Effectiveness of newly isolated bacteriophages targeting multidrug-resistant Extraintestinal Pathogenic Escherichia coli strain (TZ1_3) in food preservation and mice health modulation

Food Chemistry, Journal Year: 2025, Volume and Issue: 472, P. 142833 - 142833

Published: Jan. 14, 2025

Language: Английский

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Multifluid Metabolomics Identifies Novel Biomarkers for Irritable Bowel Syndrome

Metabolites, Journal Year: 2025, Volume and Issue: 15(2), P. 121 - 121

Published: Feb. 12, 2025

Background/Objectives: Irritable bowel syndrome (IBS) is a complex disorder affecting 10% of the global population, but underlying mechanisms remain poorly understood. By integrating multifluid metabolomics, we aimed to identify metabolite markers IBS in large population-based cohort. Methods: We included individuals from TwinsUK with and without IBS, ascertained using Rome III criteria, analysed serum (232 cases, 1707 controls), urine (185 1341 stool (186 1284 controls) metabolites (Metabolon Inc.). Results: After adjusting for covariates, multiple testing, 44 unique (25 novel) were associated including lipids, amino acids, xenobiotics. Androsterone sulphate, sulfated steroid hormone precursor, was lower odds both (0.69 [95% confidence interval = 0.56-0.85], p 2.34 × 10-4) (0.75 [0.63-0.90], 1.54 10-3. Moreover, suberate (C8-DC) higher (1.36 [1.15-1.61]; 1.84 (0.76 [0.63-0.91]; 2.30 10-3). On contrary, 32 appeared be fluid-specific, indole, 13-HODE + 9-HODE, pterin, bilirubin (E,Z or Z,Z), urolithin. The remaining 10 one fluid suggestive evidence (p < 0.05) another fluid. Finally, identified androgenic signalling, dicarboxylates, haemoglobin, porphyrin metabolism significantly over-represented compared controls. Conclusions: Our results highlight utility multi-fluid approach research, revealing distinct metabolic signatures across biofluids.

Language: Английский

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Does Phage Therapy Need a Pan-Phage?

Pathogens, Journal Year: 2024, Volume and Issue: 13(6), P. 522 - 522

Published: June 20, 2024

The emergence of multidrug-resistant bacteria is undoubtedly one the most serious global health threats. One response to this threat that has been gaining momentum over past decade 'phage therapy'. According this, lytic bacteriophages are used for treatment bacterial infections, either alone or in combination with antimicrobial agents. However, ensure efficacy and broad applicability phage therapy, several challenges must be overcome. These encompass development methods strategies host range manipulation bypass resistance mechanisms developed by pathogenic bacteria, as case since advent antibiotics. As our knowledge understanding interactions between phages their hosts evolves, key issue define each application. In article, we discuss factors affect how determines classification into different categories action. For group, recent representative examples provided, together suggestions on groups can combat certain types infections. available methodologies expansion, through sequential adaptation a new pathogen genetic engineering techniques, also reviewed.

Language: Английский

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Phage therapy could be key to conquering persistent bacterial lung infections in children

npj Antimicrobials and Resistance, Journal Year: 2024, Volume and Issue: 2(1)

Published: Oct. 10, 2024

Persistent bacterial lung infections in children lead to significant morbidity and mortality due antibiotic resistance. In this paper, we describe how phage therapy has shown remarkable efficacy preclinical clinical studies, demonstrating therapeutic benefits through various administration routes. Ongoing trials are evaluating its safety effectiveness against different pathogens. Advancing systematic studies international collaboration could provide a viable alternative traditional antibiotics for persistent infections.

Language: Английский

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Perspective: on the future of fecal microbiota transplantation

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 9, 2024

Fecal Microbiota Transplantation (FMT) has shown to possess impressive potential benefit for a wide range of clinical indications. Due its inherent safety issues and efficacy constraints, the use personalized FMT analogs could be promising avenue. The development such will require detailed understanding their functionality, encompassing not only microbe-host interactions microbial taxa that are involved, but also ecological dimensions an overview gastrointestinal sites where these relevant take place. Moreover, characterization have been lost due diminished exposure beneficial microbes, as consequence Western lifestyle, may lead creation future with capacity restore functionalities we lost.

Language: Английский

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Human and gut microbiota synergy in a metabolically active superorganism: a cardiovascular perspective

Frontiers in Cardiovascular Medicine, Journal Year: 2024, Volume and Issue: 11

Published: Oct. 11, 2024

Despite significant advances in diagnosis and treatment over recent decades, cardiovascular disease (CVD) remains one of the leading causes morbidity mortality Western countries. This persistent burden is partly due to incomplete understanding fundamental pathogenic mechanisms, which limits effectiveness current therapeutic interventions. In this context, evidence highlights pivotal role immuno-inflammatory activation by gut microbiome influencing disorders, potentially opening new avenues. Indeed, while atherosclerosis has been established as a chronic inflammatory arterial wall, accumulating data suggest that immune system regulation anti-inflammatory pathways mediated microbiota metabolites play crucial range CVDs, including heart failure, pericardial disease, arrhythmias, cardiomyopathies. Of particular interest emerging how tryptophan metabolism-by both host microbiota-converges on Aryl hydrocarbon Receptor (AhR), key regulator homeostasis. review seeks enhance our inflammation CVD, with focus microbiome-derived metabolites, such indoles their derivatives, contribute cardioimmunopathology. By exploring these we aim facilitate development novel, microbiome-centered strategies for combating CVD.

Language: Английский

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Association between diet quality and all-cause mortality in a large Dutch cohort

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(8), P. e0302905 - e0302905

Published: Aug. 23, 2024

Food-based dietary guidelines are helpful for governments and health agencies to encourage healthy eating at the population level. In order assess adherence such guidelines, index scores have been developed, version in Netherlands being Dutch Healthy Diet-index (2015) (DHD2015-index), which reflect 2015 guidelines. Because a higher diet quality, i.e. is associated with better outcomes, DHD2015-index score would also mean outcomes on measures of health, as all-cause mortality. The present study aimed elucidate this by investigating association between mortality using data from 97 999 participants Lifelines cohort study. For analyses, Cox Proportional Hazards regression was used, whilst accounting age, sex, physiological measurements, exercise, biochemical lifestyle variables. There strong negative Hazard ratios below 60 were approximately 1.2x larger than mean. Every 10 unit increase 90 led 0.1 reduction hazard ratio, every highest 0.05. ratio lowest quartile 1.14 (95% CI = 1.04–1.26), whereas that 0.88 0.84–0.92). Our results show clear inverse relationship

Language: Английский

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Modulating intestinal viruses: A potential avenue for improving metabolic diseases with unresolved challenges

Life Sciences, Journal Year: 2024, Volume and Issue: 361, P. 123309 - 123309

Published: Dec. 12, 2024

Language: Английский

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