Deep longitudinal lower respiratory tract microbiome profiling reveals genome-resolved functional and evolutionary dynamics in critical illness

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Sept. 27, 2024

Language: Английский

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Isolation and Characterization of Lactobacillus gasseri Strains from Women for Potential Vaginal Health Applications

Microbiology Research, Journal Year: 2025, Volume and Issue: 16(1), P. 12 - 12

Published: Jan. 10, 2025

Lactobacillus, a genus of lactic acid bacteria, is known to coexist symbiotically in the female vaginal microbiota and has gained attention as potential probiotic with benefits for reproductive health. This study aimed evaluate Lactobacillus gasseri BELG74(BELG74), isolated from Korean women, promoting health through growth ability, pH reduction, production, antimicrobial activity. Among 36 strains, BELG74 demonstrated highest capacity at 1.84 × 109 CFU/mL lowest 3.84. produced most concentration 20.12 g/L, which correlated anti-pathogenic activity against Gardnerella vaginalis, Fannyhessea vaginae, Candida albicans more than 90%. It also showed high resistance (92.2%) bile (25.3%), ensuring its survival gastrointestinal tract. Furthermore, exhibited strong biofilm formation adhesion 28.7% HeLa cells, making it effective colonizing environment suppressing pathogenic bacteria. The reduction IL-1β by 63% suggested anti-inflammatory effects. Additionally, effectively neutralized trimethylamine ammonia over 99.9%, suggesting ability reduce unpleasant odors. These findings indicate that could be promising improving health, further clinical studies needed confirm these benefits.

Language: Английский

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Exploring the Potential Use of Probiotics, Prebiotics, Synbiotics, and Postbiotics as Adjuvants for Modulating the Vaginal Microbiome: a Bibliometric Review

Probiotics and Antimicrobial Proteins, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 17, 2025

Language: Английский

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Changes in vaginal Ureaplasma and Lactobacillus due to antibiotic regimen for premature rupture of membranes

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(2), P. e0306958 - e0306958

Published: Feb. 18, 2025

Preterm premature rupture of membranes (PPROM) is associated with preterm delivery and neonatal complications. PPROM often complicated by intra-amniotic inflammation and/or microbial invasion the amniotic cavity Ureaplasma or Mycoplasma . Various prophylactic antibiotic therapies have been proposed to prolong latency between delivery, reduce risk clinical chorioamnionitis, improve However, information on potential azithromycin administration load vaginal remains lacking. This prospective cohort study included singleton pregnancies managed antibiotics for at less than 36 weeks gestation. All patients received standard regimen PPROM, which consisted a single oral intravenous ampicillin every 2 days followed 5 amoxicillin. Vaginal swabs samples were collected when was confirmed after administration. The main outcome measures investigate changes in Ureaplasma, Mycoplasma, Lactobacillus spp. due regimen. In addition, association presence , pregnancy outcomes, complications examined. Out 82 eligible 51 had positive Thirty-six (52.2%) completed Among those who regimen, 75% experienced an increase levels. For delivered before completing all doses, 40% increased Furthermore, resulted decreased almost cases. It suggested that may not be effective targeting Since this did search resistance genes, it cannot determined responsible present results. found sepsis bronchopulmonary dysplasia. Future studies are needed revalidate current therapy PPROM.

Language: Английский

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Refining Unfavorable Vaginal Microbial Community in Infertile Women Subjected to Precision Probiotic Intervention: An Exploratory Single-Arm, Prospective, Open-Label, Interventional Study

Microorganisms, Journal Year: 2025, Volume and Issue: 13(3), P. 547 - 547

Published: Feb. 28, 2025

Background and aims: Vaginal microbiomes have been classified into five different general categories, termed Community State Type (CSTs), with CST-III CST-IV often associated vaginal dysbiosis which makes women more prone to recurrent infections assisted reproductive technology (ART) failure. Since a healthy microbiome is one of the key steps for successful reproduction, we investigated impact modulating microbiota through oral administration probiotic formula consisting consortium vaginal-specific lactobacilli prebiotics (Personal Flora 2®). Methods: We recruited 32 who had previous failed IVF cycles were scheduled undergo ART. examined composition before after supplementation using 16S ribosomal RNA (rRNA) sequencing technology. Results: Our data show noticeable modulation upon supplementation. In particular, precision intervention lowers species diversity, favoring dominance Lactobacillus (p = 0.015) Bifidobacterium 0.000) whilst decreasing percentage Atopobium 0.003), Gardnerella 0.022), Prevotella 0.000). Conclusions: Although are generally considered detrimental, gynecologists should not refrain from performing in these if they previously subjected probiotics treatments, as specific reduces presence pathogenic bacteria promoting increase ecosystem, could pregnancy success.

Language: Английский

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Strain-level variation among vaginal Lactobacillus crispatus and Lactobacillus iners as identified by comparative metagenomics

npj Biofilms and Microbiomes, Journal Year: 2025, Volume and Issue: 11(1)

Published: March 23, 2025

The vaginal microbiome, a relatively simple, low diversity ecosystem crucial for female health, is often dominated by Lactobacillus spp. Detailed strain-level data, facilitated shotgun sequencing, can provide greater understanding of the mechanisms colonization and host-microbe interactions. We analysed 354 metagenomes from pregnant women in Ireland to investigate metagenomic community state types variation, focusing on cell surface interfaces. Our analysis revealed multiple subspecies, with crispatus iners being most dominant. found genes, including putative mucin-binding distinct L. subspecies. Using 337 metagenome-assembled genomes, we observed higher number strain-specific genes related wall biogenesis, carbohydrate amino acid metabolism, many under positive selection. A glycan gene cluster was predominantly but absent Gardnerella vaginalis. These findings highlight factors associated colonisation

Language: Английский

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Leveraging the microbiome to combat antibiotic resistant gynecological infections

npj Antimicrobials and Resistance, Journal Year: 2025, Volume and Issue: 3(1)

Published: April 23, 2025

Abstract The vaginal resistome can be considered a collection of the resistant determinants in microbiome. Here we review including microbes and genes harbored common gynecological infections, that participate horizontal gene transfer, host factors contribute to resistome, therapies. Finally, provide perspective on technologies leveraged study remaining challenges.

Language: Английский

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MASS cohort: Multicenter, longitudinal, and prospective study of the role of microbiome in severe pneumonia and host susceptibility

iMeta, Journal Year: 2024, Volume and Issue: 3(4)

Published: June 25, 2024

The MASS cohort comprises 2000 ICU patients with severe pneumonia, covering community-acquired hospital-acquired and ventilator-associated sourced from 19 hospitals across 10 cities in three provinces. A wide array of samples including bronchoalveolar lavage fluid, sputum, feces, whole blood are longitudinally collected throughout patients' stays. study seeks to uncover the dynamics lung gut microbiomes their associations pneumonia host susceptibility, integrating deep metagenomics transcriptomics detailed clinical data. Severe (CAP), (HAP), (VAP), remains a major challenge critical care, high morbidity mortality [1, 2]. disruption microbiome, as seen SARS-CoV-2 [3], is associated disease progression by triggering inflammatory responses influencing immune functions [4], notably through alveolar macrophages, which crucial for pathogen clearance [5]. interplay between microbiomes, known gut-lung axis, also plays role host's response overall health, especially intensive care unit (ICU) [6, 7]. Dysbiosis these may contribute conditions like acute respiratory distress syndrome (ARDS), impacting patient outcomes survival [6]. However, understanding connections microbiota susceptibility still limited, often hindered small sizes lack comprehensive data multiple centers [8, 9]. To address gaps, we introduce cohort—a multicenter, longitudinal, prospective microbiome susceptibility. From 2023 2025, plan collect fluid (BALF), alongside Our goal relationship (see "Outcome assessment susceptibility" Supporting Information), an extension our previous multi-center research [10, 11]. In addition, multicenter observational serve surveillance tool emerging infectious diseases, laying solid foundation subsequent validation studies interventions pneumonia. designed prospective, comprising 26 ICUs medical centers, spanning provinces, total more than 800 beds (Figure 1A Table S1). These possess extensive expertise diagnosis treatment equipped skilled staff. Among 14 situated seven Zhejiang province, four two Henan one Shaanxi province. We utilize province discovery (ZJ cohort), while other provinces external cohorts (non-ZJ cohorts). Alternatively, considering potential geographic variation allocate 20% each respective cohort. trial has received approval Ethics Committee First Affiliated Hospital University School Medicine (ethic ID: IIT20230371B IIT20230183B-R1) registered on ClinicalTrials.gov (NCT06114784). recruit longitudinal sample collection follow-up visits 1B S2). Upon enrollment, basic information, history, imaging documented "Collection data" Information). Simultaneously, BALF, stool, laboratory culture next-generation sequencing (NGS), among analyses "Methodologies biological processing" "Methods bioinformatics analysis" When applicable, will be at 4, 7, 14, 21 days after admission, ventilator parameters recorded those days. Drug usage Days 28, complications Day 28. Additionally, conducted 90 365 (Table primary aims are: (1) investigating dynamic characteristics (2) exploring secondary include: examining relationships treatments, drug usage, exposure; variations drug-resistance genes horizontal gene transfer (HGT) within patients; (3) identifying disparities CAP, HAP, VAP; (4) charting virome landscape; (5) predicting 28-day based (6) monitoring predict bacterial infection. timeline been established current progress, "Progress cohort" section 1C). commenced recruitment September At time writing announcement (February 2024), have successfully recruited 286 15 cohort"). aim enroll up delineate infections caused various pan-kingdom pathogens. Since definitive not possible suspected included "Definitions" Following Dr. Lingtong Huang, project leader, principal investigator center independently conduct diagnoses all patients. cases diagnostic disagreement, Hongliu Cai participate final evaluation. All enrolled fully informed provide signed consent, without receiving additional financial compensation. For unconscious patients, consent form his/her next kin. inclusion exclusion criteria outlined below: Inclusion criteria: Patients newly acquired that meet following invasive or noninvasive mechanical ventilation failure, positive end-expiratory pressure (PEEP) ≥ 5 cm; high-flow oxygen therapy fraction inspired (FiO2) 50% arterial partial (PaO2) FiO2 ratio <300. Exclusion expected length stay less 1 day; hospitalized 7 before being transferred; pregnant; under 18 years old. sputum endotracheal aspirate (ETA), stool rectal swab, blood, serum 1, when applicable Sample occur 24-h window around planned sampling time. example, if 4 impractical, rescheduled 3–5 (Day ± 24 h). minimize batch effect supplies, participating use standardized nucleic acid preservation solutions, tubes, saline solutions supplied quarterly Medicine. Given typical low microbial yield hospital establish negative environmental instrument controls. conclude discharged transferred ICU. protocols controls, along rest analytical can found Information. As February 2024, had criteria. Of these, 174 (60.84%) were ZJ 112 (39.16%) non-ZJ 2A). consisted 194 (67.83%) 22 stroke-associated 69 (24.13%) (5.24%) VAP 2B). (1.4%) trauma-associated injury, unlikely median age was (interquartile range [IQR]: 18), body mass index 22.86 (IQR: 4.96). predominantly male (209 73.08%), 32 (11.2%) reporting history alcohol 76 (26.57%) smoking 2C,D). terms extracorporeal organ support, 251 (87.76%) required (IMV), 55 (19.23%) continuous renal replacement (CRRT), 17 (5.94%) underwent ECMO 2D). 202 (70.6%) admitted same day admission. 84 (29.4%) average 7.7 hospitalization. 136 (47.5%) emergency department, (26.6%) internal medicine, 47 (16.4%) ICUs, 25 (8.7%) surgery 2E). Outcomes April 153 (53.5%) improvements, in-hospital deaths, 11 (3.8%) transfers higher-level hospitals, six (2.1%) remaining. certain cultural practices China, 36 (12.6%) voluntarily death, 48 (16.8%) patients—or families—opted discontinuing pursuing palliative care. (9.09%) extended stays beyond 28 By end amassed 502 484 386 282 292 2F). Concurrently, 30 control consumables collected. BALF specimens sent National Institute Pathogen Biology, CAMS & PUMC aliquoting, using phosphate-buffered during this process. This large-cohort, approach robustly characterize Chinese settings, advanced analytics explore microbiome's diversity its diseases. study's limitations include generalizability absence nonsevere group restricts comparative analyses. An ongoing healthy project, led Professor Chao Jiang, (supported Natural Science Foundation grant no. 82341109). nature prevents establishing direct causality outcomes. Future should diverse demographics [12], groups, experimental validation. Antibiotic varying comorbidity profiles present confounding factors, although propensity score matching mitigate effects. given limited how factors affect [13, 14], incorporate exposome into deepen [15-17]. conclusion, establishes rich resource influence metagenomic, transcriptomic, phenomic significantly enhance pathogens, risk poor prognosis, determinants Lili Ren, Jianwei Wang, Lingling Tang trials. Xin Wei, Li Guo, Cai, Silan Gu wrote plan. Guojun He, Longxian Lv, Mingqiang Hongyu Dan Dang, Shengfeng Peng Shen, Qianqian Yinghe Xu, Yongpo Xuwei Lin Zhong, Yonghui Xiong, Kankai Tang, Fengqi Liu, Yuxin Leng, Minghui Cheng, Yijiao Han, Xindie Baoyue Lin, Huanzhang Shao, Nan Ning Zhang, Xuntao Deng, Muhua Dai, Yujie Pan, Bin Yang participated discussion enrollment. authors read manuscript approved it publication. work supported China (grant 82202356, 82341109, 82173645), Provincial Fund LTGY24H190001), Technology Major Project 2017ZX10204401), Key Research Development Program 2022YFC2504501, 2022YFA1304300, 2021YFC2301805). declare no conflict interest. ethics committees (IIT20230371B hospitals. support findings available request corresponding author. publicly due privacy ethical restrictions. No new scripts generated study. Information: (methods, tables, scripts, graphical abstract, slides, videos, translated version, updated materials) online DOI iMeta http://www.imeta.science/. Please note: publisher responsible content functionality any supporting information authors. Any queries (other missing content) directed author article.

Language: Английский

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The costs and benefits of a dynamic host microbiome

Trends in Ecology & Evolution, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

All species host a rich community of microbes. This microbiome is dynamic, and displays seasonal, daily, even hourly changes, but also needs to be resilient fulfill important roles for the host. In evolutionary ecology, focus dynamism has been on how it can facilitate adaptation novel environments. However, an hitherto largely overlooked issue that keep its in check, which costly leads trade-offs with investing other fitness-related traits. Investigating these natural vertebrate systems by collecting longitudinal data will lead deeper insight into mechanisms shape host-microbiome interactions.

Language: Английский

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Molecular characterization of vaginal microbiota using a new 22-species qRT-PCR test to achieve a relative-abundance and species-based diagnosis of bacterial vaginosis

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: June 28, 2024

Background Numerous bacteria are involved in the etiology of bacterial vaginosis (BV). Yet, current tests only focus on a select few. We therefore designed new test targeting 22 BV-relevant species. Methods Using 946 stored vaginal samples, qPCR that quantitatively identifies species was designed. The distribution and relative abundance each species, α- β-diversities, correlation, co-existence were determined per sample. A diagnostic index modeled from data, trained, tested to classify samples into BV-positive, BV-negative, or transitional BV. Results identified all targeted with 95 – 100% sensitivity specificity within 8 hours (from sample reception). Across most Lactobacillus iners, crispatus, jensenii, Gardnerella vaginalis, Fannyhessea (Atopobium) vaginae, Prevotella bivia , Megasphaera sp. type 1 relatively abundant. BVAB-1 more abundant distributed than BVAB-2 BVAB-3. No Mycoplasma genitalium found. inter-sample similarity very low, correlations existed between key which used model, train, index: MDL-BV . using both markers, classified three microbiome states. Testing this our 491 318 137 Although important differences BV status observed different age groups, races, pregnancy status, they statistically insignificant. Conclusion diverse large number races including pregnant women, qRT-PCR efficiently diagnosed reception), BV-associated

Language: Английский

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