Regional deficits in endogenous regeneration of mouse olfactory sensory neuron axons DOI Creative Commons
Tenzin Kunkhyen, Kendall A. Curtis,

Thomas P. Deakin

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 7, 2024

Abstract Postnatal neurogenesis occurs in only a few regions of the mammalian nervous system. Hence, neurons that are lost due to neurodegenerative disease, stroke, traumatic brain injury or peripheral neuropathy cannot be replaced. Transplantation stem cell-derived provides potential replacement strategy, but how these can encouraged functionally integrate into circuits remains significant challenge. In olfactory epithelium (OE), sensory (OSNs) continue generated throughout life from basal cells and repopulated even after complete ablation. However, specialized population navigator OSNs ensures accurate odorant receptor-specific targeting OSN axons glomeruli bulb (OB) is present perinatally. Despite this, some studies have reported regeneration specific glomeruli, while others found various degrees recovery, following cell death. Variability extent both initial ablation subsequent repopulation OE, focus on anatomical leave which newly reinnervate OB unclear. Here, we employed olfactotoxic drug methimazole selectively ablate without damaging generate them, enabling us assess functional recovery input context repopulation. We profound deficits odor-evoked responses glomerular layer dorsal five weeks ablation, time point at known OE. Histological analysis mature 10 20 post-methimazole showed persistent region-specific deficit axon reinnervation OB, with dorsomedial region being particularly adversely affected. contrast, ventral, lateral medial was almost by post-MMZ. identified mouse sets stage identify mechanisms mediate successful vs. unsuccessful axonal an endogenous neurons.

Language: Английский

Cell type- and layer-specific plasticity of olfactory bulb interneurons following olfactory sensory neuron ablation DOI Creative Commons
Tenzin Kunkhyen,

Taryn R. Brechbill,

Sarah P. R. Berg

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Aug. 1, 2024

Lifelong neurogenesis endows the mouse olfactory system with a capacity for regeneration that is unique in mammalian nervous system. Throughout life, sensory neurons (OSNs) are generated from epithelium (OE) stem cells nose, while subventricular zone generates neuroblasts migrate to bulb (OB) and differentiate into multiple populations of inhibitory interneurons. Methimazole (MMZ) selectively ablates OSNs, but OE enables OSN repopulation gradual recovery input OB within 6 weeks. However, it not known how interneurons affected by this loss subsequent following MMZ treatment. We found dopaminergic neuron density was significantly reduced 7–14 days post-MMZ recovered substantially at 35 days. The parvalbumin-expressing unaffected MMZ; however, their soma size post-MMZ, recovering Surprisingly, we transient increase calretinin-expressing glomerular external plexiform layers, granule cell layer, 7 post-MMZ. This could be accounted increased may result calretinin expression. Together, our data demonstrate type- layer-specific changes interneuron morphology after treatment, providing new insight range plasticity mechanisms employed circuits during input.

Language: Английский

Citations

3
Roles of odorant receptors during olfactory glomerular map formation DOI Creative Commons
Ai Nakashima, Haruki Takeuchi

genesis, Journal Year: 2024, Volume and Issue: 62(3)

Published: June 1, 2024

Summary The organization of the olfactory glomerular map involves convergence sensory neurons (OSNs) expressing same odorant receptor (OR) into glomeruli in bulb (OB). A remarkable feature formation is that identity OR instructs topography bulb, resulting thousands discrete mice. Several lines evidence indicate ORs control expression levels various kinds transmembrane proteins to form at appropriate regions OB. In this review, we will discuss how decoded by OSNs gene through intracellular regulatory mechanisms.

Language: Английский

Citations

2
Cell type- and layer-specific plasticity of olfactory bulb interneurons following olfactory sensory neuron ablation DOI Creative Commons
Tenzin Kunkhyen,

Taryn R. Brechbill,

Sarah P. R. Berg

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 9, 2024

Abstract Lifelong neurogenesis endows the mouse olfactory system with a capacity for regeneration that is unique in mammalian nervous system. Throughout life, sensory neurons (OSNs) are generated from epithelium (OE) stem cells nose, while subventricular zone generates neuroblasts migrate to bulb (OB) and differentiate into multiple populations of inhibitory interneurons. Methimazole (MMZ) selectively ablates OSNs, but OE enables OSN repopulation gradual recovery input OB within six weeks. However, it not known how interneurons affected by this loss subsequent following MMZ treatment. We found dopaminergic neuron density was significantly reduced 7-14 days post-MMZ recovered substantially at 35 days. The parvalbumin-expressing unaffected MMZ; however, their soma size post-MMZ, recovering Surprisingly, we transient increase calretinin-expressing glomerular external plexiform layers, granule cell layer, 7 post-MMZ. This could be accounted increased may result calretinin expression. At time points, all three layers showed 14 baseline Together, our data demonstrate type- layer-specific changes interneuron morphology after treatment, providing new insight range plasticity mechanisms employed circuits during input.

Language: Английский

Citations

0
Regional deficits in endogenous regeneration of mouse olfactory sensory neuron axons DOI Creative Commons
Tenzin Kunkhyen, Kendall A. Curtis,

Thomas P. Deakin

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 7, 2024

Abstract Postnatal neurogenesis occurs in only a few regions of the mammalian nervous system. Hence, neurons that are lost due to neurodegenerative disease, stroke, traumatic brain injury or peripheral neuropathy cannot be replaced. Transplantation stem cell-derived provides potential replacement strategy, but how these can encouraged functionally integrate into circuits remains significant challenge. In olfactory epithelium (OE), sensory (OSNs) continue generated throughout life from basal cells and repopulated even after complete ablation. However, specialized population navigator OSNs ensures accurate odorant receptor-specific targeting OSN axons glomeruli bulb (OB) is present perinatally. Despite this, some studies have reported regeneration specific glomeruli, while others found various degrees recovery, following cell death. Variability extent both initial ablation subsequent repopulation OE, focus on anatomical leave which newly reinnervate OB unclear. Here, we employed olfactotoxic drug methimazole selectively ablate without damaging generate them, enabling us assess functional recovery input context repopulation. We profound deficits odor-evoked responses glomerular layer dorsal five weeks ablation, time point at known OE. Histological analysis mature 10 20 post-methimazole showed persistent region-specific deficit axon reinnervation OB, with dorsomedial region being particularly adversely affected. contrast, ventral, lateral medial was almost by post-MMZ. identified mouse sets stage identify mechanisms mediate successful vs. unsuccessful axonal an endogenous neurons.

Language: Английский

Citations

0