bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Sept. 6, 2023
Abstract
Here
we
present
a
novel
fluorescence
microscopy
concept
which
enables
direct
integration
of
Super-Resolution
Microscopy
(SRM)
approaches
(SIM/Nanosizing,
STED,
SMLM,
MINFLUX,
SIMFLUX)
into
systems
with
working
distances
(WD)
up
to
the
multicentimeter
range
while
still
allowing
nanometer
scale
resolution
at
selected
sites.
This
becomes
possible
by
“synthetic
aperture”
illumination
mode
multiple,
constructively
interfering
excitation
beams
positioned
in
“Ring-Array”
arrangement
around
beam
free
interior
zone
containing
instrumentation
involved
complementary
imaging
modes.
The
feasibility
such
correlative
method
is
validated
extensive
numerical
simulations;
on
basis
these
calculations,
experimental
implementation
options
are
discussed.
Such
“Ring
Array”
modes
may
be
useful
for
various
methods,
as
combination
light
and
electron
same
device
(dCLEM);
or
low
NA/large
field-of-view
widefield
super-resolution
sites
(direct
Correlative
Opical
Microscopy/dCOLM).
Ring-Array
supported
will
open
perspectives
variety
disciplines,
from
material
sciences
biomedical
applications.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: March 22, 2024
Abstract
Genome
replication
requires
duplication
of
the
complete
set
DNA
sequences
together
with
nucleosomes
and
epigenetic
signatures.
Notwithstanding
profound
knowledge
on
mechanistic
details
replication,
major
problems
genome
have
remained
unresolved.
In
this
perspective
article,
we
consider
accessibility
machines
to
all
in
due
course,
maintenance
functionally
important
positional
structural
features
chromatid
domains
during
rapid
transition
CTs
into
prophase
chromosomes
two
chromatids.
We
illustrate
problem
EdU
pulse-labeling
(10
min)
chase
experiments
(80
performed
mouse
myeloblast
cells.
Following
light
optical
serial
sectioning
nuclei
3D
structured
illumination
microscopy
(SIM),
seven
intensity
classes
were
distinguished
as
proxies
for
increasing
compaction.
cells
fixed
immediately
after
pulse-label,
observed
a
relative
under-representation
EdU-labeled
low
density
classes,
representing
active
nuclear
compartment
(ANC),
an
over-representation
high
inactive
(INC).
Cells
revealed
even
more
pronounced
shift
classes.
This
finding
contrasts
previous
studies
transcriptional
topography
demonstrating
signatures
chromatin
RNAPII
their
discuss
these
findings
current
models
viewing
CDs
either
frameworks
or
phase-separated
droplets,
well
methodological
limitations
that
currently
prevent
integration
contrasting
evidence
spatial
transcription
common
framework
dynamic
architecture.
Biophysica,
Journal Year:
2024,
Volume and Issue:
4(2), P. 142 - 157
Published: March 29, 2024
For
many
biomedical
applications,
laser-assisted
methods
are
essential
to
enhance
the
three-dimensional
(3D)
resolution
of
a
light
microscope.
In
this
report,
we
review
possibilities
improve
3D
imaging
potential
by
axial
tomography.
This
method
allows
us
rotate
object
in
microscope
into
best
perspective
required
for
imaging.
Furthermore,
images
recorded
under
variable
angles
can
be
combined
one
image
with
isotropic
resolution.
After
brief
technical
state
art,
show
some
and
discuss
future
perspectives
Deep
View
Microscopy
Molecular
Imaging.
Physical review. E,
Journal Year:
2024,
Volume and Issue:
109(5)
Published: May 6, 2024
Using
scaling
arguments
to
model
peripheral
chromatin
localized
near
the
inner
surface
of
nuclear
envelope
(NE)
as
a
flexible
polymer
chain,
we
discuss
structural
properties
composed
alternating
lamin-associated
domains
(LADs)
and
inter-LADs.
Modeling
attraction
LADs
NE
by
de
Gennes'
self-similar
carpet,
which
treats
layer
fractal,
explains
two
major
experimental
observations.
(i)
The
high
density
close
periphery
decays
constant
distance
increases.
(ii)
Due
decreasing
mesh
size
towards
periphery,
carpet
inside
excludes
molecules
(via
nonspecific
interactions)
above
threshold
that
depends
on
from
periphery.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Sept. 6, 2023
Abstract
Here
we
present
a
novel
fluorescence
microscopy
concept
which
enables
direct
integration
of
Super-Resolution
Microscopy
(SRM)
approaches
(SIM/Nanosizing,
STED,
SMLM,
MINFLUX,
SIMFLUX)
into
systems
with
working
distances
(WD)
up
to
the
multicentimeter
range
while
still
allowing
nanometer
scale
resolution
at
selected
sites.
This
becomes
possible
by
“synthetic
aperture”
illumination
mode
multiple,
constructively
interfering
excitation
beams
positioned
in
“Ring-Array”
arrangement
around
beam
free
interior
zone
containing
instrumentation
involved
complementary
imaging
modes.
The
feasibility
such
correlative
method
is
validated
extensive
numerical
simulations;
on
basis
these
calculations,
experimental
implementation
options
are
discussed.
Such
“Ring
Array”
modes
may
be
useful
for
various
methods,
as
combination
light
and
electron
same
device
(dCLEM);
or
low
NA/large
field-of-view
widefield
super-resolution
sites
(direct
Correlative
Opical
Microscopy/dCOLM).
Ring-Array
supported
will
open
perspectives
variety
disciplines,
from
material
sciences
biomedical
applications.
