Protocol for multi-component reconstitution of stress granules in vitro DOI Creative Commons

Zhiying Yao,

Yi Liu, Peiguo Yang

et al.

STAR Protocols, Journal Year: 2025, Volume and Issue: 6(1), P. 103666 - 103666

Published: March 1, 2025

To date, stress granule studies mainly focus on cell models. The diversity of molecules in granules makes it challenging to uncover the function each molecule regulation. Here, we provide a protocol reconstitute with multi-components vitro. We describe steps for strain amplification, protein purification, and liquid-liquid phase separation (LLPS). multi-component reconstitution system constructed this also provides technique other condensate For complete details use execution protocol, please refer Yao et al.1.

Language: Английский

Protocol for multi-component reconstitution of stress granules in vitro DOI Creative Commons

Zhiying Yao,

Yi Liu, Peiguo Yang

et al.

STAR Protocols, Journal Year: 2025, Volume and Issue: 6(1), P. 103666 - 103666

Published: March 1, 2025

To date, stress granule studies mainly focus on cell models. The diversity of molecules in granules makes it challenging to uncover the function each molecule regulation. Here, we provide a protocol reconstitute with multi-components vitro. We describe steps for strain amplification, protein purification, and liquid-liquid phase separation (LLPS). multi-component reconstitution system constructed this also provides technique other condensate For complete details use execution protocol, please refer Yao et al.1.

Language: Английский

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