Genomic analyses provide insights into the genome evolution and environmental adaptation of the tobacco moth Ephestia elutella DOI Creative Commons

Jiadan Xu,

Bo Li, Zhimin Jiang

et al.

Frontiers in Physiology, Journal Year: 2023, Volume and Issue: 14

Published: April 19, 2023

Ephestia elutella is a major pest responsible for significant damage to stored tobacco over many years. Here, we conduct comparative genomic analysis on this pest, aiming explore the genetic bases of environmental adaptation species. We find gene families associated with nutrient metabolism, detoxification, antioxidant defense and gustatory receptors are expanded in E. genome. Detailed phylogenetic P450 genes further reveals obvious duplications CYP3 clan compared closely related species, Indianmeal moth Plodia interpunctella . also identify 229 rapidly evolving 207 positively selected , respectively, highlight two heat shock protein 40 ( Hsp40 ) genes. In addition, number species-specific diverse biological processes, such as mitochondria biology development. These findings advance our understanding mechanisms underlying processes will enable development novel management strategies.

Language: Английский

Genomes of the cosmopolitan fruit pest Bactrocera dorsalis (Diptera: Tephritidae) reveal its global invasion history and thermal adaptation DOI Creative Commons
Yue Zhang, Shanlin Liu, Marc De Meyer

et al.

Journal of Advanced Research, Journal Year: 2022, Volume and Issue: 53, P. 61 - 74

Published: Dec. 24, 2022

The oriental fruit fly Bactrocera dorsalis is one of the most destructive agricultural pests worldwide, with highly debated species delimitation, origin, and global spread routes.Our study intended to (i) resolve taxonomic uncertainties between B. carambolae, (ii) reveal population structure invasion routes across Asia, Africa, Oceania, (iii) identify genomic regions that are responsible for thermal adaptation dorsalis.Based on a high-quality chromosome-level reference genome assembly, we explored relationship using genome-scale single nucleotide polymorphism dataset generated from resequencing data 487 genomes 25 carambolae genomes. Genome-wide association studies silencing RNA interference were used verify candidate genes associated extreme stress.We showed originates Southern India region three independent worldwide: Northern Southeast then Asia; Asian, China Hawaii; toward African mainland, Madagascar, which mainly facilitated by human activities including trade immigration. Twenty-seven identified genome-wide be 11 temperature bioclimatic variables. Cyp6a9 gene may enhance thus boost its invasion, tended upregulated at hardening 38 °C. Functional verification against Cyp6a9, led specific decrease in expression, reducing survival rate dsRNA-feeding larvae exposed stress 45 °C after heat treatments dorsalis.This provides insights into evolutionary history genetic basis dorsalis.

Language: Английский

Citations

36
Opsin mutants alter host plant selection by color vision in the nocturnal invasive pest Tuta absoluta DOI Creative Commons

Yanhong Tang,

Siyan Bi,

Xiaodi Wang

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 265, P. 130636 - 130636

Published: March 11, 2024

In insects, vision is crucial in finding host plants, but its role nocturnal insects largely unknown. Vision involves responses to specific spectra of photon wavelengths and opsins plays an important this process. Long-wavelength sensitive opsin (LW opsin) blue-sensitive (BL are main visual proteins play behavior regulation.We used CRISPR/Cas9 technology mutate the long-wavelength-sensitive blue wavelength-sensitive genes explored invasive pest Tuta absoluta. Light wave experiments revealed that LW2 (−/−) BL(−/−) mutants showed abnormal wavelength tropism. Both BL mutations affected preference T. absoluta for green environment. Mutations necessary inhibit attraction. The elimination leaf moths both induced a white plants. Behavioral changes resulting from LW2(−/−) were not by sense smell, further supporting regulatory insect behavior. To best our knowledge, first study reveal vision, host-seeking at night, which key factors. These findings will drive development "vision-ecology" theory.

Language: Английский

Citations

4
The Genomics Revolution Drives a New Era in Entomology DOI
Fei Li, Xianhui Wang, Xin Zhou

et al.

Annual Review of Entomology, Journal Year: 2025, Volume and Issue: 70(1), P. 379 - 400

Published: Jan. 28, 2025

Thanks to the fast development of sequencing techniques and bioinformatics tools, genome an insect species for specific research purposes has become increasingly popular practice. Insect genomes not only provide sets gene sequences but also represent a change in focus from reductionism systemic biology field entomology. Using genomes, researchers are able identify study functions all members family, pathway, or network associated with trait interest. Comparative genomics studies new insights into evolution, addressing long-lasting controversies taxonomy. It is now feasible uncover genetic basis important traits by identifying variants using resequencing data individual insects, followed genome-wide association analysis. Here, we review current progress projects application uncovering phylogenetic relationships between insects unraveling mechanisms life-history traits. We summarize challenges sharing possible solutions. Finally, guidance fully deeply mining data.

Language: Английский

Citations

0
Specific transcription factors regulate the expression of Rh6 in Bactrocera minax and Bactrocera dorsalis (Diptera: Tephritidae) DOI
Haoran Wang, Guijian Zhang, Yaohui Wang

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 141201 - 141201

Published: Feb. 1, 2025

Language: Английский

Citations

0
Transcriptomic analyses in thirteen Tephritidae species provide insights into the ecological driving force behind odorant receptor evolution DOI Creative Commons
Emma Persyn, Pierre François Duyck,

Marie-Christine François

et al.

Molecular Phylogenetics and Evolution, Journal Year: 2025, Volume and Issue: unknown, P. 108322 - 108322

Published: March 1, 2025

Language: Английский

Citations

0
Chromosome‐Level Genome Assembly of the Leafcutter Bee Megachile Rotundata Reveals Its Ecological Adaptation and Pollination Biology DOI Creative Commons

Rangjun Shi,

Peng Duan,

Meng-meng Zhu

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

The leafcutter bee Megachile rotundata is the world's most intensively managed solitary bee, owing to its easy manipulation and high pollination efficacy. Here, a high-quality chromosome-level M. genome, covering 280.68 Mb presented. A total of 10 701 genes are predicted, which 93.06% functionally annotated. Based on new genome assembly, transposable elements, noncoding RNAs, as well gene families associated with biology ecological adaptation systematically characterized. Comparative genomic analysis shows notable expansion Toll family but contraction detoxification in genome. Surprisingly, these expanded Toll-1 their downstream display abundant mRNA levels diapausing prepupae. Additionally, prepupae show significantly upregulated expression antimicrobial peptide higher survival rate after Escherichia coli exposure compared nondiapausing prepupae, indicating an enhanced immune response during diapause. provides important foundation for understanding optimizing exceptional efficiency future.

Language: Английский

Citations

0
Evaluation of the Attractant Effect of Solanum muricatum (Solanales: Solanaceae) on Gravid Female Adults of Zeugodacus tau (Diptera: Tephritidae) and Screening of Attractant Volatiles DOI Creative Commons

Ping-Fan Jia,

Xiaoyu Zhang,

Bó Wáng

et al.

Insects, Journal Year: 2023, Volume and Issue: 14(7), P. 591 - 591

Published: June 30, 2023

Zeugodacus tau (Walker) (Diptera: Tephritidae) is a pest seriously harmful to Solanaceae crops and was found oviposit on the pepino melon Solanum muricatum (Aiton). To date, differences in ability of fruits S. other attract gravid Z. females have seldom been reported. Oviposition trapping bioassays were performed clarify whether such existed. A combination GC-MS Y-tube olfactometer system used identify determine compounds inducing behavioral responses volatile odors muricatum. The results show that play role attracting females. influence their these organisms. nine from volatiles induce tendency or repellency Hexyl acetate, butyl amyl actate, isoamyl acetate caused behavior females, while hexyl hexanoate, isovalerate, valerate, hexanoate behavior. Heptyl at higher concentrations (5 mg/mL) but low concentration (0.5 mg/mL). These suggest vigilance against harm by required during cultivation could help develop attractants repellents for are beneficial preventing harming muricatum, developing establishing ecological control

Language: Английский

Citations

4
A neural m6A pathway regulates behavioral aggregation in migratory locusts DOI
Xianliang Huang, Qing Li,

Yanan Xu

et al.

Science China Life Sciences, Journal Year: 2024, Volume and Issue: 67(6), P. 1242 - 1254

Published: March 7, 2024

Language: Английский

Citations

1
A ghost moth olfactory prototype of the lepidopteran sex communication DOI Creative Commons
Rui Tang, Cong Huang, Jun Yang

et al.

GigaScience, Journal Year: 2024, Volume and Issue: 13

Published: Jan. 1, 2024

Abstract Sex role differentiation is a widespread phenomenon. pheromones are often associated with sex roles and convey sex-specific information. In Lepidoptera, females release to attract males, which evolve sophisticated olfactory structures relay pheromone signals. However, in some primitive moths, becomes diverged. Here, we introduce the chromosome-level genome assembly from ancestral Himalaya ghost revealing unique evolution pattern parity among Lepidoptera. These of moths characterized by dense population trichoid sensilla, both larger male female antennal entry parts brains, compared evolutionary later Furthermore, tandem 34 odorant receptor 19 homologs Thitarodes xiaojinensis (TxiaOr19) has been identified, presents overlapped motifs receptors (PRs). Interestingly, expanded TxiaOr19 was predicted have unconventional tuning patterns canonical PRs, nonsexual dimorphic neuropils discovered, contributes observed equal adults. Additionally, transposable element activity bursts provided traceable loci landscapes where parallel diversifications occurred between indicating that Or19 homolog expansions were diversified PRs during thus established classic higher moths. This study elucidates an prototype intermediate communication

Language: Английский

Citations

1
Attraction of Nicotiana benthamiana to Bemisia tabaci is related to a chemical signal in plant volatile, undecane DOI
Xinyue Zhang, Wen‐Hao Han, Fengbin Zhang

et al.

Pest Management Science, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 11, 2024

Abstract BACKGROUND The whitefly Bemisia tabaci is one of the world's foremost agricultural pests. Recently, we found that a wild relative tobacco ( Nicotiana benthamiana ) demonstrates remarkable attractiveness and nearly 100% lethality towards whiteflies. Therefore, it can act as dead‐end trap crop for control in field. However, underlying mechanism significant N. whiteflies unclear. RESULTS Binary‐choice assays olfactory experiments showed compared to common tabacum ), volatile has greater attraction Then analyzed volatiles from these two species by thermal desorption‐gas chromatography–mass spectrometry (TD‐GC–MS). We identified 16 chemical compounds are more abundant than tabacum. Seven were further tested with olfactometer that, among them, undecane strongly attracted Further revealed even 0.005 μg mL −1 attractive also silenced genes may influence biosynthesis production decreased after silencing NbCER3 , plants less lost their In addition, applying on yellow sticky traps increase number stuck insects ≈40%. CONCLUSION Undecane critical signal attracts involved undecane. be used improve efficiency control. © 2024 Society Chemical Industry.

Language: Английский

Citations

1