Frontiers in Neural Circuits,
Journal Year:
2023,
Volume and Issue:
17
Published: Aug. 30, 2023
Despite
the
importance
of
postsynaptic
inhibitory
circuitry
targeted
by
mid/long-range
projections
(e.g.,
top-down
projections)
in
cognitive
functions,
its
anatomical
properties,
such
as
laminar
profile
and
neuron
type,
are
poorly
understood
owing
to
lack
efficient
tracing
methods.
To
this
end,
we
developed
a
method
that
combines
conventional
adeno-associated
virus
(AAV)-mediated
transsynaptic
with
distal-less
homeobox
(Dlx)
enhancer-restricted
expression
system
label
neurons.
We
called
"Dlx
Interneuron-SpECific
Tracing"
(DISECT).
applied
DISECT
corticocortical
circuit
from
secondary
motor
cortex
(M2)
primary
somatosensory
(S1)
wild-type
mice.
First,
injected
AAV1-Cre
into
M2,
which
enabled
Cre
recombinase
M2-input
recipient
S1
Second,
AAV1-hDlx-flex-green
fluorescent
protein
(GFP)
transduce
GFP
neurons
Cre-dependent
manner.
succeeded
exclusively
labeling
S1.
Laminar
analysis
labeled
via
indicated
were
distributed
superficial
deep
layers
This
distribution
was
aligned
density
axons
projecting
M2.
further
classified
types
using
immunohistochemistry
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 6, 2024
ABSTRACT
Neurovascular
coupling,
linking
neuronal
activity
to
cerebral
blood
flow,
is
altered
early
in
neurological
disorders
and
underlies
functional
brain
imaging.
This
process
involves
numerous
cellular
players.
Among
them
inhibitory
interneurons
receive
increasing
attention,
but
how
they
control
flow
remains
elusive.
study
elucidates
the
mechanisms
by
which
somatostatin
bidirectionally
neurovascular
coupling.
Patch
clamp
recordings
in,
ex
vivo,
cortical
slices
from
mice
expressing
channelrhodopsin-2
interneurons,
revealed
that
these
neurons
are
supralinearly
activated
at
low-frequencies
(<
5
Hz)
efficiently
photostimulated
frequencies
up
20
Hz.
Ex
vivo
vascular
imaging
showed
low-frequency
(2
photostimulation
triggered
vasodilation
whereas
high-frequency
(20
induced
vasoconstriction.
Histochemistry
subpopulations
of
expressed
nitric
oxide
synthase,
and/or
neuropeptide
Y
a
greater
extent.
Consistently,
pharmacological
investigations
release
activation
soluble
guanylate
cyclase.
In
contrast,
vasoconstriction
Y1
receptor.
These
findings
provide
valuable
insights
into
coupling
help
understand
mechanism
underlying
signals.
Frontiers in Neural Circuits,
Journal Year:
2023,
Volume and Issue:
17
Published: Aug. 30, 2023
Despite
the
importance
of
postsynaptic
inhibitory
circuitry
targeted
by
mid/long-range
projections
(e.g.,
top-down
projections)
in
cognitive
functions,
its
anatomical
properties,
such
as
laminar
profile
and
neuron
type,
are
poorly
understood
owing
to
lack
efficient
tracing
methods.
To
this
end,
we
developed
a
method
that
combines
conventional
adeno-associated
virus
(AAV)-mediated
transsynaptic
with
distal-less
homeobox
(Dlx)
enhancer-restricted
expression
system
label
neurons.
We
called
"Dlx
Interneuron-SpECific
Tracing"
(DISECT).
applied
DISECT
corticocortical
circuit
from
secondary
motor
cortex
(M2)
primary
somatosensory
(S1)
wild-type
mice.
First,
injected
AAV1-Cre
into
M2,
which
enabled
Cre
recombinase
M2-input
recipient
S1
Second,
AAV1-hDlx-flex-green
fluorescent
protein
(GFP)
transduce
GFP
neurons
Cre-dependent
manner.
succeeded
exclusively
labeling
S1.
Laminar
analysis
labeled
via
indicated
were
distributed
superficial
deep
layers
This
distribution
was
aligned
density
axons
projecting
M2.
further
classified
types
using
immunohistochemistry
