Label-Free Liquid Crystal Biosensor Based on Split Aptamers for Detecting Serum Myoglobin DOI

Shaowei Ju,

Shu Zhang, Tingting Yuan

et al.

Langmuir, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 17, 2025

Myoglobin (Mb) is a critical cytoplasmic heme protein and one of the earliest detectable biomarkers following acute myocardial infarction (MI). The rapid precise detection serum Mb holds significant clinical value. This study capitalizes on advantages split aptamer (Apt) sequence, which short effectively minimizes false-positive signals caused by secondary structure probe. It constructs "sandwich"-structured liquid crystal biosensor with an "Apt-target molecule-Apt" configuration for label-free Mb. Apt fragments were immobilized surface glass substrate as capture probes (CP) using triethoxybutanal silane (TEA) linker molecules. When present, other fragment, signal probe (SP), specifically binds reassociates CP, restoring original stem-loop Apt, thereby inducing change in alignment 5CB leads to variations brightness color optical polarizing microscope image, facilitating quantitative method capable detecting concentrations ranging from 0.05 130 ng/mL, minimum limit 0.045 ng/mL. sandwich biosensing offers several advantages, including detection, operational simplicity, high specificity. demonstrates potential biomarkers.

Language: Английский

Trends in Aptasensing and the Enhancement of Diagnostic Efficiency and Accuracy DOI
Mohammad A. Ansari, Damini Verma,

Mohd-Akmal Hamizan

et al.

ACS Synthetic Biology, Journal Year: 2025, Volume and Issue: 14(1), P. 21 - 40

Published: Jan. 6, 2025

The field of healthcare diagnostics is navigating complex challenges driven by evolving patient demographics and the rapid advancement new technologies worldwide. In response to these challenges, biosensors offer distinctive advantages over traditional diagnostic methods, such as cost-effectiveness, enhanced specificity, adaptability, making their integration with point-of-care (POC) platforms more feasible. recent years, aptasensors have significantly evolved in capabilities through emerging microfluidics, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) systems, wearable devices, machine learning (ML), driving progress precision medicine global solutions. Moreover, advancements not only improve accuracy but also hold potential revolutionize early detection, reduce costs, outcomes, especially resource-limited settings. This Account examines key advancements, focusing on how scientific breakthroughs, including artificial intelligence (AI), improved sensitivity precision. Additionally, has enabled real-time monitoring data analysis, fostering advances personalized healthcare. Furthermore, commercialization aptasensor could increase availability clinical settings support use widespread solutions for health challenges. Hence, this review discusses technological improvements, practical uses, prospects while surrounding standardization, validation, interdisciplinary collaboration application. Finally, ongoing efforts address are ensure that can be effectively implemented diverse systems.

Language: Английский

Citations

0
Functionalized borophene with methylene blue tagging for sensitive electrochemical detection of PFOA DOI

Sana Jawaid,

Sadam Hussain Tumrani,

Razium Ali Soomro

et al.

Materials Science and Engineering B, Journal Year: 2025, Volume and Issue: 314, P. 118073 - 118073

Published: Feb. 3, 2025

Language: Английский

Citations

0
Label-Free Liquid Crystal Biosensor Based on Split Aptamers for Detecting Serum Myoglobin DOI

Shaowei Ju,

Shu Zhang, Tingting Yuan

et al.

Langmuir, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 17, 2025

Myoglobin (Mb) is a critical cytoplasmic heme protein and one of the earliest detectable biomarkers following acute myocardial infarction (MI). The rapid precise detection serum Mb holds significant clinical value. This study capitalizes on advantages split aptamer (Apt) sequence, which short effectively minimizes false-positive signals caused by secondary structure probe. It constructs "sandwich"-structured liquid crystal biosensor with an "Apt-target molecule-Apt" configuration for label-free Mb. Apt fragments were immobilized surface glass substrate as capture probes (CP) using triethoxybutanal silane (TEA) linker molecules. When present, other fragment, signal probe (SP), specifically binds reassociates CP, restoring original stem-loop Apt, thereby inducing change in alignment 5CB leads to variations brightness color optical polarizing microscope image, facilitating quantitative method capable detecting concentrations ranging from 0.05 130 ng/mL, minimum limit 0.045 ng/mL. sandwich biosensing offers several advantages, including detection, operational simplicity, high specificity. demonstrates potential biomarkers.

Language: Английский

Citations

0