Microgliosis in the Spinal Dorsal Horn Early After Peripheral Nerve Injury Is Associated with Damage to Primary Afferent Aβ-Fibers DOI Creative Commons

Yuto Shibata,

Yuki Matsumoto, Keita Kohno

et al.

Cells, Journal Year: 2025, Volume and Issue: 14(9), P. 666 - 666

Published: May 2, 2025

Neuropathic pain results from a lesion or disease affecting the somatosensory nervous system. Injury to primary afferent nerves leads microgliosis in spinal dorsal horn (SDH), which plays crucial role developing neuropathic pain. Within SDH, fibers broadly project, and microglia are nearly ubiquitously distributed under normal conditions. However, not all react injuries fibers, resulting spatially heterogeneous within SDH. The mechanisms underlying this phenomenon remain elusive. In study, spatial relationship between projections of injured was investigated by generating mice that had expressed tdTomato fourth lumbar root ganglion (L4-DRG) neurons via intra-L4-spinal nerve (SpN) injection adeno-associated viral vectors. After transection L4-SpN, we found SDH selectively occurred innervation territories fibers. denervating transient receptor potential vanilloid 1 (TRPV1)-expressing through intrathecal capsaicin did trigger microgliosis, nor it influence induced L4-SpN injury. Conversely, pharmacological damage myelinated DRG neurons, including Aβ-fibers, sufficient induce microgliosis. Furthermore, injury also gracile nucleus, primarily receives Aβ-fibers. These findings suggest shortly after peripheral is predominantly associated with

Language: Английский

Microgliosis in the Spinal Dorsal Horn Early After Peripheral Nerve Injury Is Associated with Damage to Primary Afferent Aβ-Fibers DOI Creative Commons

Yuto Shibata,

Yuki Matsumoto, Keita Kohno

et al.

Cells, Journal Year: 2025, Volume and Issue: 14(9), P. 666 - 666

Published: May 2, 2025

Neuropathic pain results from a lesion or disease affecting the somatosensory nervous system. Injury to primary afferent nerves leads microgliosis in spinal dorsal horn (SDH), which plays crucial role developing neuropathic pain. Within SDH, fibers broadly project, and microglia are nearly ubiquitously distributed under normal conditions. However, not all react injuries fibers, resulting spatially heterogeneous within SDH. The mechanisms underlying this phenomenon remain elusive. In study, spatial relationship between projections of injured was investigated by generating mice that had expressed tdTomato fourth lumbar root ganglion (L4-DRG) neurons via intra-L4-spinal nerve (SpN) injection adeno-associated viral vectors. After transection L4-SpN, we found SDH selectively occurred innervation territories fibers. denervating transient receptor potential vanilloid 1 (TRPV1)-expressing through intrathecal capsaicin did trigger microgliosis, nor it influence induced L4-SpN injury. Conversely, pharmacological damage myelinated DRG neurons, including Aβ-fibers, sufficient induce microgliosis. Furthermore, injury also gracile nucleus, primarily receives Aβ-fibers. These findings suggest shortly after peripheral is predominantly associated with

Language: Английский

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