Gossiping about death: Apoptosis-induced ERK waves as coordinators of multicellular fate decisions

Seminars in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 171, P. 103615 - 103615

Published: April 24, 2025

Apoptosis is now recognized as a highly dynamic process that involves the release of large set signaling molecules convey information to cells neighboring an apoptotic site. Recent studies in epithelial systems have discovered trigger waves pulses mitogen-activated protein kinase (MAPK) / extracellular signal-regulated (ERK) pathway activity their neighbors. At single-cell level, ERK emerge from MAPK pathway's excitable network properties, such ultrasensitivity and adaptation. cell population apoptosis-induced (AiEWs) propagation across via mechanism mechanical inputs paracrine signaling. AiEWs enable populations dynamically coordinate fate decision during tissue homeostasis development. This spatio-temporal can be hijacked by cancer induce drug-tolerant persister states when apoptosis triggered cytotoxic or targeted therapies, undermining treatment efficacy. In this review, we summarize our current understanding AiEWs, including initiation, propagation, coordination within population. We discuss how relatively simple properties single cells, interactions collective these patterns. highlight implication resistance therapy explore potential strategies target re-sensitize cells. Finally, emerging technologies future directions expand study biological phenomenon.

Language: Английский

Protocol for characterizing cell motility and extracellular signal-regulated kinase dynamics in epithelial monolayers using FRET imaging data

STAR Protocols, Journal Year: 2025, Volume and Issue: 6(2), P. 103816 - 103816

Published: May 8, 2025

Language: Английский