation, but regulated certain miRNAs associated patient prognosis. vitro experiments further verified negative correlation between miR-19a-3p human colorectal, lung, renal cell lines. Moreover, aberrant significantly infiltration. Pathway revealed biological processes were related clathrin-mediated endocytosis, Rho GTPase signaling, motility-related functions. Conclusions Our results provide clear understanding suggest may be significance.

Language: Английский

Clinicopathological features and prognostic significance of SYT8 in patients with colorectal cancer who received curative surgery DOI Open Access

Jin Zhu,

Jiarong Shang, Yuan Li

et al.

Gastrointestinal Tumors, Journal Year: 2025, Volume and Issue: 12(1), P. 0 - 0

Published: Jan. 1, 2025

Language: Английский

Citations

0
Synaptotagmin-11 facilitates assembly of a presynaptic signaling complex in post-Golgi cargo vesicles DOI Creative Commons
Luca Trovò, Stylianos Kouvaros, Jochen Schwenk

et al.

EMBO Reports, Journal Year: 2024, Volume and Issue: 25(6), P. 2610 - 2634

Published: May 2, 2024

Abstract GABA B receptors (GBRs), the G protein-coupled for GABA, regulate synaptic transmission throughout brain. A main function of GBRs is gating Cav2.2-type Ca 2+ channels. However, cellular compartment where stable GBR/Cav2.2 signaling complexes form remains unknown. In this study, we demonstrate that vesicular protein synaptotagmin-11 (Syt11) binds to both auxiliary GBR subunit KCTD16 and Cav2.2 Through these dual interactions, Syt11 recruits channels post-Golgi vesicles, thus facilitating assembly complexes. addition, stabilizes at neuronal plasma membrane by inhibiting constitutive internalization. Neurons knockout mice exhibit deficits in presynaptic channels, reduced neurotransmitter release, decreased GBR-mediated inhibition, highlighting critical role expression These findings support acts as a scaffold protein, aiding from low-abundance components within transport vesicles. This mechanism enables insertion pre-assembled functional units into membrane.

Language: Английский

Citations

2
Toxicity of microplastic fibers containing azobenzene disperse dyes to human lung epithelial cells cultured at an air-liquid interface DOI Creative Commons

Amber O’Connor,

Anna Villalobos Santeli,

Sripriya Nannu Shankar

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 480, P. 136280 - 136280

Published: Oct. 30, 2024

Language: Английский

Citations

2
Characterizing the secretome of EGFR mutant lung adenocarcinoma DOI Creative Commons

Jennifer Luu,

Fraser Johnson,

Jana M. Jajarmi

et al.

Frontiers in Oncology, Journal Year: 2024, Volume and Issue: 13

Published: Jan. 8, 2024

Background Lung cancer is the leading cause of related death worldwide, mainly due to late stage disease at time diagnosis. Non-invasive biomarkers are needed supplement existing screening methods enable earlier detection and increased patient survival. This critical EGFR -driven lung adenocarcinoma as it commonly occurs in individuals who have never smoked do not qualify for current protocols. Methods In this study, we performed mass spectrometry analysis secretome cultured cells representing different stages mutant driven transformation, from normal fully malignant. Identified secreted proteins specific malignant state were validated using orthogonal their clinical activity assessed cohorts. Results We quantified 1020 proteins, which compared differential expression between transformation. differentially expressed transcriptional level tumor specimens, association with survival, absolute concentration yield three biomarker candidates: MDK, GDF15, SPINT2. These candidates ELISA levels associated poor survival specifically patients. Conclusions Our study provides insight into changes during transformation that may play a role processes promote progression. The identified can harnessed use identify high risk early programs management molecular subgroup

Language: Английский

Citations

1
PI4K2A: a novel potential therapeutic target for lung cancer DOI Open Access
Camila Machado Baldavira, Tabatha Gutierrez Prieto, María Aparecida Nagai

et al.

Translational Lung Cancer Research, Journal Year: 2024, Volume and Issue: 13(1), P. 199 - 204

Published: Jan. 1, 2024

Language: Английский

Citations

1
Constructing a Novel Amino Acid Metabolism Signature: A New Perspective on Pheochromocytoma Diagnosis, Immune Landscape, and Immunotherapy DOI Creative Commons

Zechen Yan,

Yongkun Luan,

Yu Wang

et al.

Biochemical Genetics, Journal Year: 2024, Volume and Issue: unknown

Published: March 25, 2024

Abstract Pheochromocytoma/paraganglioma (PGPG) is a rare neuroendocrine tumor. Amino acid metabolism crucial for energy production, redox balance, and metabolic pathways in tumor cell proliferation. This study aimed to build risk model using amino metabolism-related genes, enhancing PGPG diagnosis treatment decisions. We analyzed RNA-sequencing data from the PCPG cohort GEO dataset as our training set validated findings TCGA an additional clinical cohort. WGCNA LASSO were utilized identify hub genes develop prediction models. The single-sample gene enrichment analysis, MCPCOUNTER, ESTIMATE algorithm calculated relationship between immune infiltration PCPG. TIDE predicted immunotherapy efficacy patients. analysis identified 292 with differential expression, which are involved pathways. Six (DDC, SYT11, GCLM, PSMB7, TYRO3, AGMAT) model. Patients high-risk profile demonstrated reduced but potentially higher benefits immunotherapy. Notably, DDC SYT11 showed strong diagnostic prognostic potential. Validation through quantitative Real-Time Polymerase Chain Reaction immunohistochemistry confirmed their underscoring significance predicting response. study’s integration of into offers critical insights stratification, potential responses, drug development, planning, marking significant step forward management this complex condition.

Language: Английский

Citations

1
Role of SYT11 in human pan-cancer using comprehensive approaches DOI Creative Commons

Kyunghee Noh,

Wonbeak Yoo, Kyung Chan Park

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 2, 2024

Abstract Background Synaptotagmin 11 (SYT11) plays a pivotal role in neuronal vesicular trafficking and exocytosis. However, no independent prognostic studies have focused on various cancers. In this study, we aimed to summarize the clinical significance molecular landscape of SYT11 tumor types. Methods Using several available public databases, investigated abnormal expression different types its potential association with prognosis, methylation profiling, immune infiltration, gene enrichment analysis, protein–protein interaction identified common pathways. Results TCGA Genotype-Tissue Expression (GTEx) showed that was widely expressed across corresponding normal tissues. Survival analysis correlated prognosis seven cancer Additionally, mRNA not affected by promoter methylation, but regulated certain miRNAs associated patient prognosis. Moreover, aberrant significantly infiltration. Pathway revealed biological processes were related clathrin-mediated endocytosis, Rho GTPase signaling, cell motility-related functions. Conclusions Our results provide clear understanding suggest may be significance.

Language: Английский

Citations

0
Role of SYT11 in human pan-cancer using comprehensive approaches DOI Creative Commons

Kyunghee Noh,

Hyunji Choi, Eunhye Jo

et al.

European journal of medical research, Journal Year: 2024, Volume and Issue: 29(1)

Published: June 18, 2024

Abstract Background Synaptotagmin 11 (SYT11) plays a pivotal role in neuronal vesicular trafficking and exocytosis. However, no independent prognostic studies have focused on various cancers. In this study, we aimed to summarize the clinical significance molecular landscape of SYT11 tumor types. Methods Using several available public databases, investigated abnormal expression different types its potential association with prognosis, methylation profiling, immune infiltration, gene enrichment analysis, protein–protein interaction identified common pathways. Results TCGA Genotype-Tissue Expression (GTEx) showed that was widely expressed across corresponding normal tissues. Survival analysis correlated prognosis seven cancer Additionally, mRNA not affected by promoter methylation, but regulated certain miRNAs associated patient prognosis. vitro experiments further verified negative correlation between miR-19a-3p human colorectal, lung, renal cell lines. Moreover, aberrant significantly infiltration. Pathway revealed biological processes were related clathrin-mediated endocytosis, Rho GTPase signaling, motility-related functions. Conclusions Our results provide clear understanding suggest may be significance.

Language: Английский

Citations

0