
Current Opinion in Neurobiology, Journal Year: 2024, Volume and Issue: 90, P. 102948 - 102948
Published: Dec. 21, 2024
Language: Английский
Current Opinion in Neurobiology, Journal Year: 2024, Volume and Issue: 90, P. 102948 - 102948
Published: Dec. 21, 2024
Language: Английский
Frontiers in Cellular Neuroscience, Journal Year: 2023, Volume and Issue: 17
Published: Nov. 16, 2023
Animal behavior, from simple to complex, is dependent on the faithful wiring of neurons into functional neural circuits. Neural circuits undergo dramatic experience-dependent remodeling during brief developmental windows called critical periods. Environmental experience periods plasticity produces sustained changes circuit function and behavior. Precocious period closure linked autism spectrum disorders, whereas extended synaptic thought underlie dysfunction in schizophrenia. Thus, resolving mechanisms that instruct timing important our understanding neurodevelopmental disorders. Control modulated by neuron-intrinsic cues, yet recent data suggest some determinants are derived neighboring glial cells (astrocytes, microglia, oligodendrocytes). As glia make up 50% human brain, how these diverse communicate with each other sculpt plasticity, especially specialized periods, essential fundamental development maintenance.
Language: Английский
Citations
15Trends in Neurosciences, Journal Year: 2024, Volume and Issue: 47(10), P. 803 - 818
Published: Sept. 24, 2024
Language: Английский
Citations
4Development, Journal Year: 2024, Volume and Issue: 151(15)
Published: July 15, 2024
Many genes are known to regulate retinal regeneration after widespread tissue damage. Conversely, controlling limited cell loss, as per degenerative diseases, undefined. As stem/progenitor responses scale injury levels, understanding how the extent and specificity of loss impact regenerative processes is important. Here, transgenic zebrafish enabling selective ganglion (RGC) ablation were used identify that RGC regeneration. A single multiomics-informed screen 100 identified seven knockouts inhibited 11 promoted Surprisingly, 35 out 36 and/or implicated being required for damage not The even enhanced kinetics, including proneural factors neurog1, olig2 ascl1a. Mechanistic analyses revealed ascl1a disruption increased propensity progenitor cells produce RGCs, i.e. 'fate bias'. These data demonstrate plasticity in mechanism through which Müller glia convert a stem-like state context function during Increased disease-relevant types specifically controlled will support development disease-tailored therapeutics.
Language: Английский
Citations
3PLoS Genetics, Journal Year: 2024, Volume and Issue: 20(4), P. e1011139 - e1011139
Published: April 26, 2024
As essential components of gene expression networks, transcription factors regulate neural circuit assembly. The homeobox factor encoding gene, gs 1 (gsx1), is expressed in the developing visual system; however, no studies have examined its role system formation. In zebrafish, retinal ganglion cell (RGC) axons that transmit information to brain terminate ten arborization fields (AFs) optic tectum (TeO), pretectum (Pr), and thalamus. Pretectal AFs (AF1-AF9) mediate distinct behaviors, yet we understand less about their development compared AF10 TeO. Using gsx1 zebrafish mutants, immunohistochemistry, transgenic lines, observed required for vesicular glutamate transporter, Tg(slc17a6b:DsRed), Pr, but not overall neuron number. mutants normal eye morphology, they exhibit impaired ability during prey capture. RGC axon volume mutant Pr TeO reduced, AF7 active feeding missing which consistent with reduced hunting performance. Timed laser ablation Tg(slc17a6b:DsRed)-positive cells reveals are necessary This work first implicate establishing identity functional circuits system.
Language: Английский
Citations
1Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)
Published: July 17, 2024
Abstract Visual systems have evolved to discriminate between different wavelengths of light. The ability perceive color, or specific light wavelengths, is important as color conveys crucial information about both biotic and abiotic features in the environment. Indeed, can drive distinct patterns activity vertebrate brain, yet what remains incompletely understood whether invoke etiologically relevant behavioral changes. To address how visible spectrum modulate performance, we use larval zebrafish a stereotypic light-search behavior. Prior work has shown that cessation triggers transitional behavior, which interrogate wavelength-dependent modulation. Using 8 narrow sources range, demonstrate all induce motor parameters consistent with search magnitude behavior sensitive underlying are modulated across short, medium, long wavelengths. However, our data also establishes not impacted by wavelength. define wavelength modulates performed additional assays alternative dual variable intensity. Last, tested blind larvae resolve components dependent changes potentially include signaling from non-retinal photoreception. These findings implications organisms be exposed varying laboratory natural settings therefore impose unique outputs.
Language: Английский
Citations
1STAR Protocols, Journal Year: 2023, Volume and Issue: 4(4), P. 102636 - 102636
Published: Oct. 13, 2023
Sensory experience instructs neurodevelopment and refines sensory processing. Here, we describe a minimally invasive protocol to immobilize zebrafish during early development control visual experience. We how prepare larvae for embedding in agarose at two separate timepoints development. Then build behavior rig use software track behaviors. Finally, detail analyzing behavioral data validate the determine outcomes of dependent plasticity. For complete details on execution this protocol, please refer Hageter et al. (2023).1
Language: Английский
Citations
2bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 16, 2024
Abstract As developing neural circuits become functional, they undergo a phase of heightened plasticity in response to intrinsic and/or extrinsic stimuli. These developmental windows are termed critical periods (CPs), because perturbations during the CP can lead lasting and significant change subsequent development, such as sub-optimal unstable networks. By contrast, same manipulations before or after does not create changes. Here, we have used Drosophila larval locomotor network study how different identified, connected elements respond perturbation, from pre-motor interneuron motoneuron, neuromuscular junction. Using heat stress an ecologically relevant stimulus, show that increasing temperature causes increased activity that, when applied CP, leads larvae crawl more slowly require longer recover electroshock-induced seizures, indicative decreased stability. Within central nervous system, find perturbation interneurons delivering synaptic drive motoneurons, which turn display reduced excitability. The peripheral junction, on other hand, maintains normal transmission, despite structural changes terminal overgrowth altered postsynaptic receptor field composition. Overall, our data demonstrate within differentially perturbation. Our results suggest underlying sequence, hierarchy, adjustment CPs, present highly tractable experimental model system with biology.
Language: Английский
Citations
0Neuromethods, Journal Year: 2024, Volume and Issue: unknown, P. 665 - 703
Published: Dec. 11, 2024
Language: Английский
Citations
0Current Opinion in Neurobiology, Journal Year: 2024, Volume and Issue: 90, P. 102948 - 102948
Published: Dec. 21, 2024
Language: Английский
Citations
0