Effects of Exercise on Post-Stroke Depression: A Systematic Review and Meta-Analysis of Randomized Controlled Trials

Life, Journal Year: 2025, Volume and Issue: 15(2), P. 285 - 285

Published: Feb. 12, 2025

Numerous studies have investigated the effects of exercise on post-stroke depression (PSD), yet findings remain inconclusive. This study aims to evaluate impact depressive symptoms in stroke patients and identify most effective protocols for this population. A systematic review Embase, PubMed, Cochrane Library, Web Science, Scopus databases was conducted, with a search cutoff date 13 September 2024. Quantitative synthesis employed assess intervention effects, effect sizes expressed as standardized mean differences (SMDs) 95% confidence intervals efficacy alleviating PSD. total 24 met inclusion criteria. The results indicated that significantly alleviated (SMD = -0.18; p 0.007). Specifically, multicomponent training emerged reducing -0.24; 0.008). Additionally, programs duration ≥12 weeks (SMD, -0.17; 0.04), ≥3 sessions per week -0.20; 0.02), <60 min session -0.19; 0.05), <180 -0.27; 0.02) were found be Exercise represents an strategy managing PSD, potentially serving optimal intervention. These provide evidence clinicians, recommending engage at least three times weekly, individual not exceeding 60 min. By increasing frequency exercise, cumulative weekly time should ideally below 180 outcomes.

Language: Английский

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Protective effects of small RNAs encapsulated in Artemisia Capillaris-derived exosomes against non-alcoholic fatty liver disease

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 6, 2025

Artemisia capillaris, a traditional medicinal plant, is renowned for its therapeutic properties, including the promotion of anti-inflammatory and bile secretion. Notably, it has demonstrated efficacy in treatment jaundice. This study aimed to evaluate potential capillaris-derived exosomes (ACDEs) as novel approach non-alcoholic fatty liver disease (NAFLD). The physicochemical properties ACDEs were isolated characterized using differential centrifugation, was evaluated an vivo methionine-choline-deficient (MCD) diet induced NAFLD mouse model. In vitro, hepatocytes treated with palmitic acid (PA) simulate high fat environment. Intracellular triglycerides (TG) total cholesterol (TC) levels quantified, Oil Red O staining assessed. Additionally, expression proteins RNAs associated lipogenesis inflammation analyzed. model exhibited notable damage, lipid deposition inflammatory responses. However, broad pharmacological activities, effectively reversing hepatic accumulation damage. vitro experiments revealed that internalized by AML12 cells via macropinocytosis caveolin-mediated endocytosis. ameliorated dysregulated metabolism inhibited High throughput sequencing further identified distinct small RNA profile ACDEs, indicating involvement interspecies physiological regulation. conclusion, this provides evidence offers perspective development capillaris-based therapies NAFLD, related metabolic disorders, hepatitis.

Language: Английский

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Redefining bladder cancer treatment: innovations in overcoming drug resistance and immune evasion

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 22, 2025

Bladder cancer is one of the most common malignancies urinary system and has always presented great challenges in treatment due to its intricate biological features high recurrence rates. Although developments were achieved immunotherapy targeted therapies within last decade, therapeutic outcomes for a number patients remain unsatisfactory, particularly as long-term efficacy. Review discusses molecular mechanisms developed during process bladder progression: genetic epigenetic alterations, dynamics tumor microenvironment (TME), dysregulation abnormal activation various signaling pathways—all contributing resistance. It mutation, especially both low- high-grade tumors, that, alongside modifications, plays considerable role aggressiveness drug TME, comprising cancer-associated fibroblasts (CAFs), immunosuppressive cells, different components extracellular matrix (ECM), orchestrates setting that fosters growth immune evasion confers resistance on any regime might be used. The review also provides an overview PI3K/AKT MAPK pathways progression development against them. Further, it immunotherapy, including those involving checkpoint inhibitors. Other promising approaches include new strategies target not only but checkpoints combination therapies. This aims contribute elaboration more effective personalized by fully understanding underlying involved cancer.

Language: Английский

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Macrophage polarization-mediated PKM2/mTORC1/YME1L signaling pathway activation in fibrosis associated with Cardiorenal syndrome

Cellular Signalling, Journal Year: 2025, Volume and Issue: unknown, P. 111664 - 111664

Published: Feb. 1, 2025

Language: Английский

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Integrative analysis of single-cell and bulk RNA sequencing reveals the oncogenic role of ANXA5 in gastric cancer and its association with drug resistance

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: March 7, 2025

Background Gastric cancer (GC) remains a leading cause of cancer-related mortality, with over one million new cases and 769,000 deaths reported in 2020. Despite advancements chemotherapy, surgery, targeted therapies, delayed diagnosis due to overlooked early symptoms leads poor prognosis. Methods We integrated bulk RNA sequencing single-cell datasets from TCGA, GEO, OMIX001073, employing normalization, batch effect correction, dimensionality reduction methods identify key cell populations associated GC invasion epithelial-mesenchymal transition (EMT), as well analyze the tumor immune microenvironment. Results Our analysis identified MUC5AC+ malignant epithelial cluster significant player EMT. Cluster 1, representing this population, exhibited higher EMT scores compared other clusters. Survival showed that high abundance 0 correlated improved survival rates (P=0.012), whereas 1 was poorer outcomes (P=0.045). A prognostic model highlighted ANXA5 GABARAPL2 two critical genes upregulated tumors. High-risk patients demonstrated increased infiltration worse prognosic. Analysis mutation burden (TMB) indicated low TMB high-risk group had worst Wet-lab validation experiments confirmed oncogenic role ANXA5, showing its facilitation proliferation, invasion, migration while suppressing apoptosis. Conclusion This study offers novel insights into subpopulations cells their roles progression. It provides potential therapeutic targets combat GC, contributing crucial understanding fundamental mechanisms drug resistance gastrointestinal cancers.

Language: Английский

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Therapeutic strategies for adrenocortical carcinoma: integrating genomic insights, molecular targeting, and immunotherapy

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: March 12, 2025

Adrenocortical carcinoma (ACC) is an uncommon and highly aggressive cancer originating in the adrenal cortex, characterized by a high likelihood of recurrence unfavorable survival rates, particularly advanced disease stages. This review discusses complex molecular pathogenesis ACC, focusing on critical pathways implicated tumorigenesis providing potential targets for therapy: Wnt/β-catenin signaling pathway, IGF2/IGF1R axis, apoptosis pathway regulated p53. Current treatment strategies include surgical resection mitotane, sole adrenolytic agent approved FDA; however, its effects are suboptimal. Cytotoxic chemotherapy combined with mitotane may be applied, but benefits limited so far. In following review, we outline emerging targeted therapies, such as mTOR inhibitors tyrosine kinase (TKIs), which show favorable preclinical clinical data, especially treatment-resistant ACC. We also emphasize possible role immune checkpoint (ICIs) management although their effectiveness still under study. Upcoming trends involve forms personalized medicine, where profiling integrated to identify actionable biomarkers administered therapies. will attempt provide comprehensive framework how recent breakthroughs genomics coupled advances therapies immunotherapy, can improve management.

Language: Английский

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Exploration of SUSD3 in pan-cancer: studying its role, predictive analysis, and biological significance in various malignant tumors in humans

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: March 21, 2025

Background The SUSD3 protein, marked by the Sushi domain, plays a key role in cancer progression, with its expression linked to tumor advancement and patient prognosis. Altered levels could serve as predictive biomarker for progression. Recognized novel susceptibility marker, presents promising target antibody-based therapies, offering potential approach prevention, diagnosis, treatment of breast cancer. Methods Using HPA GeneMANIA platforms, distribution protein across tissues was analyzed, while healthy were compared using Cancer Genome Atlas data. TISCH STOmics DB databases facilitated mapping different cell types spatial relationship markers. Univariate Cox regression assessed prognostic significance various cancers. Genomic alterations explored through cBioPortal database. predictor immunotherapy response investigated TIMER2.0, GSEA/GSVA identified related biological pathways. Drugs targeting CellMiner, CTRP, GDSC databases, complemented molecular docking studies. In vitro experiments demonstrated that knockdown lines significantly reduced proliferation migration. Results variations pan-cancer cohorts are closely prognosis malignancies. microenvironment (TME), is predominantly expressed monocytes/macrophages CD4+ T cells. Research indicates strong correlation between biomarkers, immune infiltration, immunomodulatory factors. To explore regulatory role, StromalScore, ImmuneScore, ESTIMATE, Immune Infiltration metrics employed. Molecular studies revealed selumetinib inhibits proliferation. Finally, Conclusion These findings provide valuable insights establish foundation further exploration SUSD3’s pan-carcinomas. Additionally, they offer perspectives targets development innovative therapeutic strategies treatment.

Language: Английский

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Adipose-derived small extracellular vesicle miR-146a-5p targets Fbx32 to regulate mitochondrial autophagy and delay aging in skeletal muscle

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: April 10, 2025

This study investigates how miR-146a-5p, found in adipose tissue-derived small extracellular vesicles (sEV), influences mitochondrial autophagy and its impact on delaying skeletal muscle aging through the targeting of Fbx32. The findings highlight miR-146a-5p as crucial development aging, influencing autophagy, apoptosis, differentiation, proliferation, collectively impacting atrophy. In C2C12 cells, mimics decreased reactive oxygen species (ROS) levels, while enhancing ATP production; conversely, inhibitors had opposite effects. Furthermore, miR-146a-5p-enriched sEV from tissue alleviated atrophy aged mice promoted fiber growth repair by regulating apoptosis. Mechanistically, modulated myoblasts Fbx32 FoxO3 signaling pathway. led to a notable decrease apoptosis-related gene expression, reduced ROS production, elevated levels. conclusion, derived WAT-sEV modulates myoblast ROS, differentiation Fbx32/FoxO3 axis. work presents novel molecular target theoretical framework for developing therapies muscle-related disorders.

Language: Английский

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Endogenous protein S100A14 stabilizes glutaminase to render hepatocellular carcinoma resistant to sorafenib

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: April 11, 2025

Many cases of advanced hepatocellular carcinoma (HCC) are resistant to the widely used drug sorafenib, which worsens prognosis. While many studies have explored how acquired resistance emerges during exposure, mechanism underlying primary before treatment still remain elusive. Single-cell lineage tracing and RNA sequencing were performed identify sorafenib-resistant lineages in HCC. Differential gene expression analysis was employed biomarkers drug-resistant cells. Cell viability colony formation assays adopted assess involvement S100A14 on sorafenib resistance. Co-immunoprecipitation (CO-IP) mass spectrometry conducted uncover downstream targets regulatory mechanisms sorafenib. In vivo mouse xenograft experiments carried out effect or its interacting protein glutaminase (GLS) identified a cluster cells, S100A14, Ca2+-binding protein, determined be critical biomarker for Knockdown significantly increases sensitivity HCC Mechanistically, binds GLS blocks phosphorylation at residues Y308 S314, turn inhibits ubiquitination subsequent degradation. By stabilizing GLS, reduces oxidative stress thereby antagonizing sorafenib-induced apoptosis. Inhibiting improved efficacy against tumors vivo. Our results demonstrate that plays pivotal role promoting by reduce stress, acts as potential therapeutic target enhance patients.

Language: Английский

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Epigenetic modifications in breast cancer: from immune escape mechanisms to therapeutic target discovery

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 17, 2025

Breast cancer (BC) is one of the most prevalent malignant tumors among women globally, with number cases accounting for even more than 1/3 all tumor patients in women. Recent studies have found that incidence BC increasing every year. Despite great progress made treatment, characteristics cells, such as strong immune evasion, easy recurrence and drug resistance, are still main reasons limiting survival patients. Epigenetics becoming an important method to reveal development cancer, mainly through study DNA methylation, histone modification, chromatin structure changes non-coding RNA. In addition, researchers epigenetic markers potential early detection personalized treatment BC. Inhibitors targeting epigenetically modified enzymes effective treating a wide range provide significant patient quality life. Therefore, this review will comprehensively summarize role modifications development. Second, paper focus on summarizing how induce formation microenvironment (TIME) Targeting mechanism action provides new perspectives unravel complex process development, while paving way novel diagnostic therapeutic targets. future, by integrating multi-omics data enable deeper understanding pathogenesis BC, we be able promote overall precision medicine.

Language: Английский

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