Constitutive heterochromatin controls nuclear mechanics, morphology, and integrity through H3K9me3 mediated chromocenter compaction

Nucleus, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 9, 2025

Language: Английский

---

Peripheral heterochromatin tethering is required for chromatin-based nuclear mechanical response

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 16, 2025

The cell nucleus is a mechanically responsive structure that governs how external forces affect chromosomes. Chromatin, particularly transcriptionally inactive heterochromatin, resists nuclear deformations through its mechanical response. However, chromatin also exhibits liquid-like properties, casting ambiguity on the physical mechanisms of chromatin-based elasticity. To determine heterochromatin strengthens response, we performed polymer physics simulations model validated by micromechanical measurements and chromosome conformation capture data. attachment peripheral to lamina required transmit directly elicit elastic Thus, increases in levels increase rigidity increasing linkages between lamina. Crosslinks within such as HP1 α proteins, can stiffen nuclei, but only if peripherally tethered. In contrast, affinity interactions may drive liquid-liquid phase separation do not contribute rigidity. When stretched, gel-like bear stresses deform, while more fluid-like interior euchromatin less perturbed. heterochromatin's internal stiffness regulate mechanics via lamina, enabling mechanosensing measurement nucleus' architecture.

Language: Английский

---

Beyond mechanosensing: How cells sense and shape their physical environment during development

Current Opinion in Cell Biology, Journal Year: 2025, Volume and Issue: 94, P. 102514 - 102514

Published: April 10, 2025

Language: Английский

---

Metabolically intact nuclei are fluidized by the activity of the chromatin remodeling motor BRG1

Biophysical Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Language: Английский

---