The multifaceted role of macrophages in homeostatic and injured skeletal muscle DOI Creative Commons
Xingyu Wang, Lan Zhou

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: Oct. 25, 2023

Skeletal muscle is essential for body physical activity, energy metabolism, and temperature maintenance. It has excellent capabilities to maintain homeostasis regenerate after injury, which indispensably relies on stem cells, satellite cells (MuSCs). The quiescence, activation, differentiation of MuSCs are tightly regulated in homeostatic regenerating muscles. Among the important regulators intramuscular macrophages, functionally heterogeneous with different subtypes present a spatiotemporal manner regulate balance MuSC statuses. During chronic injury aging, macrophages often undergo aberrant turn disrupts regenerative repair. Growing evidence suggests that activation mainly triggered by altered microenvironment. trained immunity affects myeloid progenitors during hematopoiesis may also contribute. Aged immune system contribute, part, aging-related sarcopenia compromised skeletal As actively involved progression many diseases, manipulating their functional become promising therapeutic approach, requires comprehensive knowledge cellular molecular mechanisms underlying diverse activation. To this end, we discuss here current multifaceted role homeostasis,

Language: Английский

Immunology of human fibrosis DOI
Mallar Bhattacharya, Prakash Ramachandran

Nature Immunology, Journal Year: 2023, Volume and Issue: 24(9), P. 1423 - 1433

Published: July 20, 2023

Language: Английский

Citations

66
Repair of the Infarcted Heart: Cellular Effectors, Molecular Mechanisms and Therapeutic Opportunities DOI
Ingo Hilgendorf, Stefan Frantz, Nikolaos G. Frangogiannis

et al.

Circulation Research, Journal Year: 2024, Volume and Issue: 134(12), P. 1718 - 1751

Published: June 6, 2024

The adult mammalian heart has limited endogenous regenerative capacity and heals through the activation of inflammatory fibrogenic cascades that ultimately result in formation a scar. After infarction, massive cardiomyocyte death releases broad range damage-associated molecular patterns initiate both myocardial systemic responses. TLRs (toll-like receptors) NLRs (NOD-like recognize (DAMPs) transduce downstream proinflammatory signals, leading to upregulation cytokines (such as interleukin-1, TNF-α [tumor necrosis factor-α], interleukin-6) chemokines CCL2 [CC chemokine ligand 2]) recruitment neutrophils, monocytes, lymphocytes. Expansion diversification cardiac macrophages infarcted play major role clearance infarct from dead cells subsequent stimulation reparative pathways. Efferocytosis triggers induction release anti-inflammatory mediators restrain reaction set stage for fibroblasts vascular cells. Growth factor–mediated pathways, neurohumoral cascades, matricellular proteins deposited provisional matrix stimulate fibroblast proliferation myofibroblast conversion. Deposition well-organized collagen-based extracellular network protects catastrophic rupture attenuates ventricular dilation. Scar maturation requires signals inhibit activity prevent excessive fibrosis. Moreover, mature scar, neovessels acquire mural cell coat contributes stabilization microvascular network. Excessive, prolonged, or dysregulated accentuate adverse remodeling dysfunction. leukocytes can contribute arrhythmogenesis. Inflammatory pathways may be promising therapeutic targets attenuate failure progression arrhythmia generation patients surviving infarction.

Language: Английский

Citations

26
LIANA+ provides an all-in-one framework for cell–cell communication inference DOI Creative Commons
Daniel Dimitrov, Philipp Schäfer, Elias Farr

et al.

Nature Cell Biology, Journal Year: 2024, Volume and Issue: 26(9), P. 1613 - 1622

Published: Sept. 1, 2024

The growing availability of single-cell and spatially resolved transcriptomics has led to the development many approaches infer cell-cell communication, each capturing only a partial view complex landscape intercellular signalling. Here we present LIANA+, scalable framework built around rich knowledge base decode coordinated inter- intracellular signalling events from single- multi-condition datasets in both data. By extending unifying established methodologies, LIANA+ provides comprehensive set synergistic components study communication via diverse molecular mediators, including those measured multi-omics is accessible at https://github.com/saezlab/liana-py with extensive vignettes ( https://liana-py.readthedocs.io/ ) an all-in-one solution inference.

Language: Английский

Citations

25
Novel tumor-associated macrophage populations and subpopulations by single cell RNA sequencing DOI Creative Commons
Juanjuan Wang,

Ningning Zhu,

Lei Su

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 14

Published: Jan. 29, 2024

Tumor-associated macrophages (TAMs) are present in almost all solid tumor tissues. 16They play critical roles immune regulation, angiogenesis, stem cell activation, invasion and metastasis, resistance to therapy. However, it is unclear how TAMs perform these functions. With the application of single-cell RNA sequencing (scRNA-seq), has become possible identify TAM subpopulations associated with distinct In this review, we discuss four novel tumors based on core gene signatures by scRNA-seq, including FCN1 + , SPP1 C1Q CCL18 TAMs. Functional enrichment expression scRNA-seq data from different tissues found that may induce inflammation; potentially involved cancer whereas participate regulation suppression; And cells terminal immunosuppressive not only have a stronger function but also enhance metastasis. can be further divided into populations Meanwhile, will emerging evidence highlighting separating macrophage there exist potential disconnects between types identified their actual function.

Language: Английский

Citations

23
Single-cell RNA-seq analysis decodes the kidney microenvironment induced by polystyrene microplastics in mice receiving a high-fat diet DOI Creative Commons
Wenhao Xu, Shiqi Ye, Wangrui Liu

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Jan. 3, 2024

Abstract In recent years, the environmental health issue of microplastics has aroused an increasingly significant concern. Some studies suggested that exposure to polystyrene (PS-MPs) may lead renal inflammation and oxidative stress in animals. However, little is known about essential effects PS-MPs with high-fat diet (HFD) on development microenvironment. this study, we provided single-cell transcriptomic landscape kidney microenvironment induced by HFD mouse models unbiased RNA sequencing (scRNA-seq). The injury cell atlases mice were evaluated after continued exposure, or treated for 35 days. Results showed plus treatment aggravated profibrotic microenvironment, reshaping cellular components. First, found acted extracellular matrix organization epithelial cells, specifically proximal distal convoluted tubule inhibit induce ROS-driven carcinogenesis. Second, activated PI3K-Akt, MAPK, IL-17 signaling pathways endothelial cells. Besides, markedly increased proportions CD8 + effector T cells proliferating Notably, mononuclear phagocytes exhibited substantial remodeling enriched phosphorylation chemical carcinogenesis treatment, typified alterations tissue-resident M2-like PF4 macrophages. Multispectral immunofluorescence immunohistochemistry identified macrophages clear carcinoma (ccRCC) adjacent normal tissues, indicating activate might regulate pro-tumorigenic injury. conclusion, study first systematically revealed molecular variation immune scRNA-seq approach, which a basis decoding genitourinary understanding their potential mammals. Graphical

Language: Английский

Citations

17
Spatially restricted and ontogenically distinct hepatic macrophages are required for tissue repair DOI Creative Commons
Federico F. De Ponti, Anna Bujko, Zhuangzhuang Liu

et al.

Immunity, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Our understanding of the functional heterogeneity resident versus recruited macrophages in diseased liver is limited. A population lipid-associated (LAMs) has been reported to populate alongside Kupffer cells (KCs). However, precise roles these distinct macrophage subsets remain elusive. Here, using proteogenomics, we have identified LAMs multiple models injury. Moreover, found that this phenotype not specific macrophages, as a subset KCs can also adopt LAM-like mouse and human liver. By combining genetic targeting populations, determined both play crucial tissue repair. Specifically, triggering receptor expressed on myeloid 2 (TREM2) expression either or required for efficient clearance dying cells, enhancing repair preventing exacerbated fibrosis.

Language: Английский

Citations

4
Mechanisms of kidney fibrosis and routes towards therapy DOI
N. Yamashita, Rafael Kramann

Trends in Endocrinology and Metabolism, Journal Year: 2023, Volume and Issue: 35(1), P. 31 - 48

Published: Sept. 28, 2023

Language: Английский

Citations

32
Systems level identification of a matrisome-associated macrophage polarisation state in multi-organ fibrosis DOI Creative Commons
John F. Ouyang, Kunal Mishra, Yi Xie

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Sept. 14, 2023

Tissue fibrosis affects multiple organs and involves a master-regulatory role of macrophages which respond to an initial inflammatory insult common in all forms fibrosis. The recently unravelled multi-organ heterogeneity healthy fibrotic human disease suggests that expressing osteopontin (SPP1) associate with lung liver However, the conservation this SPP1+ macrophage population across different tissues its specificity diseases etiologies remain unclear. Integrating 15 single-cell RNA-sequencing datasets profile 235,930 tissue from heart, lung, liver, kidney, skin, endometrium, we extended association these tissues. We also identified subpopulation matrisome-associated genes (e.g., matrix metalloproteinases their inhibitors), functionally enriched for ECM remodelling cell metabolism, representative (MAM) polarisation state within macrophages. Importantly, MAM follows differentiation trajectory is associated core set regulon activity. without (SPP1+MAM-) show positive ageing mice humans. These results suggest advanced conserved resulting prolonged cues each microenvironment.

Language: Английский

Citations

26
LIANA+: an all-in-one cell-cell communication framework DOI Creative Commons
Daniel Dimitrov, Philipp Schäfer, Elias Farr

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 21, 2023

Abstract The growing availability of single-cell and spatially-resolved transcriptomics has led to the rapidly popularity methods infer cell-cell communication. Many approaches have emerged, each capturing only a partial view complex landscape Here, we present LIANA+, scalable framework decode coordinated inter- intracellular signalling events from single- multi-condition datasets in both data. Beyond integrating extending established methodologies rich knowledge base, LIANA+ enables novel analyses using diverse molecular mediators, including those measured multi-omics Accessible as an open-source Python package at https://github.com/saezlab/liana-py , provides comprehensive set synergistic components study Figure

Language: Английский

Citations

24
Immune mechanisms in fibrotic interstitial lung disease DOI
Mari Kamiya, Hannah M. Carter, Milena S. Espíndola

et al.

Cell, Journal Year: 2024, Volume and Issue: 187(14), P. 3506 - 3530

Published: July 1, 2024

Language: Английский

Citations

13