Viruses,
Journal Year:
2024,
Volume and Issue:
16(9), P. 1481 - 1481
Published: Sept. 18, 2024
The
inactivated
COVID-19
vaccine
has
demonstrated
high
efficacy
in
the
general
population
through
extensive
clinical
and
real-world
studies.
However,
its
effectiveness
immunocompromised
individuals,
particularly
those
living
with
HIV
(PLWH),
remains
limited.
In
this
study,
20
PLWH
15
HIV-seronegative
individuals
were
recruited
to
evaluate
immunogenicity
of
an
a
prospective
cohort
study.
median
age
was
42
years
31
years,
respectively.
Of
PLWH,
nine
had
been
on
ART
for
over
five
years.
anti-SARS-CoV-2
S-RBD
IgG
antibody
level
d
Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Feb. 1, 2024
Abstract
Dengue
virus-induced
humoral
immunity
can
enhance
the
risk
of
severe
disease,
but
factors
influencing
this
response
are
poorly
understood.
Here,
we
investigated
contribution
CD4
+
T-cells
in
driving
B-cell
human
dengue-infection.
We
identified
a
dominant
peripheral
PD1
T-cell
subset
that
aberrantly
accumulated
patients
and
induce
differentiation
via
utilizing
IL21
help-axis.
Single-cell
analyses
uncovered
heterogeneity
cells
revealing
co-existence
subsets
with
‘helper’
(IL21
)
or
‘cytotoxic’
characteristics.
The
displayed
distinct
clonotypic
transcriptomic
signature
than
Tfh
persist
as
memory
lymph-nodes.
Notably,
show
existence
extrafollicular
responses
dengue
seems
to
controlled
by
-subset.
Our
study
establishes
-subset
potential
determinant
DENV.
These
findings
provide
important
insights
into
T-cell-dependent
regulation
inform
design
therapeutics
effective
vaccines.
One
Sentence
Summary:
Peripheral
T
helper
is
major
development
virus
infection.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: May 10, 2023
Abstract
CD4
+
T
cells
are
a
key
mediator
of
various
autoimmune
diseases;
however,
how
they
contribute
to
disease
development
remains
obscure
primarily
because
their
cellular
heterogeneity.
Here,
we
evaluated
cell
subpopulations
by
decomposition-based
transcriptome
characterization
together
with
canonical
clustering
strategies.
This
approach
identified
12
independent
transcriptional
gene
programs
governing
whole
heterogeneity,
which
can
explain
the
ambiguity
clustering.
In
addition,
performed
meta-analysis
using
public
single-cell
data
sets
over
1.8M
peripheral
from
953
individuals
projecting
onto
reference
and
cataloged
frequency
qualitative
alterations
populations
in
20
diseases.
The
analyses
revealed
that
were
useful
characterizing
each
predicting
its
clinical
status.
Moreover,
genetic
variants
associated
diseases
showed
disease-specific
enrichment
within
programs.
results
collectively
provide
landscape
transcriptomes
involved
disease.
Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Jan. 25, 2024
Abstract
Resting
memory
B-cells
can
be
divided
into
classical
and
non-classical
groups
based
on
differential
expression
of
markers
such
as
CD27
CD11c,
while
activated
express
a
combination
markers,
making
their
ontogeny
hard
to
determine.
Here
by
longitudinal
analysis
COVID-19,
bacterial
sepsis,
BNT162b2
mRNA
vaccine
recipients
mass
cytometry
CITE-seq
we
describe
three-branch
structure
resting
B-cell
consisting
“classical”
CD45RB+
two
branches
CD45RBlo
memory
further
defined
CD23
CD11c
respectively.
Stable
differences
in
CD45RB
upon
activation
allowed
tracking
plasmablasts
derived
from
CD45RBlo
B-cells.
In
both
COVID-19
patients
vaccination,
formed
the
majority
SARS-CoV2
specific
correlated
with
serum
antibodies
was
most
strongly
Sepsis.
These
results
suggest
that
diverse
CD11c+Tbet+
and
CD23+
fractions
form
critical
part
responses
viral
infection
vaccination.
Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: May 2, 2024
Abstract
Regulatory
T
cells
(Tregs)
play
an
important
role
in
regulating
immune
homeostasis
health
and
disease.
Traditionally
their
suppressive
function
has
been
assayed
by
mixing
purified
cell
populations,
which
does
not
provide
accurate
picture
of
a
physiologically
relevant
response.
To
overcome
this
limitation,
we
here
developed
“single
suppression
profiling
human
Tregs”
(scSPOT).
scSPOT
is
more
informative
physiological
than
traditional
assays,
using
52-marker
CyTOF
panel,
division
detection
algorithm,
whole
PBMC
system
to
assess
the
Tregs
on
all
types
simultaneously.
By
employing
scSPOT,
observed
having
clearest
effects
CD8-EM
through
arrest,
cycle
inhibition,
effector
molecule
downregulation.
Additionally,
demonstrate
cellular
differentiation
tracking
drug
screening
applications
use
it
identify
potential
origin
Treg
phenotypic
split
critical
viral
infection
further
propose
modes
action
FDA-approved
drugs
Ipilimumab
Tazemetostat.
scalable,
robust,
widely
applicable,
may
be
used
better
understand
immunobiology
screen
for
therapeutic
compounds.
Viruses,
Journal Year:
2024,
Volume and Issue:
16(9), P. 1481 - 1481
Published: Sept. 18, 2024
The
inactivated
COVID-19
vaccine
has
demonstrated
high
efficacy
in
the
general
population
through
extensive
clinical
and
real-world
studies.
However,
its
effectiveness
immunocompromised
individuals,
particularly
those
living
with
HIV
(PLWH),
remains
limited.
In
this
study,
20
PLWH
15
HIV-seronegative
individuals
were
recruited
to
evaluate
immunogenicity
of
an
a
prospective
cohort
study.
median
age
was
42
years
31
years,
respectively.
Of
PLWH,
nine
had
been
on
ART
for
over
five
years.
anti-SARS-CoV-2
S-RBD
IgG
antibody
level
d
